Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 857,699 A→T intergenic (‑8042/‑64519) unknown ← / → unknown tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1857,6990AT100.0% 130.3 / NA 49intergenic (‑8042/‑64519)unknown/unknowntRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/26);  total (23/26)

GTGGTACTATACTAAAAACTCGTTCGACAATAGTCTTTATGTTAGTCTCATGTGTGATATCAATAATAACCCCTACAGTGTCTTCTGCTGAAAATGAAATTTCATCAGATGAGGTGAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAATTTGTGCAACTTCACCTTGGATATCATCACGGCTTGACACTATAGTAATAGTGCGAGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATATAGATTGGATATGTTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATAGATTT  >  1/857476‑857938
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
gtgGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGa                                                                                                                                                                                                                      >  1:78445/1‑251 (MQ=255)
        acactaAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGtaa                                                                                                                                                                                                              <  1:282716/249‑1 (MQ=255)
         cactaAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAAAGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGtaat                                                                                                                                                                                                             <  1:228663/250‑1 (MQ=255)
                                    ttATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                                                                                  <  1:625355/251‑1 (MQ=255)
                                    ttATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                                                                                  <  1:408840/251‑1 (MQ=255)
                                    ttATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                                                                                  <  1:324068/251‑1 (MQ=255)
                                                 aTGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAacac                                                                                                                                                                     <  1:486786/251‑1 (MQ=255)
                                                          atCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCg                                                                                                                                                                                                                                    <  1:839672/179‑1 (MQ=255)
                                                              ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGAt                                                                                                                                                                                                                     <  1:689380/190‑1 (MQ=255)
                                                              ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                                                                                  <  1:395769/225‑1 (MQ=255)
                                                              ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACtttt                                                                                                                                                        >  1:272975/1‑251 (MQ=255)
                                                               taataaTCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTa                                                                                                                                                       >  1:569616/1‑251 (MQ=255)
                                                                 ataaTCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAg                                                                                                                                                     <  1:168698/251‑1 (MQ=255)
                                                                     tcccTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATAc                                                                                                                                                                                               >  1:342124/2‑205 (MQ=255)
                                                                     tcccTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAg                                                                                                                                                     >  1:250263/2‑247 (MQ=255)
                                                                        cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCAc                                                                                                                                                                                                                                             <  1:789176/156‑1 (MQ=255)
                                                                        cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGta                                                                                                                                                                                                               >  1:565482/1‑186 (MQ=255)
                                                                        cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAAc                                                                                                                                                                                                          >  1:434242/1‑191 (MQ=255)
                                                                        cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATAcc                                                                                                                                                                                              >  1:243357/1‑203 (MQ=255)
                                                                        cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTg                                                                                                                                                                          <  1:558359/223‑1 (MQ=255)
                                                                         tACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACaag                                                                                                                                             <  1:799743/251‑1 (MQ=255)
                                                                          aCAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACaaga                                                                                                                                            >  1:527924/1‑251 (MQ=255)
                                                                                 cttctGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCa                                                                                                                                     >  1:50804/1‑251 (MQ=255)
                                                                                           aaaTGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTGCAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCt                                                                                                                                                                                                                   <  1:75352/163‑1 (MQ=255)
                                                                                                      catcaGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAg                                                                                                                                                     >  1:798203/1‑214 (MQ=255)
                                                                                                           gacgAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                                                                                  <  1:648798/177‑1 (MQ=255)
                                                                                                                  taaaaaaaTCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGa                                                                                                    <  1:289557/249‑1 (MQ=255)
                                                                                                                   aaaaaaaTCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTg                                                                                                                                                                          >  1:208536/2‑180 (MQ=255)
                                                                                                                         aTCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGcac                                                                                             <  1:81237/251‑1 (MQ=255)
                                                                                                                                     aaTCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGtc                                                                                 <  1:113693/251‑2 (MQ=255)
                                                                                                                                              atTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACttt                                                                                                                                                         >  1:138081/1‑170 (MQ=255)
                                                                                                                                                             aGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCaa                                                         >  1:649084/1‑251 (MQ=255)
                                                                                                                                                              gTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAat                                                        >  1:690801/1‑251 (MQ=255)
                                                                                                                                                               tACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAata                                                       >  1:286019/1‑251 (MQ=255)
                                                                                                                                                                aCGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAatat                                                      <  1:170678/251‑1 (MQ=255)
                                                                                                                                                                      agtttGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAt                                                  <  1:659905/247‑1 (MQ=255)
                                                                                                                                                                      agtttGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAt                                                  <  1:200446/247‑1 (MQ=255)
                                                                                                                                                                        tttGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGGGCAATTTGAGCTACTTCAATATAGATTGGa                                              <  1:101749/251‑1 (MQ=255)
                                                                                                                                                                                aCTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATAtttt                                      >  1:28804/1‑251 (MQ=255)
                                                                                                                                                                                                           gatacTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCt                                                                <  1:246256/195‑1 (MQ=255)
                                                                                                                                                                                                                 acaGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGAtt                                                 >  1:484886/3‑207 (MQ=255)
                                                                                                                                                                                                                 acaGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAAtgga     >  1:453893/3‑248 (MQ=255)
                                                                                                                                                                                                                  caGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGAt    >  1:377486/2‑251 (MQ=255)
                                                                                                                                                                                                                    gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTg                                                                                                                                                                          <  1:556573/83‑1 (MQ=255)
                                                                                                                                                                                                                    gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTa                                                                                                                                                             <  1:515058/96‑1 (MQ=255)
                                                                                                                                                                                                                    gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACttt                                                                                                                                                         <  1:893595/100‑1 (MQ=255)
                                                                                                                                                                                                                    gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCt                                                                >  1:233191/1‑189 (MQ=255)
                                                                                                                                                                                                                    gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGAttt  >  1:244043/1‑251 (MQ=255)
                                                                                                                                                                                                                    gTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAATATAGATTGGATATATTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATGGAttt  >  1:862273/1‑251 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
GTGGTACTATACTAAAAACTCGTTCGACAATAGTCTTTATGTTAGTCTCATGTGTGATATCAATAATAACCCCTACAGTGTCTTCTGCTGAAAATGAAATTTCATCAGATGAGGTGAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAATTTGTGCAACTTCACCTTGGATATCATCACGGCTTGACACTATAGTAATAGTGCGAGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATATAGATTGGATATGTTTTTGCTTTATGTGCTTTTTTCTCTAAGTAAATAGATTT  >  1/857476‑857938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑