Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,995	C→T	intergenic (‑7338/‑65223)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,995	0	C	T	100.0%	29.8 / NA	13	intergenic (‑7338/‑65223)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/6);  total (7/6)

GTTTCTATATCATTTTTGGCCATTAACTTAGCTGATTCCATTCGATTATCAGAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGC  >  1/856824‑857219                                                                                                                                                                            |                                                                                                                                                                                                                                 gTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTa                                                                                                                                                   >  1:818262/1‑251 (MQ=255)       atatCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTAttttt                                                                                                                                              >  1:667290/1‑250 (MQ=255)       atatCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTc                                                                                                                                             <  1:790560/251‑1 (MQ=255)            attcttagCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTcg                                                                                                                                        >  1:650576/8‑251 (MQ=255)                                                    gAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAGCTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAatatat                                                                                                 >  1:860437/1‑248 (MQ=255)                                                           ataCAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAAt                                                                                                                                                       >  1:417066/3‑189 (MQ=255)                                                                          ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCTGTTAAATATATCTAAATTAGGAGCATATTCAATa                                                                          <  1:206159/251‑1 (MQ=255)                                                                          ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAAtg                                                                          <  1:206128/251‑2 (MQ=255)                                                                                         tactgcgaatacgcTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGc                                                           <  1:504066/240‑1 (MQ=255)                                                                                                      ctcacATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGCTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAaata                                              >  1:26506/5‑251 (MQ=255)                                                                                                                tCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAAtaaatctaa                                    >  1:32018/1‑251 (MQ=255)                                                                                                                                               cgatttttctttctACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAata                                                                                                    <  1:424188/150‑1 (MQ=255)                                                                                                                                                  tttttctttctACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCCTGGGAACCATTAGGACCTTGGGCTAATAGc  <  1:496521/248‑1 (MQ=255)                                                                                                                                                                            |                                                                                                                                                                                                                                 GTTTCTATATCATTTTTGGCCATTAACTTAGCTGATTCCATTCGATTATCAGAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAAATCTTGAGAACCATTAGGGCCTTGGGCTAATAGC  >  1/856824‑857219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑