Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,754	A→G	intergenic (‑7097/‑65464)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,754	0	A	G	100.0%	62.7 / NA	23	intergenic (‑7097/‑65464)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

TTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGAGTTTTGAAAAAAATGAAGAAAATAAACTCTGTTTTTCTTTCGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAATGAATTAATTTCCTTTTTACCATCACGACCTAAGTTAATACCTAATATGGTTTTTGTATTAGTGTTTTTAATATACGGGATTACGGCATCTATTGAAGAACCAATTAAAGTTTCTATATCATTTTTGGCCATTAACTTAGCTGATTCCATTCGATTATCAGAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGAT  >  1/856512‑856921                                                                                                                                                                                                                                                   |                                                                                                                                                                        ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                                                                                                                                                                 <  1:116083/251‑2 (MQ=255) ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                                                                                                                                                                 <  1:678648/251‑2 (MQ=255) ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                                                                                                                                                                 <  1:515766/251‑2 (MQ=255) ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                                                                                                                                                                 <  1:484265/251‑2 (MQ=255)   cttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatacag                                                                                                                                                               <  1:345357/251‑4 (MQ=255)                         tCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTaac                                                                                                                                         <  1:106686/251‑2 (MQ=255)                                            gctcaatACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTaac                                                                                                                                         >  1:476089/7‑231 (MQ=255)                                                   acttcacttcTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGCTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTgaag                                                                                                               <  1:577410/251‑1 (MQ=255)                                                      tcacttcTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAAc                                                                                                            <  1:44448/251‑1 (MQ=255)                                                                    gATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAAc                                                                                                            >  1:402244/1‑237 (MQ=255)                                                                                          aTGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTaa                                                                          >  1:256312/1‑249 (MQ=255)                                                                                           tGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACtt                                                                       >  1:591750/1‑251 (MQ=255)                                                                                                       acttattGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTctaa                                                           >  1:231075/7‑248 (MQ=255)                                                                                                              gTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTctaaccgatta                                                    >  1:506710/1‑241 (MQ=255)                                                                                                              gTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTc                                                              <  1:847208/241‑1 (MQ=255)                                                                                                                  aaTGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAg                                                <  1:171135/251‑1 (MQ=255)                                                                                                                            gtaaaaaaTGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGt                                                                                                                                                        >  1:843045/3‑137 (MQ=255)                                                                                                                                                       ttttCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGt           <  1:63248/251‑1 (MQ=255)                                                                                                                                                                ggCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACATACAAAAGTGCGAATTGGTTTTCTCGAAGtactgcgaat  >  1:883817/1‑242 (MQ=255)                                                                                                                                                                 gCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTgaag                                                                                                               <  1:184587/141‑1 (MQ=255)                                                                                                                                                                 gCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACatac                                      >  1:858971/1‑211 (MQ=255)                                                                                                                                                                                atatTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTaagg                                                                                                   >  1:393820/1‑136 (MQ=255)                                                                                                                                                                                atatTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTAACAAAAGGAATTATGGCATCTATTGAAGAACCGATTAAGGTTTCGATATCATTCTTAGCCATTAACTTAGCTGCTTCTAACCGATTATCAGAAATACat                                        >  1:19406/1‑196 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                        TTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGAGTTTTGAAAAAAATGAAGAAAATAAACTCTGTTTTTCTTTCGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAATGAATTAATTTCCTTTTTACCATCACGACCTAAGTTAATACCTAATATGGTTTTTGTATTAGTGTTTTTAATATACGGGATTACGGCATCTATTGAAGAACCAATTAAAGTTTCTATATCATTTTTGGCCATTAACTTAGCTGATTCCATTCGATTATCAGAAATACAGACAAAAGTACGAATTGGTTTTCTCGAAGTGCCACGGAT  >  1/856512‑856921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑