Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,378	T→C	intergenic (‑8721/‑63840)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,378	0	T	C	100.0%	212.8 / NA	74	intergenic (‑8721/‑63840)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/24);  total (50/24)

GTTGTGCAACAGTCGTATCGTTAACAAATTCACTGATTGCAAATGTTGTTACCGGGGCGGTAATTGAGGCCAATGCAATGGAGGCCGTGATAACTAATTTTCTCATTGTATGAACCCACTATTTTTTAAGAAGTTAGATGTTAATGTTTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAATAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCCACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCACTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACCTCAAATAAAGATTTTGCTTTACGGGTATTAAAGAACGGAATATTC  >  1/858154‑858622                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     gTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTta                                                                                                                                                                                                                            >  1:569516/1‑251 (MQ=255)    gtgCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTtacta                                                                                                                                                                                                                         >  1:315531/1‑251 (MQ=255)             tCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCACTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTg                                                                                                                                                                                                                <  1:26189/251‑1 (MQ=255)                                      tGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCtttttt                                                                                                                                                                                       <  1:743436/251‑1 (MQ=255)                                        cAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTAt                                                                                                                                                                                     >  1:277222/1‑251 (MQ=255)                                                          cgatAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGt                                                                                                                                                                                                               <  1:47207/204‑1 (MQ=255)                                                          cgatAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCat                                                                                                                                                                   <  1:25320/248‑2 (MQ=255)                                                                     gCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGgaagaa                                                                                                                                                         <  1:524493/250‑1 (MQ=255)                                                                         atgcaatgGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAagaaagaa                                                                                                                                                     >  1:390986/1‑250 (MQ=255)                                                                         atgcaatgGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAagaaagaa                                                                                                                                                     >  1:677226/1‑250 (MQ=255)                                                                         atgcaatgGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAagaaagaa                                                                                                                                                     >  1:299605/1‑250 (MQ=255)                                                                                     ccGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTAt                                                                                                                                                                                     >  1:47135/1‑206 (MQ=255)                                                                                     ccGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAgttgt                                                                                                                                                                        >  1:477827/1‑219 (MQ=255)                                                                                              cTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCttttt                                                                                                                                                                                                                                           >  1:160012/1‑143 (MQ=255)                                                                                                    ttCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAagag                                                                                                                         <  1:584154/251‑1 (MQ=255)                                                                                                            gTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATg                                                                                                                                      >  1:350451/1‑230 (MQ=255)                                                                                                            gTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTg                                                                                                                 >  1:633376/1‑251 (MQ=255)                                                                                                            gTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTg                                                                                                                 >  1:397528/1‑251 (MQ=255)                                                                                                                 aaCCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTT‑CATTACGGaagaaaga                                                                                                                                                      <  1:893751/208‑1 (MQ=255)                                                                                                                              ttAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGAtaactaac                                                                                                                                                                                               <  1:521202/155‑1 (MQ=255)                                                                                                                                   aagTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTg                                                                                           <  1:283624/250‑1 (MQ=255)                                                                                                                                     gTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAag                                                                                                                           <  1:374242/216‑1 (MQ=255)                                                                                                                                     gTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAag                                                                                                                           <  1:191150/216‑1 (MQ=255)                                                                                                                                     gTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAg                                                                                         >  1:156225/1‑250 (MQ=255)                                                                                                                                          aTGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGt                                                                                                                                                                                                               >  1:328686/1‑127 (MQ=255)                                                                                                                                          aTGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAag                                                                                                                           <  1:19574/211‑1 (MQ=255)                                                                                                                                          aTGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTg                                                                                      <  1:627371/248‑1 (MQ=255)                                                                                                                                          aTGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAAt                                                                                   >  1:734551/1‑251 (MQ=255)                                                                                                                                          aTGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAAt                                                                                   >  1:570967/1‑251 (MQ=255)                                                                                                                                          aTGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAAt                                                                                   <  1:343454/251‑1 (MQ=255)                                                                                                                                           tGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCat                                                                                                                                                                   <  1:673363/170‑2 (MQ=255)                                                                                                                                           tGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATa                                                                                  >  1:713673/1‑251 (MQ=255)                                                                                                                                            gTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATaa                                                                                 >  1:19889/1‑251 (MQ=255)                                                                                                                                              tAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGc                                                                               <  1:418738/251‑1 (MQ=255)                                                                                                                                               aaTGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGcc                                                                              <  1:348098/251‑1 (MQ=255)                                                                                                                                                  gtcttGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGAtaact                                                                                                                                                                                                  <  1:759512/129‑1 (MQ=255)                                                                                                                                                  gtcttGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAgg                                                                                                  <  1:402423/225‑1 (MQ=255)                                                                                                                                                  gtcttGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCAc                                                                            >  1:547811/4‑250 (MQ=255)                                                                                                                                                       gTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGcta                                                                                                                                                                                                          >  1:169512/1‑117 (MQ=255)                                                                                                                                                       gTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTGTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGataa                                                                      >  1:612029/1‑251 (MQ=255)                                                                                                                                                       gTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCAGTTTTTTCAAAATCAGGGTAAACAGCATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGataa                                                                      >  1:148992/1‑251 (MQ=255)                                                                                                                                                         aataaACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGATTTTTCAAAATCAGGGTAAACAATATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCTACCGataata                                                                    >  1:890265/1‑251 (MQ=255)                                                                                                                                                              aCATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATaaaa                                                                 >  1:283383/1‑249 (MQ=255)                                                                                                                                                                 ttCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAag                                                                                                                           >  1:254106/1‑188 (MQ=255)                                                                                                                                                                 ttCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGcc                                                                              >  1:281560/1‑233 (MQ=255)                                                                                                                                                                 ttCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACttt                                                             >  1:629169/1‑250 (MQ=255)                                                                                                                                                                 ttCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACttt                                                             >  1:443364/1‑250 (MQ=255)                                                                                                                                                                   cTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTAt                                                                                                                                                                                     >  1:475864/1‑128 (MQ=255)                                                                                                                                                                   cTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTtct                                                           >  1:575743/1‑250 (MQ=255)                                                                                                                                                                   cTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTtct                                                           >  1:621188/1‑250 (MQ=255)                                                                                                                                                                   cTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTtct                                                           >  1:56193/1‑250 (MQ=255)                                                                                                                                                                      tAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAgttg                                                                                                                                                                         <  1:460959/137‑1 (MQ=255)                                                                                                                                                                           gAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATg                                                                                                                                      <  1:432591/167‑1 (MQ=255)                                                                                                                                                                           gAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAg                                                    >  1:498393/1‑249 (MQ=255)                                                                                                                                                                           gAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAg                                                    >  1:301665/1‑249 (MQ=255)                                                                                                                                                                             gTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTa                                                 >  1:61851/1‑250 (MQ=255)                                                                                                                                                                             gTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTa                                                 >  1:872205/1‑250 (MQ=255)                                                                                                                                                                                gTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTActt                                              >  1:56696/1‑248 (MQ=255)                                                                                                                                                                                gTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTActt                                              >  1:726876/1‑248 (MQ=255)                                                                                                                                                                                gTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTActt                                              >  1:822528/1‑248 (MQ=255)                                                                                                                                                                                  aTGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCaa                                           >  1:367296/1‑251 (MQ=255)                                                                                                                                                                                    ggAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAAt                                         >  1:118996/1‑251 (MQ=255)                                                                                                                                                                                     gaagaaTCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCaa                                                                                                    <  1:692825/191‑1 (MQ=255)                                                                                                                                                                                     gaagaaTCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATa                                        >  1:523771/1‑251 (MQ=255)                                                                                                                                                                                        gaaTCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAg                                     >  1:266667/1‑251 (MQ=255)                                                                                                                                                                                          aTCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGac                                   <  1:698302/251‑2 (MQ=255)                                                                                                                                                                                             tttCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGActtt                                >  1:578680/1‑251 (MQ=255)                                                                                                                                                                                                ccATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTc                                                                                                >  1:92958/1‑184 (MQ=255)                                                                                                                                                                                                          ggTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTAt                   >  1:73225/1‑251 (MQ=255)                                                                                                                                                                                                               tttCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAg              >  1:40447/1‑251 (MQ=255)                                                                                                                                                                                                                       tCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGt                                                                                                 >  1:450934/1‑160 (MQ=255)                                                                                                                                                                                                                          gggTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATAtt   >  1:730686/1‑251 (MQ=255)                                                                                                                                                                                                                            gTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAg              >  1:87451/1‑238 (MQ=255)                                                                                                                                                                                                                            gTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCTACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCATTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACTTCAAATAAAGACTTTGCTTTACGGGTATTAAAGAACGGAATATTc  <  1:402589/250‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GTTGTGCAACAGTCGTATCGTTAACAAATTCACTGATTGCAAATGTTGTTACCGGGGCGGTAATTGAGGCCAATGCAATGGAGGCCGTGATAACTAATTTTCTCATTGTATGAACCCACTATTTTTTAAGAAGTTAGATGTTAATGTTTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAATAACATCTTTTTCATTTACCGGTTTTACTACATTTACTGTTGCCACGATAACTAACTCTTTTTTATCGCGCTCAGTTGTTGCACTACGGAAGAAAGAACCTAAAATTGGGATGTCTCCTAAAAGAGGAACTTTGTTTATACCTTCAAGGTCATTTGAGCTGAATAAGCCACCGATAATAAAACTTTCTCCGTTAGCTACCTCAAATAAAGATTTTGCTTTACGGGTATTAAAGAACGGAATATTC  >  1/858154‑858622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑