Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,065	A→G	intergenic (‑8408/‑64153)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,065	0	A	G	100.0%	100.1 / NA	36	intergenic (‑8408/‑64153)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

ACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATATAGATTGGATATGTTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATAGATTTAGAGGTAATACCATTTTTAATCAATTTTGTACGAATTTTCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATTATATTGATTTGCTTTTGGTTGTTACTACCTATATCACGAATAACGGAATTAAATAAATTACTATAACTGACATTAGAGTAATCCGGTAATACATAACGATCGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTTAACAAATTCACTGATTGCAAATGTTGTTACCGGGGCGGTAATTGAGGCCAATGCAATGGAGGCCGTGATAACTAATTTTCTCATTGTATGAACCCACTATTTTTTAAGAAG  >  1/857823‑858286                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              actactTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAAtt                                                                                                                                                                                                                       <  1:696406/251‑1 (MQ=255)      ttCTCCGGTACGATATGCACAGGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACtat                                                                                                                                                                                                                  <  1:475489/251‑1 (MQ=255)               aCGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACAt                                                                                                                                                                                                         >  1:1760/1‑251 (MQ=255)                                    tctctgcgcAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTagaat                                                                                                                                                                                                   >  1:295766/6‑234 (MQ=255)                                              aTTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTagaa                                                                                                                                                                                                    >  1:744994/1‑224 (MQ=255)                                                        cTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTg                                                                                                                                                                                                             >  1:266230/1‑206 (MQ=255)                                                        cTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTagaat                                                                                                                                                                                                   <  1:88097/216‑3 (MQ=255)                                                         ttCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTAc                                                                                                                                                               <  1:530630/251‑1 (MQ=255)                                                          tCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTaa                                                                                                                                                                                         >  1:332138/1‑224 (MQ=255)                                                            aaTATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGAt                                                                                                                                                            >  1:331429/1‑251 (MQ=255)                                                            aaTATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGAt                                                                                                                                                            <  1:741021/251‑1 (MQ=255)                                                            aaTATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACCATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGAt                                                                                                                                                            <  1:470825/251‑1 (MQ=255)                                                                      ggATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTagaat                                                                                                                                                                                                   >  1:737580/1‑200 (MQ=255)                                                                        atatatTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACtg                                                                                                                                                                    <  1:832398/231‑1 (MQ=255)                                                                        atatatTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTAt                                                                                                                                                <  1:745632/251‑1 (MQ=255)                                                                               tttGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTAc                                                                                                                                         <  1:107084/251‑1 (MQ=255)                                                                                  gCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCgg                                                                                                                                      >  1:625946/1‑251 (MQ=255)                                                                                           gCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAAc                                                                                                                                                                                 <  1:802737/199‑1 (MQ=255)                                                                                           gCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAAc                                                                                                                                                                                 >  1:163066/1‑199 (MQ=255)                                                                                            cTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAAc                                                                                                                             <  1:240469/250‑1 (MQ=255)                                                                                                         gTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCg                                                                                                                   >  1:90780/1‑247 (MQ=255)                                                                                                                 aTTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACtg                                                                                                                                                                    >  1:868739/1‑190 (MQ=255)                                                                                                                 aTTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCa                                                                                                       >  1:467484/1‑251 (MQ=255)                                                                                                                                                    cGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAAc                                                                                                                                                                      >  1:486569/1‑153 (MQ=255)                                                                                                                                                      aaTTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATaa                                                                                                                                                                                                                <  1:682100/109‑1 (MQ=255)                                                                                                                                                                 cGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCcaatgcaat                                                        <  1:228237/250‑1 (MQ=255)                                                                                                                                                                   gCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATgg                                                      <  1:578163/250‑1 (MQ=255)                                                                                                                                                                      ttctttGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAgg                                                   >  1:767128/1‑250 (MQ=255)                                                                                                                                                                      ttctttGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGc                                                  <  1:497763/251‑1 (MQ=255)                                                                                                                                                                                        cGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATgg                                                      <  1:24003/229‑1 (MQ=255)                                                                                                                                                                                            aaaTGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGt                                               <  1:676897/232‑1 (MQ=255)                                                                                                                                                                                                         ttgtttttGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACt               <  1:211/247‑1 (MQ=255)                                                                                                                                                                                                             ttttGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTAttc           <  1:504045/251‑2 (MQ=255)                                                                                                                                                                                                                      ttACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCcaatgc                                                           >  1:222257/1‑194 (MQ=255)                                                                                                                                                                                                                      ttACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTCAACAAATTCATTGATTGCAAATGTTTTTGCCGGAACGATAATTGAGGCCAATGCAATGGAGGCCGTAATAACTAATTTTCTCATTGTATGAACCCACTATTCTTTaagaag  >  1:508324/1‑251 (MQ=255)                                                                                                                                                                                                                               atatCACGAATGACAGAATTGAATAAATTACTATAACTGACATTAGAATAATCCGGTAATACATAACGATTGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAAc                                                                                                                             <  1:895490/119‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              ACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATATAGATTGGATATGTTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATAGATTTAGAGGTAATACCATTTTTAATCAATTTTGTACGAATTTTCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATTATATTGATTTGCTTTTGGTTGTTACTACCTATATCACGAATAACGGAATTAAATAAATTACTATAACTGACATTAGAGTAATCCGGTAATACATAACGATCGACTAACTGTGTACGATTAAATTCACTATTTTGTACCGGTTGTGCAACAGTCGTATCGTTAACAAATTCACTGATTGCAAATGTTGTTACCGGGGCGGTAATTGAGGCCAATGCAATGGAGGCCGTGATAACTAATTTTCTCATTGTATGAACCCACTATTTTTTAAGAAG  >  1/857823‑858286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑