Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	857,643	A→G	intergenic (‑7986/‑64575)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	857,643	0	A	G	100.0%	194.4 / NA	68	intergenic (‑7986/‑64575)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/41);  total (27/41)

AAGGATGCCCTCATCTAGTAGTTTTTGTGATAAAGAGATGGAGTCACTATCACCGATAACACAACACCATACATTTTGTGGTACTATACTAAAAACTCGTTCGACAATAGTCTTTATGTTAGTCTCATGTGTGATATCAATAATAACCCCTACAGTGTCTTCTGCTGAAAATGAAATTTCATCAGATGAGGTGAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAATTTGTGCAACTTCACCTTGGATATCATCACGGCTTGACACTATAGTAATAGTGCGAGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATAT  >  1/857399‑857886                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aagGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATAACGCAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTtgtg                                                                                                                                                                                                                                               <  1:619879/251‑1 (MQ=255)            cATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCAc                                                                                                                                                                                                                                      >  1:58988/1‑249 (MQ=255)                     gtTTTTGTGATAAAGAGATTGAGTCACTATCACCGATAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATatca                                                                                                                                                                                                                           >  1:101743/1‑251 (MQ=255)                                               cTATCACCGATAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGatac                                                                                                                                                                                                             <  1:303041/239‑4 (MQ=255)                                                tATCACCGATAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:791165/251‑2 (MQ=255)                                                 aTCACCGATAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAg                                                                                                                                                                                                  >  1:820219/1‑248 (MQ=255)                                                 aTCACCGATAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:560341/250‑2 (MQ=255)                                                        aTAACACAACACCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAg                                                                                                                                                                                                  <  1:728572/241‑1 (MQ=255)                                                           acacaacacCATACATTTTGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGtacgtgca                                                                                                                                                                                          >  1:780066/1‑239 (MQ=255)                                                                            ttGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCtt                                                                                                                                                                                                                  <  1:498813/205‑1 (MQ=255)                                                                            ttGTGGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGtacgtgc                                                                                                                                                                                           >  1:278232/1‑222 (MQ=255)                                                                              gtgGTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGa                                                                                                                                                                  >  1:78445/1‑251 (MQ=255)                                                                                 gTACTACACTAAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACtacag                                                                                                                                                                                                        >  1:566742/1‑207 (MQ=255)                                                                                      acactaAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGtaa                                                                                                                                                          <  1:282716/249‑1 (MQ=255)                                                                                       cactaAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAAAGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGtaat                                                                                                                                                         <  1:228663/250‑1 (MQ=255)                                                                                         ctaAAAACTCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAg                                                                                                                                                                                                  <  1:220775/208‑1 (MQ=255)                                                                                                 tCGTTCGACAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:691729/202‑2 (MQ=255)                                                                                                        aCAATAGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACtacag                                                                                                                                                                                                        <  1:511697/187‑4 (MQ=255)                                                                                                             aGTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCACGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                >  1:782614/1‑189 (MQ=255)                                                                                                              gTCTTTATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:876917/189‑2 (MQ=255)                                                                                                                  ttATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                              <  1:625355/251‑1 (MQ=255)                                                                                                                  ttATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                              <  1:408840/251‑1 (MQ=255)                                                                                                                  ttATATTAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                              <  1:324068/251‑1 (MQ=255)                                                                                                                    atattAGTTTCATGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGc                                                                                                                                                                                                                    <  1:509928/160‑1 (MQ=255)                                                                                                                               aTGTGTGATATCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAacac                                                                                                                 <  1:486786/251‑1 (MQ=255)                                                                                                                                        atCAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCg                                                                                                                                                                                <  1:839672/179‑1 (MQ=255)                                                                                                                                          cAATAATAATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGc                                                                                                                                                                                                                    <  1:532605/141‑1 (MQ=255)                                                                                                                                            ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAg                                                                                                                                                                                                  >  1:765928/1‑157 (MQ=255)                                                                                                                                            ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:710279/159‑2 (MQ=255)                                                                                                                                            ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGAt                                                                                                                                                                 <  1:689380/190‑1 (MQ=255)                                                                                                                                            ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                              <  1:395769/225‑1 (MQ=255)                                                                                                                                            ataataATCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACtttt                                                                                                    >  1:272975/1‑251 (MQ=255)                                                                                                                                             taataaTCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTa                                                                                                   >  1:569616/1‑251 (MQ=255)                                                                                                                                              aataatCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACtacag                                                                                                                                                                                                        >  1:638858/1‑146 (MQ=255)                                                                                                                                               ataaTCCCTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAg                                                                                                 <  1:168698/251‑1 (MQ=255)                                                                                                                                                   tcccTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:868967/151‑2 (MQ=255)                                                                                                                                                   tcccTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATAc                                                                                                                                           >  1:342124/2‑205 (MQ=255)                                                                                                                                                   tcccTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAg                                                                                                 >  1:250263/2‑247 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCAc                                                                                                                                                                                                                                      <  1:607264/111‑1 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAg                                                                                                                                                                                                  <  1:719189/147‑1 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCAc                                                                                                                                                                                         <  1:789176/156‑1 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGta                                                                                                                                                           >  1:565482/1‑186 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAAc                                                                                                                                                      >  1:434242/1‑191 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATAcc                                                                                                                                          >  1:243357/1‑203 (MQ=255)                                                                                                                                                      cTACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTg                                                                                                                      <  1:558359/223‑1 (MQ=255)                                                                                                                                                       tACAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACaag                                                                                         <  1:799743/251‑1 (MQ=255)                                                                                                                                                        aCAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTga                                                                                                                                                                                                                                 >  1:678073/1‑113 (MQ=255)                                                                                                                                                        aCAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACaaga                                                                                        >  1:527924/1‑251 (MQ=255)                                                                                                                                                         cAGTATCTTCTGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACtacag                                                                                                                                                                                                        <  1:569384/138‑4 (MQ=255)                                                                                                                                                             atcttctGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGatac                                                                                                                                                                                                             >  1:404037/2‑126 (MQ=255)                                                                                                                                                             atcttctGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGat                                                                                                                                                                                                               <  1:68192/126‑2 (MQ=255)                                                                                                                                                               cttctGCTGAAAATGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCa                                                                                 >  1:50804/1‑251 (MQ=255)                                                                                                                                                                         aaaTGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTGCAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCt                                                                                                                                                               <  1:75352/163‑1 (MQ=255)                                                                                                                                                                          aaTGAAATCTCATCAGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCAc                                                                                                                                                                                                                       <  1:736425/106‑1 (MQ=255)                                                                                                                                                                                    catcaGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAg                                                                                                                                                                                                  <  1:422225/117‑1 (MQ=255)                                                                                                                                                                                    catcaGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGta                                                                                                                                                                                                <  1:223572/119‑2 (MQ=255)                                                                                                                                                                                    catcaGACGAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAg                                                                                                 >  1:798203/1‑214 (MQ=255)                                                                                                                                                                                         gacgAGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATAc                                                                                                                              <  1:648798/177‑1 (MQ=255)                                                                                                                                                                                             aGGTAAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAGCTTCACCTTGAATATCATCACGGCTTGATACTACAGTaa                                                                                                                                                                                                     <  1:2161/105‑1 (MQ=255)                                                                                                                                                                                                taaaaaaaTCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGa                                                <  1:289557/249‑1 (MQ=255)                                                                                                                                                                                                 aaaaaaaTCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTg                                                                                                                      >  1:208536/2‑180 (MQ=255)                                                                                                                                                                                                       aTCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGcac                                         <  1:81237/251‑1 (MQ=255)                                                                                                                                                                                                                   aaTCTCAATATTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGtc                             <  1:113693/251‑2 (MQ=255)                                                                                                                                                                                                                            atTTTCAAGACCGCGAGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACttt                                                                                                     >  1:138081/1‑170 (MQ=255)                                                                                                                                                                                                                                           aGTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCaa     >  1:649084/1‑251 (MQ=255)                                                                                                                                                                                                                                            gTACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAat    >  1:690801/1‑251 (MQ=255)                                                                                                                                                                                                                                             tACGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAata   >  1:286019/1‑251 (MQ=255)                                                                                                                                                                                                                                              aCGTAGAGTTTGTGCAACTTCACCTTGAATATCATCACGGCTTGATACTACAGTAATAGTACGTGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGCGCCGGTCTCTGCGCAATTTGAGCTACTTCAatat  <  1:170678/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AAGGATGCCCTCATCTAGTAGTTTTTGTGATAAAGAGATGGAGTCACTATCACCGATAACACAACACCATACATTTTGTGGTACTATACTAAAAACTCGTTCGACAATAGTCTTTATGTTAGTCTCATGTGTGATATCAATAATAACCCCTACAGTGTCTTCTGCTGAAAATGAAATTTCATCAGATGAGGTGAAAAAATCCTTTTTAACAATCTCAATATTTTCAAGACCGCGAGTACGTAGAATTTGTGCAACTTCACCTTGGATATCATCACGGCTTGACACTATAGTAATAGTGCGAGCACTGTCTGCCGTGATTGTTTCTTGATCTAGTAATAACATAAAAAATACCTTCTATTAATACTTCAATTGAACACGCTGATTACTTTTAAGTGCACAAGAAGTCTCATTAGAATTCCAAAAAACTACTTCTCCGGTACGATATGCACAAGGTGCCGGTTTGGGCGCAATTTGAGCTACTTCAATAT  >  1/857399‑857886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑