Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,649	T→A	intergenic (‑8992/‑63569)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,649	0	T	A	100.0%	182.0 / NA	68	intergenic (‑8992/‑63569)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/38);  total (30/38)

TTGCCACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCACTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACCTCAAATAAAGATTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑TTCCATCAAAGTTATAGTTACCTGCAATAGTGCTTACTGTTTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTAGCCGCAACAGCGAGTTTTATCCCAAACTCCTTATAGATAATTGTTGGTGAACCATCTCTATCCCGCTGTGCAAATGGGATTTCTCCCCCCACTAAGATATCGGCAGTTTCACCGGATAACATAGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAAT  >  1/858417‑858888                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                ttGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTgat                                                                                                                                                                                                                               <  1:352253/251‑3 (MQ=255)       cGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCt                                                                                                                                                                                                                         <  1:873763/251‑1 (MQ=255)         aTAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTAc                                                                                                                                                                                                                                   <  1:303848/239‑1 (MQ=255)           aactaactCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAgg                                                                                                                                                                                                                     >  1:709211/1‑251 (MQ=255)            actaactCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGc                                                                                                                                                                                                                    <  1:795596/251‑1 (MQ=255)                  tctTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACg                                                                                                                                                                                                              <  1:343210/251‑1 (MQ=255)                                  cAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTAtttttt                                                                                                                                                                                              >  1:585112/1‑251 (MQ=255)                                    gttgttGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGc                                                                                                                                                                                                                    >  1:465256/1‑227 (MQ=255)                                    gttgttGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTg                                                                                                                                                                                            >  1:42284/1‑251 (MQ=255)                                    gttgttGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTg                                                                                                                                                                                            >  1:456074/1‑251 (MQ=255)                                                  gaagaaAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCaa                                                                                                                                                                              >  1:475889/1‑251 (MQ=255)                                                    agaaagaaCCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACg                                                                                                                                                                                                              <  1:831353/217‑1 (MQ=255)                                                                  aTTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTAttt                                                                                                                                                                                                 <  1:510171/216‑1 (MQ=255)                                                                       gATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAAc                                                                                                                                                          <  1:136677/250‑1 (MQ=255)                                                                          gTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTc                                                                                                                                                        >  1:647903/1‑249 (MQ=255)                                                                             tcCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtat                                                                                                                                                   >  1:475289/1‑251 (MQ=255)                                                                             tcCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtat                                                                                                                                                   >  1:863514/1‑251 (MQ=255)                                                                              ccTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGc                                                                                                                                                                                                                    <  1:415756/185‑1 (MQ=255)                                                                              ccTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGACCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtata                                                                                                                                                  >  1:137056/1‑251 (MQ=255)                                                                               cTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatat                                                                                                                                                 >  1:168175/1‑250 (MQ=255)                                                                                tAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatata                                                                                                                                                <  1:640464/251‑3 (MQ=255)                                                                                 aaaaGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGACTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatatat                                                                                                                                               <  1:612332/251‑4 (MQ=255)                                                                                        gAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAAttg                                                                                                                                          <  1:24560/249‑1 (MQ=255)                                                                                        gAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAAt                                                                                                                                            <  1:836875/247‑1 (MQ=255)                                                                                        gAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAACAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAAttg                                                                                                                                          <  1:426553/249‑1 (MQ=255)                                                                                              tGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatatat                                                                                                                                               >  1:100170/1‑235 (MQ=255)                                                                                               gTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATc                                                                                                                                                                                                                          <  1:374747/162‑1 (MQ=255)                                                                                               gTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAAttg                                                                                                                                          <  1:674654/242‑1 (MQ=255)                                                                                               gTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAAttg                                                                                                                                          >  1:306676/1‑242 (MQ=255)                                                                                               gTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGaa                                                                                                                                  >  1:619642/1‑250 (MQ=255)                                                                                                          cAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATct                                                                                                                      <  1:624027/251‑2 (MQ=255)                                                                                                          cAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATct                                                                                                                      <  1:260867/251‑2 (MQ=255)                                                                                                                cATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTgatt                                                                                                                                                                                                                              <  1:843870/141‑4 (MQ=255)                                                                                                                        cTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATg                                                                                                         <  1:686939/250‑1 (MQ=255)                                                                                                                          gAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGGCTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatata                                                                                                                                                <  1:510606/209‑3 (MQ=255)                                                                                                                          gAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCa                                                                                                                               <  1:373521/226‑1 (MQ=255)                                                                                                                          gAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGa                                                                                                      >  1:298449/1‑251 (MQ=255)                                                                                                                          gAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTA‑ATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGAt                                                                                                     >  1:610478/1‑251 (MQ=255)                                                                                                                                        taataaAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTATGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAAt                                                                                                                                            >  1:161047/1‑199 (MQ=255)                                                                                                                                          ataaAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAAc                                                                                                                                                                             >  1:572207/1‑164 (MQ=255)                                                                                                                                          ataaAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTaa                                                                                      >  1:572700/1‑251 (MQ=255)                                                                                                                                             aaaCTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGat                                                                                   <  1:672845/251‑1 (MQ=255)                                                                                                                                             aaaCTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGat                                                                                   <  1:537119/251‑1 (MQ=255)                                                                                                                                                      ccGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTggcg                                                                                                                                    >  1:476283/1‑191 (MQ=255)                                                                                                                                                              tacttCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCAcctg                                                                  <  1:299047/247‑3 (MQ=255)                                                                                                                                                               acttCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCAcctga                                                                 >  1:458771/4‑248 (MQ=255)                                                                                                                                                                     aaTAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATctc                                                                                                                     <  1:302300/193‑3 (MQ=255)                                                                                                                                                                       tAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATcc                                                                                                                                                               >  1:443323/1‑149 (MQ=255)                                                                                                                                                                          agactttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTA‑TTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGAtt                                                                                                    >  1:213319/5‑204 (MQ=255)                                                                                                                                                                            actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGACTTCC‑TCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                      <  1:699856/247‑1 (MQ=255)                                                                                                                                                                            actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                      <  1:42944/247‑1 (MQ=255)                                                                                                                                                                            actttGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATGTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                      <  1:619916/247‑1 (MQ=255)                                                                                                                                                                                 gCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATgttcgg                                               >  1:503844/1‑251 (MQ=255)                                                                                                                                                                                  cTTTACGGGTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatata                                                                                                                                                <  1:42694/153‑3 (MQ=255)                                                                                                                                                                                         ggTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatat                                                                                                                                                 <  1:605674/145‑2 (MQ=255)                                                                                                                                                                                         ggTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGAt                                                                >  1:385210/1‑226 (MQ=255)                                                                                                                                                                                          gTATTAAAGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAg                                      >  1:132618/1‑251 (MQ=255)                                                                                                                                                                                               aaaGAACGGAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAAc                                   >  1:858954/1‑249 (MQ=255)                                                                                                                                                                                                       gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtata                                                                                                                                                  <  1:487734/130‑1 (MQ=255)                                                                                                                                                                                                       gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTAc                              <  1:610723/246‑1 (MQ=255)                                                                                                                                                                                                       gAATATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCAtt                          <  1:54873/250‑1 (MQ=255)                                                                                                                                                                                                        aaTATTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTAc                        <  1:183680/251‑1 (MQ=255)                                                                                                                                                                                                           atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTtatatat                                                                                                                                               >  1:599827/1‑126 (MQ=255)                                                                                                                                                                                                           atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACttt                     >  1:33771/1‑251 (MQ=255)                                                                                                                                                                                                           atTCCCGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACttt                     >  1:203713/1‑251 (MQ=255)                                                                                                                                                                                                              cccGA‑CTCCCTCAAAGTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGt                  <  1:522610/251‑1 (MQ=255)                                                                                                                                                                                                                              gTTATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGaaa                                                      <  1:591550/200‑1 (MQ=255)                                                                                                                                                                                                                               ttATAGTTACCAGCTATGGTGCTTACTGATTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTGGCAGCAACAGCGAGCTTTATCCCAAACTCCTTATATATAATTGTTGGCGAACCATCTTTATCTCGTTGTGCAAATGGGATTTCACCTCCCACTAAGATATCGGCAGTTTCACCTGATAACATAGAAATGTTCGGTTCGGCCAGAACCTTACCATTACTTTGGTTATCTAAGGCATtaat  <  1:2316/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                TTGCCACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCACTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACCTCAAATAAAGATTTTGCTTTACGGGTATTAAAGAACGGAATATTCCCGA‑TTCCATCAAAGTTATAGTTACCTGCAATAGTGCTTACTGTTTGATCTAAGGCTAAACGAATACGATTATTTTTTTGCAATTTAGCCGCAACAGCGAGTTTTATCCCAAACTCCTTATAGATAATTGTTGGTGAACCATCTCTATCCCGCTGTGCAAATGGGATTTCTCCCCCCACTAAGATATCGGCAGTTTCACCGGATAACATAGAAATGTTCGGTTCGGCCAGAACTTTACCATTACTTTGGTTATCTAAAGCATTAAT  >  1/858417‑858888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑