Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	857,123	G→A	intergenic (‑7466/‑65095)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	857,123	0	G	A	100.0%	78.7 / NA	31	intergenic (‑7466/‑65095)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/11);  total (20/11)

TTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAA‑TATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAGCGAGGGATATCAGCACCTAAAATGATTT  >  1/856897‑857358                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCTGTTAAA‑TATATCTAAATTAGGAGCATATTCAATa                                                                                                                                                                                                                     <  1:206159/251‑1 (MQ=255) ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAAtg                                                                                                                                                                                                                     <  1:206128/251‑2 (MQ=255)                tactgcgaatacgcTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGc                                                                                                                                                                                                      <  1:504066/240‑1 (MQ=255)                             ctcacATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGCTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAaata                                                                                                                                                                                         >  1:26506/5‑251 (MQ=255)                                       tCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAAtaaatctaa                                                                                                                                                                               >  1:32018/1‑251 (MQ=255)                                                                         tttttctttctACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGc                                                                                                                                             <  1:496521/248‑1 (MQ=255)                                                                                                   ttattatAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGAt                                                                                                                                                                                                         >  1:573624/2‑165 (MQ=255)                                                                                                                                       tttttgacgttATAGATAGGTTGATCGCAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAACTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTgcgc                                                                                >  1:158595/10‑250 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATTATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATc                                                                                                                                                                                              >  1:467729/2‑133 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAAtt                                                                                                                                                                                                                                     >  1:261018/2‑93 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAgc                                                                                                                                                                                                                               <  1:306341/98‑2 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGc                                                                                                                                                                                                      >  1:634047/2‑124 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                        >  1:711880/2‑249 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                        >  1:477338/2‑249 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                        >  1:556451/2‑249 (MQ=255)                                                                                                                                               gttATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccg                                                                        >  1:817751/2‑249 (MQ=255)                                                                                                                                                  atagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgcc                                                                      >  1:7724/1‑246 (MQ=255)                                                                                                                                                   tagatagGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATaccgccctt                                                                   >  1:367582/1‑245 (MQ=255)                                                                                                                                                          gTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAAc                                                              <  1:252111/249‑1 (MQ=255)                                                                                                                                                              aTCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTa                                                                                                                                                               <  1:470018/148‑1 (MQ=255)                                                                                                                                                              aTCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                                                  <  1:637905/193‑1 (MQ=255)                                                                                                                                                                                gTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTAt                                      >  1:407631/1‑251 (MQ=255)                                                                                                                                                                                     tttCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGACAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCtt                                 >  1:538659/1‑251 (MQ=255)                                                                                                                                                                                                         aGCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCaa                                                  <  1:347677/214‑1 (MQ=255)                                                                                                                                                                                                          gCTCAAATAAGCTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                       >  1:748852/1‑208 (MQ=255)                                                                                                                                                                                                             caaataagcTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAAtc                                                                                                                  >  1:861778/9‑146 (MQ=255)                                                                                                                                                                                                             caaataagcTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATAtg                                                                                                    >  1:154381/9‑160 (MQ=255)                                                                                                                                                                                                             caaataagcTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTaaa         >  1:21137/9‑251 (MQ=255)                                                                                                                                                                                                               aataagcTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCGCCCTTACAACTTAATAc                                                       <  1:269380/197‑1 (MQ=255)                                                                                                                                                                                                                    gcTCCCGTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGCACCGGAATTTTTTTACTTGATACAATCTCATTAGCAATATGTGAACTAATTGAACTTGCGCCAATACCGCCCTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAACGAGGTATATCAACACCTAAAATGAttt  >  1:117216/2‑251 (MQ=255)                                                                                                                                                                                                                          gTTAAA‑TATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAggac                                                                                                                                                           <  1:148247/92‑3 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAA‑TATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGCACTGGAATTTTTTTACTTGACACAATCTCATTAGCAATATGTGAACTAATTAAACTTGCGCCAATACCACCTTTACAACTTAATACAGCAATTTTAACAGTATCTCTTAAGCGAGGGATATCAGCACCTAAAATGATTT  >  1/856897‑857358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑