Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	857,222	T→C	intergenic (‑7565/‑64996)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	857,222	0	T	C	100.0%	175.7 / NA	62	intergenic (‑7565/‑64996)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/20);  total (42/20)

TTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAGCGAGGGATATCAGCACCTAAAATGATTTTCTCAACCATCTGACTTAACTGAGTATGCGAATTAAAGTAAAGGATGCCCTCATCTAGTAGTTTTTGTGATAAAGAGATGGAGTCACTATCACCGATAACACAACAC  >  1/857031‑857465                                                                                                                                                                                                |                                                                                                                                                                                                                                                    tttttgacgttATAGATAGGTTGATCGCAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAACTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTgcgc                                                                                                                                                                                           >  1:158595/10‑250 (MQ=255)         gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                                   >  1:556451/2‑249 (MQ=255)         gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                                   >  1:711880/2‑249 (MQ=255)         gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                                   >  1:817751/2‑249 (MQ=255)         gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                                   >  1:477338/2‑249 (MQ=255)            atagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgcc                                                                                                                                                                                 >  1:7724/1‑246 (MQ=255)             tagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgccctt                                                                                                                                                                              >  1:367582/1‑245 (MQ=255)                    gTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAAc                                                                                                                                                                         <  1:252111/249‑1 (MQ=255)                        aTCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                                                                                                                                                             <  1:637905/193‑1 (MQ=255)                                          gTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTAt                                                                                                                                                 >  1:407631/1‑251 (MQ=255)                                               tttCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGACAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCtt                                                                                                                                            >  1:538659/1‑251 (MQ=255)                                                                   aGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCaa                                                                                                                                                             <  1:347677/214‑1 (MQ=255)                                                                    gCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                                  >  1:748852/1‑208 (MQ=255)                                                                       caaataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                                                                                                                                                             >  1:861778/9‑146 (MQ=255)                                                                       caaataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATAtg                                                                                                                                                                                                               >  1:154381/9‑160 (MQ=255)                                                                       caaataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTaaa                                                                                                                    >  1:21137/9‑251 (MQ=255)                                                                         aataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                                  <  1:269380/197‑1 (MQ=255)                                                                              gcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTGAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGAttt                                                                                                             >  1:117216/2‑251 (MQ=255)                                                                                            tatCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATc                                                                                                                              <  1:732294/218‑1 (MQ=255)                                                                                               cTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACtt                                                                                            >  1:749870/1‑251 (MQ=255)                                                                                               cTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACtt                                                                                            >  1:589932/1‑251 (MQ=255)                                                                                                        gagcatatTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTaattgggta                                                                                   >  1:594035/5‑244 (MQ=255)                                                                                                                 cAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                                  >  1:428216/1‑163 (MQ=255)                                                                                                                      aCATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATc                                                                                                                              >  1:878548/1‑194 (MQ=255)                                                                                                                       cATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAg                                                                                                                                                    >  1:543163/1‑171 (MQ=255)                                                                                                                       cATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTaaac                                                                                                                                        >  1:701004/1‑181 (MQ=255)                                                                                                                       cATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATtaaagtaa                                                                    >  1:151701/1‑251 (MQ=255)                                                                                                                         ttACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCa                                                                                                                             <  1:155930/192‑1 (MQ=255)                                                                                                                           aCCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgcc                                                                                                                                                                                 >  1:824408/1‑135 (MQ=255)                                                                                                                            ccTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGAt                                                               >  1:596392/1‑251 (MQ=255)                                                                                                                                   gCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACtt                                                                                                                                                                       >  1:247404/1‑140 (MQ=255)                                                                                                                                      ttttATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCt                                                     <  1:56234/251‑1 (MQ=255)                                                                                                                                          aTCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCtagta                                                 <  1:36051/251‑1 (MQ=255)                                                                                                                                                taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                                  >  1:694739/1‑132 (MQ=255)                                                                                                                                                taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACt                                                                                             <  1:890194/201‑1 (MQ=255)                                                                                                                                                taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGttttt                                           >  1:329567/1‑251 (MQ=255)                                                                                                                                                taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGttttt                                           >  1:401956/1‑251 (MQ=255)                                                                                                                                                      atctaaatccTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTAtctc                                                                                                                                              <  1:712244/146‑1 (MQ=255)                                                                                                                                                      atctaaatccTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATa                                     >  1:252126/1‑251 (MQ=255)                                                                                                                                                       tctaaatccTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACtt                                                                                            >  1:661253/1‑195 (MQ=255)                                                                                                                                                                      ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATac                                                                                                                                                                                     <  1:698921/91‑1 (MQ=255)                                                                                                                                                                      ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAAt                                                                                                                                                                    >  1:394036/1‑108 (MQ=255)                                                                                                                                                                      ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATg                                                                                                                 <  1:315162/159‑1 (MQ=255)                                                                                                                                                                      ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCa                       >  1:316874/1‑249 (MQ=255)                                                                                                                                                                       cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATg                                                                                                                 >  1:405455/1‑158 (MQ=255)                                                                                                                                                                       cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCt                                                     <  1:633212/218‑1 (MQ=255)                                                                                                                                                                       cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTa                    >  1:310795/1‑251 (MQ=255)                                                                                                                                                                       cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTa                    <  1:812155/251‑1 (MQ=255)                                                                                                                                                                        aTTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTAt                   >  1:667072/1‑251 (MQ=255)                                                                                                                                                                              accTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCt                                                     <  1:618416/210‑1 (MQ=255)                                                                                                                                                                              accTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCg              <  1:666915/249‑1 (MQ=255)                                                                                                                                                                                cTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTc                        >  1:633530/1‑238 (MQ=255)                                                                                                                                                                                 ttGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATaa          >  1:330246/1‑251 (MQ=255)                                                                                                                                                                                   gggCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATaaca        >  1:522171/1‑251 (MQ=255)                                                                                                                                                                                    ggCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACAcc                                                                                                                        >  1:821264/1‑138 (MQ=255)                                                                                                                                                                                    ggCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGAt                                                               <  1:577487/195‑1 (MQ=255)                                                                                                                                                                                    ggCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATaaca        >  1:57832/1‑250 (MQ=255)                                                                                                                                                                                    ggCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATaaca        >  1:359564/1‑250 (MQ=255)                                                                                                                                                                                     gCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTa                    <  1:381331/237‑1 (MQ=255)                                                                                                                                                                                     gCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATaacac       <  1:223679/250‑1 (MQ=255)                                                                                                                                                                                        aaTAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACTGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAag                                  >  1:710296/1‑220 (MQ=255)                                                                                                                                                                                         aTAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTATCACCGATaacacaacac  <  1:811641/251‑1 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                                    TTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAGCGAGGGATATCAGCACCTAAAATGATTTTCTCAACCATCTGACTTAACTGAGTATGCGAATTAAAGTAAAGGATGCCCTCATCTAGTAGTTTTTGTGATAAAGAGATGGAGTCACTATCACCGATAACACAACAC  >  1/857031‑857465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑