Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	857,204	G→A	intergenic (‑7547/‑65014)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	857,204	0	G	A	100.0%	129.2 / NA	50	intergenic (‑7547/‑65014)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/15);  total (35/15)

TTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAGCGAGGGATATCAGCACCTAAAATGATTTTCTCAACCATCTGACTTAACTGAGTATGCGAATTAAAGTAAAGGATGCCCTCATCTAGTAGTTTTTGTGATAAAGAGATGGAGTCACTAT  >  1/856969‑857448                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     tttttctttctACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGc                                                                                                                                                                                                                                       <  1:496521/248‑1 (MQ=255)                                                               tttttgacgttATAGATAGGTTGATCGCAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAACTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTgcgc                                                                                                                                                                          >  1:158595/10‑250 (MQ=255)                                                                       gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                  >  1:556451/2‑249 (MQ=255)                                                                       gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                  >  1:711880/2‑249 (MQ=255)                                                                       gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                  >  1:477338/2‑249 (MQ=255)                                                                       gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                                                                                                                  >  1:817751/2‑249 (MQ=255)                                                                          atagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgcc                                                                                                                                                                >  1:7724/1‑246 (MQ=255)                                                                           tagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgccctt                                                                                                                                                             >  1:367582/1‑245 (MQ=255)                                                                                  gTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAAc                                                                                                                                                        <  1:252111/249‑1 (MQ=255)                                                                                      aTCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                                                                                                                                            <  1:637905/193‑1 (MQ=255)                                                                                                        gTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTAt                                                                                                                                >  1:407631/1‑251 (MQ=255)                                                                                                             tttCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGACAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCtt                                                                                                                           >  1:538659/1‑251 (MQ=255)                                                                                                                                 aGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCaa                                                                                                                                            <  1:347677/214‑1 (MQ=255)                                                                                                                                  gCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                 >  1:748852/1‑208 (MQ=255)                                                                                                                                     caaataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                                                                                                                                            >  1:861778/9‑146 (MQ=255)                                                                                                                                     caaataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATAtg                                                                                                                                                                                              >  1:154381/9‑160 (MQ=255)                                                                                                                                     caaataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTaaa                                                                                                   >  1:21137/9‑251 (MQ=255)                                                                                                                                       aataagcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                 <  1:269380/197‑1 (MQ=255)                                                                                                                                            gcTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTGAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGAttt                                                                                            >  1:117216/2‑251 (MQ=255)                                                                                                                                                          tatCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATc                                                                                                             <  1:732294/218‑1 (MQ=255)                                                                                                                                                             cTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACtt                                                                           >  1:749870/1‑251 (MQ=255)                                                                                                                                                             cTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACtt                                                                           >  1:589932/1‑251 (MQ=255)                                                                                                                                                                      gagcatatTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTaattgggta                                                                  >  1:594035/5‑244 (MQ=255)                                                                                                                                                                               cAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                 >  1:428216/1‑163 (MQ=255)                                                                                                                                                                                    aCATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATc                                                                                                             >  1:878548/1‑194 (MQ=255)                                                                                                                                                                                     cATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAg                                                                                                                                   >  1:543163/1‑171 (MQ=255)                                                                                                                                                                                     cATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTaaac                                                                                                                       >  1:701004/1‑181 (MQ=255)                                                                                                                                                                                     cATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATtaaagtaa                                                   >  1:151701/1‑251 (MQ=255)                                                                                                                                                                                       ttACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCa                                                                                                            <  1:155930/192‑1 (MQ=255)                                                                                                                                                                                         aCCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgcc                                                                                                                                                                >  1:824408/1‑135 (MQ=255)                                                                                                                                                                                          ccTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGAt                                              >  1:596392/1‑251 (MQ=255)                                                                                                                                                                                                 gCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACtt                                                                                                                                                      >  1:247404/1‑140 (MQ=255)                                                                                                                                                                                                    ttttATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCt                                    <  1:56234/251‑1 (MQ=255)                                                                                                                                                                                                        aTCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCtagta                                <  1:36051/251‑1 (MQ=255)                                                                                                                                                                                                              taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                                                                                                                 >  1:694739/1‑132 (MQ=255)                                                                                                                                                                                                              taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACt                                                                            <  1:890194/201‑1 (MQ=255)                                                                                                                                                                                                              taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGttttt                          >  1:401956/1‑251 (MQ=255)                                                                                                                                                                                                              taataaATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGttttt                          >  1:329567/1‑251 (MQ=255)                                                                                                                                                                                                                    atctaaatccTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTAtctc                                                                                                                             <  1:712244/146‑1 (MQ=255)                                                                                                                                                                                                                    atctaaatccTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATa                    >  1:252126/1‑251 (MQ=255)                                                                                                                                                                                                                     tctaaatccTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACtt                                                                           >  1:661253/1‑195 (MQ=255)                                                                                                                                                                                                                                    ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATac                                                                                                                                                                    <  1:698921/91‑1 (MQ=255)                                                                                                                                                                                                                                    ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAAt                                                                                                                                                   >  1:394036/1‑108 (MQ=255)                                                                                                                                                                                                                                    ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATg                                                                                                <  1:315162/159‑1 (MQ=255)                                                                                                                                                                                                                                    ccATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCa      >  1:316874/1‑249 (MQ=255)                                                                                                                                                                                                                                     cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATg                                                                                                >  1:405455/1‑158 (MQ=255)                                                                                                                                                                                                                                     cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCt                                    <  1:633212/218‑1 (MQ=255)                                                                                                                                                                                                                                     cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTa   >  1:310795/1‑251 (MQ=255)                                                                                                                                                                                                                                     cATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTa   <  1:812155/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGATTTTCTCAACCATCTGACTTAATTGGGTATGCGAATTAAAGTAAAGGATACCCTCATCTAGTAGTTTTTGTGATAAAGAGATTGAGTCACTAt  >  1:667072/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     TTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAGCGAGGGATATCAGCACCTAAAATGATTTTCTCAACCATCTGACTTAACTGAGTATGCGAATTAAAGTAAAGGATGCCCTCATCTAGTAGTTTTTGTGATAAAGAGATGGAGTCACTAT  >  1/856969‑857448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑