Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,946	C→T	intergenic (‑7289/‑65272)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,946	0	C	T	100.0%	24.6 / NA	11	intergenic (‑7289/‑65272)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/4);  total (7/4)

GTTTCTATATCATTTTTGGCCATTAACTTAGCTGATTCCATTCGATTATCAGAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAA  >  1/856824‑857185                                                                                                                           |                                                                                                                                                                                                                                                gTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTa                                                                                                                 >  1:818262/1‑251 (MQ=255)       atatCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTAttttt                                                                                                            >  1:667290/1‑250 (MQ=255)       atatCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTc                                                                                                           <  1:790560/251‑1 (MQ=255)            attcttagCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTcg                                                                                                      >  1:650576/8‑251 (MQ=255)                                                    gAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAGCTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAatatat                                                               >  1:860437/1‑248 (MQ=255)                                                           ataCAAAAGTGCGAATTGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAAt                                                                                                                     >  1:417066/3‑189 (MQ=255)                                                                          ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCTGTTAAATATATCTAAATTAGGAGCATATTCAATa                                        <  1:206159/251‑1 (MQ=255)                                                                          ttGGTTTTCTCGAAGTACTGCGAATACGCTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAAtg                                        <  1:206128/251‑2 (MQ=255)                                                                                         tactgcgaatacgcTCACATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGc                         <  1:504066/240‑1 (MQ=255)                                                                                                      ctcacATTCATCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGCTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAaata            >  1:26506/5‑251 (MQ=255)                                                                                                                tCTAGAAACTGTTTAGCTAAACGTAGAGAACCGATTTTTCTTTCTACAACTAATACGAAATTATTATAGTTTTCTAAAAAACTAATGAAATTATCCTTTTTGACGTTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCTGCGGGTGTTAGCTCAAATAAGCTCCCGTTAAATATATCTAAATTAGGAGCATATTCAATAACATTACCTGATAGCTTTTTATCAAATAAtaaatctaa  >  1:32018/1‑251 (MQ=255)                                                                                                                           |                                                                                                                                                                                                                                                GTTTCTATATCATTTTTGGCCATTAACTTAGCTGATTCCATTCGATTATCAGAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGATGCGCTCGCATTCATCTAGGAACTGCTTAGCTAAACGTAGCGAACCAATTCTTCTTTCTACAACTAATACGAAACTATTATAGTTTTCTAAAAAACCAATGAAATTATCCTTCTTAACATTATAGATAGGTTGATCGTAGATAATAAAATTGTATTTTTCAGTCGCCGCAGGTGTTAGCTCAAATAGACTCCCGTTAAATATGTCTAAATTCGGAGTATATTCAATAACATTACCTGATAGCTTTTTATCAAATAATAAATCTAA  >  1/856824‑857185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑