Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	857,859	T→C	intergenic (‑8202/‑64359)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	857,859	0	T	C	100.0%	152.6 / NA	55	intergenic (‑8202/‑64359)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/24);  total (31/24)

AGTACGTAGAATTTGTGCAACTTCACCTTGGATATCATCACGGCTTGACACTATAGTAATAGTGCGAGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATATAGATTGGATATGTTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATAGATTTAGAGGTAATACCATTTTTAATCAATTTTGTACGAATTTTCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATTATATTGATTTGCTTTTGGTTGTTACTACCTATATCACGAATAACGGAATTAAATAAATTACTATAACTGACAT  >  1/857633‑858087                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     aGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCaa                                                                                                                                                                                                              >  1:649084/1‑251 (MQ=255)  gTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAat                                                                                                                                                                                                             >  1:690801/1‑251 (MQ=255)   tACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAata                                                                                                                                                                                                            >  1:286019/1‑251 (MQ=255)    aCGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAatat                                                                                                                                                                                                           <  1:170678/251‑1 (MQ=255)          agtttGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAt                                                                                                                                                                                                       <  1:659905/247‑1 (MQ=255)          agtttGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAt                                                                                                                                                                                                       <  1:200446/247‑1 (MQ=255)            tttGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGGGCAATTTGAGCTACTTCAATATAGATTGGa                                                                                                                                                                                                   <  1:101749/251‑1 (MQ=255)                    aCTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATAtttt                                                                                                                                                                                           >  1:28804/1‑251 (MQ=255)                                               gatacTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCt                                                                                                                                                                                                                     <  1:246256/195‑1 (MQ=255)                                                     acaGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAtt                                                                                                                                                                                                      >  1:484886/3‑207 (MQ=255)                                                     acaGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAAtgga                                                                                                                                                          >  1:453893/3‑248 (MQ=255)                                                      caGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGAt                                                                                                                                                         >  1:377486/2‑251 (MQ=255)                                                        gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCt                                                                                                                                                                                                                     >  1:233191/1‑189 (MQ=255)                                                        gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGAttt                                                                                                                                                       >  1:862273/1‑251 (MQ=255)                                                        gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGAttt                                                                                                                                                       >  1:244043/1‑251 (MQ=255)                                                              gtacgtgCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAgg                                                                                                                                                  >  1:482277/7‑250 (MQ=255)                                                               tacgtgCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAgg                                                                                                                                                  <  1:810929/244‑1 (MQ=255)                                                                      aCTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCAt                                                                                                                                         <  1:117945/251‑1 (MQ=255)                                                                              ccGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAAt                                                                                                                                  >  1:349204/1‑250 (MQ=255)                                                                                             gATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAAtt                                                                                                                  >  1:299625/1‑251 (MQ=255)                                                                                                  aGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAAtgg                                                                                                                                                           <  1:653752/205‑3 (MQ=255)                                                                                                   gTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCAtt                                                                                                            >  1:668755/1‑251 (MQ=255)                                                                                                       taaCATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAatat                                                                                                                                                                                                           >  1:647247/1‑152 (MQ=255)                                                                                                          cATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGc                                                                                                                                                                                        <  1:612077/168‑1 (MQ=255)                                                                                                             aaaaaaTACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGcttt                                                                                                    >  1:392321/1‑249 (MQ=255)                                                                                                                      cTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCgg                                                                                                        >  1:679031/1‑236 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCt                                                                                                                                                                                                                     >  1:544732/1‑124 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAt                                                                                                                                                                                                       >  1:554554/1‑138 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGata                                                                                                                                                                                                 <  1:524249/144‑1 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTag                                                                                                                                                     >  1:352969/1‑188 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATc                                                                                                                                 >  1:405803/1‑208 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCAt                                                                                      <  1:108104/251‑1 (MQ=255)                                                                                                                         cTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCAt                                                                                      >  1:111727/1‑251 (MQ=255)                                                                                                                           aTTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGctttc                                                                                                   >  1:207813/1‑236 (MQ=255)                                                                                                                            ttAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCAc                                                                                   <  1:594764/251‑1 (MQ=255)                                                                                                                                  cTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCaaa                                                                             >  1:41621/1‑251 (MQ=255)                                                                                                                                     cAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAtt                                                                                                                                                                                                      <  1:507321/127‑1 (MQ=255)                                                                                                                                     cAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAAtg                                                                           >  1:219089/1‑249 (MQ=255)                                                                                                                                           aaCACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTg                                                                     <  1:573292/250‑1 (MQ=255)                                                                                                                                           aaCACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTg                                                                     <  1:30624/250‑1 (MQ=255)                                                                                                                                                   gATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCt                                                                                                                                                                                                                     <  1:644313/98‑1 (MQ=255)                                                                                                                                                   gATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAatat                                                                                                                                                                                                           <  1:793134/108‑1 (MQ=255)                                                                                                                                                      tACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCa                                                                                    >  1:49029/1‑224 (MQ=255)                                                                                                                                                        cTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGt                                                                                                                                                                 >  1:230080/1‑145 (MQ=255)                                                                                                                                                                tGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTtactac                                               <  1:507635/251‑1 (MQ=255)                                                                                                                                                                  cacaAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTAcc                                              <  1:46376/250‑1 (MQ=255)                                                                                                                                                                  cacaAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTAcc                                              <  1:403827/250‑1 (MQ=255)                                                                                                                                                                    caAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCtat                                           >  1:385612/1‑251 (MQ=255)                                                                                                                                                                        aagTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCa                                       <  1:751871/251‑1 (MQ=255)                                                                                                                                                                                               actactTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAAtt                <  1:696406/251‑1 (MQ=255)                                                                                                                                                                                                    ttCTCCGGTACGATATGCACAGGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACtat           <  1:475489/251‑1 (MQ=255)                                                                                                                                                                                                             aCGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAAt                          >  1:259751/1‑227 (MQ=255)                                                                                                                                                                                                             aCGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCACGAATGACAGAATTGAATAAATTACTATAACTGACAt  >  1:1760/1‑251 (MQ=255)                                                                                                                                                                                                               gATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTACCTATATCa                                       <  1:680285/212‑1 (MQ=255)                                                                                                                                                                                                                    gCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGATTTAGAGGCGATACCATTTTTAATCAATTTTGTACGAATTTCCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATGATATTGATTTGTTTTTGGTTGTTACTAcc                                              >  1:27961/1‑200 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     AGTACGTAGAATTTGTGCAACTTCACCTTGGATATCATCACGGCTTGACACTATAGTAATAGTGCGAGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATATAGATTGGATATGTTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATAGATTTAGAGGTAATACCATTTTTAATCAATTTTGTACGAATTTTCATTGCGGCTTTCTTTGCCGTAGCATCACGCCAAATTATATTGATTTGCTTTTGGTTGTTACTACCTATATCACGAATAACGGAATTAAATAAATTACTATAACTGACAT  >  1/857633‑858087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑