Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,186	C→T	intergenic (‑8529/‑64032)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,186	0	C	T	100.0%	137.8 / NA	53	intergenic (‑8529/‑64032)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/29);  total (24/29)

CGGCTTTCTTTGCCGTAGCATCACGCCAAATTATATTGATTTGCTTTTGGTTGTTACTACCTATATCACGAATAACGGAATTAAATAAATTACTATAACTGACATTAGAGTAATCCGGTAATACATAACGATCGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTTAACAAATTCACTGATTGCAAATGTTGTTACCGGGGCGGTAATTGAGGCCAATGCAATGGAGGCCGTGATAACTAATTTTCTCATTGTATGAACCCACTATTTTTTAAGAAGTTAGATGTTAATGTTTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAATAACATCTTTTTCATTTACCGGTTTTACTACATTTACTG  >  1/857983‑858416                                                                                                                                                                                                            |                                                                                                                                                                                                                                       cGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCcaatgcaat                                                                                                                                                                                          <  1:228237/250‑1 (MQ=255)   gCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATgg                                                                                                                                                                                        <  1:578163/250‑1 (MQ=255)      ttctttGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAgg                                                                                                                                                                                     >  1:767128/1‑250 (MQ=255)      ttctttGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGc                                                                                                                                                                                    <  1:497763/251‑1 (MQ=255)                        cGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATgg                                                                                                                                                                                        <  1:24003/229‑1 (MQ=255)                            aaaTGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGt                                                                                                                                                                                 <  1:676897/232‑1 (MQ=255)                                         ttgtttttGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACt                                                                                                                                                 <  1:211/247‑1 (MQ=255)                                             ttttGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTAttc                                                                                                                                             <  1:504045/251‑2 (MQ=255)                                                      ttACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCcaatgc                                                                                                                                                                                             >  1:222257/1‑194 (MQ=255)                                                      ttACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTaagaag                                                                                                                                    >  1:508324/1‑251 (MQ=255)                                                                                attgaataaatTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAAc                                                                                                          <  1:801448/247‑1 (MQ=255)                                                                                             cTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTtc                                                                                                                                                                   <  1:121544/181‑1 (MQ=255)                                                                                                      aCATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAAc                                                                                                          >  1:882225/1‑229 (MQ=255)                                                                                                       cATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAAc                                                                                                          <  1:160/228‑1 (MQ=255)                                                                                                       cATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGga                                                                                   >  1:438033/1‑251 (MQ=255)                                                                                                           agaatAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGtaa                                                                                                               >  1:855606/5‑219 (MQ=255)                                                                                                            gaatAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAAt                                                                              >  1:143061/4‑251 (MQ=255)                                                                                                                 aTCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTtctc                                                                                                                                                                 <  1:528979/163‑1 (MQ=255)                                                                                                                    cGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTc                                                                                                      <  1:359177/219‑1 (MQ=255)                                                                                                                    cGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATgg                                                                                    >  1:761327/1‑237 (MQ=255)                                                                                                                     ggTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGt                                                                                                                            >  1:465830/1‑196 (MQ=255)                                                                                                                      gTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATg                                                                                                                       <  1:382933/200‑1 (MQ=255)                                                                                                                      gTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTg                                                                    >  1:610757/1‑251 (MQ=255)                                                                                                                        aaTACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATg                                                                                                                             >  1:600156/1‑192 (MQ=255)                                                                                                                        aaTACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACgg                                                                                              >  1:407852/1‑223 (MQ=255)                                                                                                                         aTACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGga                                                                                   >  1:386682/1‑233 (MQ=255)                                                                                                                         aTACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGtt                                                                  >  1:685583/1‑250 (MQ=255)                                                                                                                           aCATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAAc                                                                                                <  1:5876/218‑1 (MQ=255)                                                                                                                           aCATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGgaag                                                                                 <  1:758341/233‑1 (MQ=255)                                                                                                                            cATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTc                                                                 <  1:830495/248‑1 (MQ=255)                                                                                                                              tAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAAc                                                                                                          <  1:730276/205‑1 (MQ=255)                                                                                                                              tAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGACCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGtt                                                            <  1:676821/251‑1 (MQ=255)                                                                                                                                aCGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGtttt                                                          >  1:480649/1‑251 (MQ=255)                                                                                                                                 cGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGtt                                                            <  1:424317/248‑1 (MQ=255)                                                                                                                                   attgACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTaaga                                                                                                                                      <  1:868363/169‑1 (MQ=255)                                                                                                                                   attgACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGgaag                                                                                 <  1:500870/222‑1 (MQ=255)                                                                                                                                    ttgACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGcc                                                                                                                                                                                   >  1:372559/3‑126 (MQ=255)                                                                                                                                    ttgACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATg                                                                                                                       >  1:465160/3‑186 (MQ=255)                                                                                                                                    ttgACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAAc                                                                                                >  1:845507/3‑209 (MQ=255)                                                                                                                                      gACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATc                                                                             >  1:229865/1‑226 (MQ=255)                                                                                                                                        cTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGtaa                                                                                                               <  1:542475/190‑1 (MQ=255)                                                                                                                                           aCTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGgaag                                                                                 <  1:750070/217‑1 (MQ=255)                                                                                                                                              gtgtACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAAcc                                                                                                                                                     <  1:309365/146‑1 (MQ=255)                                                                                                                                              gtgtACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGt                                            >  1:886097/1‑251 (MQ=255)                                                                                                                                                             cACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTaag                                                                                                                                       <  1:353717/145‑1 (MQ=255)                                                                                                                                                               cTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATgg                                                                                                                                                                                        <  1:759196/94‑1 (MQ=255)                                                                                                                                                               cTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAAcc                                                                                                                                                     <  1:867137/129‑1 (MQ=255)                                                                                                                                                                 aTTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTaaga                                                                                                                                      >  1:503614/1‑142 (MQ=255)                                                                                                                                                                 aTTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGtaa                                                                                                               <  1:735330/165‑1 (MQ=255)                                                                                                                                                                    ttGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGcc                                                                                                                                                                                                  >  1:681778/1‑79 (MQ=255)                                                                                                                                                                            gTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTta              >  1:569516/1‑251 (MQ=255)                                                                                                                                                                               gtgCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTtacta           >  1:315531/1‑251 (MQ=255)                                                                                                                                                                                        tCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCACTGTATGAACCCACTATTCTTTAAGAAGTTAGATGTTAATGTCTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAACAACATCTTTTTCATTTACCGGTTTTACTACATTTACTg  <  1:26189/251‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                       CGGCTTTCTTTGCCGTAGCATCACGCCAAATTATATTGATTTGCTTTTGGTTGTTACTACCTATATCACGAATAACGGAATTAAATAAATTACTATAACTGACATTAGAGTAATCCGGTAATACATAACGATCGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTTAACAAATTCACTGATTGCAAATGTTGTTACCGGGGCGGTAATTGAGGCCAATGCAATGGAGGCCGTGATAACTAATTTTCTCATTGTATGAACCCACTATTTTTTAAGAAGTTAGATGTTAATGTTTTGTAATAAACATTCTTTAACGGAGTCGTATGGAAGAATCTTTCCATTGTTCCGGTTTTTTCAAAATCAGGGTAAATAACATCTTTTTCATTTACCGGTTTTACTACATTTACTG  >  1/857983‑858416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑