Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,560	G→A	intergenic (‑6903/‑65658)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,560	0	G	A	100.0%	138.4 / NA	52	intergenic (‑6903/‑65658)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

TCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGAGTTTTGAAAAAAATGAAGAAAATAAACTCTGTTTTTCTTTCGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAATGAATTAATTTCCTTTTTACCATCACGACCTAAGTTAATACCTAATATGGTTTTTGTATTAGTGTTTTTAAT  >  1/856325‑856786                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATacttcac                                                                                                                                                                                                                            >  1:152822/1‑244 (MQ=255) tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCtaag                                                                                                                                                                                                                     >  1:840605/1‑251 (MQ=255)               aaCTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTttgtt                                                                                                                                                                                                       >  1:536723/1‑251 (MQ=255)                      atatCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGt                                                                                                                                                                                                >  1:8576/1‑251 (MQ=255)                          cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTATTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                                                                                                                                                                             >  1:184408/1‑250 (MQ=255)                          cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                                                                                                                                                                             >  1:764746/1‑250 (MQ=255)                          cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                                                                                                                                                                             >  1:328925/1‑250 (MQ=255)                            tttATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCAt                                                                                                                                                                                          >  1:722872/1‑251 (MQ=255)                                    aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:42600/251‑4 (MQ=255)                                    aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:367179/251‑4 (MQ=255)                                    aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaac                                                                                                                                                                                   >  1:167461/1‑248 (MQ=255)                                         cAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTa                                                                                                                                                                              >  1:551862/1‑250 (MQ=255)                                               cgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg                                                                                                                                                                       <  1:123341/250‑4 (MQ=255)                                               cgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg                                                                                                                                                                       <  1:386819/250‑4 (MQ=255)                                                       cGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa                                                                                                                                                               <  1:646085/251‑1 (MQ=255)                                                                ccATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:879319/223‑4 (MQ=255)                                                                ccATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTgtaa                                                                                                                                                      >  1:61077/1‑248 (MQ=255)                                                                     aCCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:612660/218‑4 (MQ=255)                                                                        cTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa                                                                                                                                                               >  1:391690/1‑234 (MQ=255)                                                                          cAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCa                                                                                                                                                                                 <  1:330389/214‑1 (MQ=255)                                                                            aTCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  >  1:71867/1‑208 (MQ=255)                                                                                  tcaGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAAc                                                                                                                                    <  1:62286/251‑1 (MQ=255)                                                                                           aTTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGAGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGttttt                                                                                                                           >  1:73968/1‑251 (MQ=255)                                                                                                                   gatgacgtcaaataagtgttacTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGat                                                                                                    <  1:500580/229‑1 (MQ=255)                                                                                                                       acgtcaaataagtgttacTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTg                                                                                                >  1:214262/18‑250 (MQ=255)                                                                                                                                aagtgttacTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAAc                                                                                      <  1:61538/243‑1 (MQ=255)                                                                                                                                        cTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATg                                                                                                                                                                >  1:452402/1‑169 (MQ=255)                                                                                                                                              ttGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTagtg                                                                        <  1:739751/251‑4 (MQ=255)                                                                                                                                                  caatacgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGaatt                                                                    >  1:763224/9‑251 (MQ=255)                                                                                                                                                     tacgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa                                                                                                                                                               >  1:45016/6‑157 (MQ=255)                                                                                                                                                      acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTttgt                                                                                                                                                                                                        >  1:52017/5‑115 (MQ=255)                                                                                                                                                      acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTc                                                                                                                                                                                               >  1:58/5‑124 (MQ=255)                                                                                                                                                      acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGat                                                                                                    <  1:65563/211‑1 (MQ=255)                                                                                                                                                      acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAAc                                                                                      >  1:466703/5‑229 (MQ=255)                                                                                                                                                      acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGaattaatt                                                                <  1:175824/247‑1 (MQ=255)                                                                                                                                                        gattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATg                                                                                                                                                                                         <  1:414397/126‑1 (MQ=255)                                                                                                                                                               aaTCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGt                                                                                                                                                                      >  1:628842/1‑140 (MQ=255)                                                                                                                                                                aTCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCtttttc                                                       >  1:539729/1‑249 (MQ=255)                                                                                                                                                                     aaCCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTgtaa                                                                                                                                                      >  1:488547/1‑147 (MQ=255)                                                                                                                                                                                ttactaatGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGtt                                      <  1:328079/244‑1 (MQ=255)                                                                                                                                                                                   ctaatGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg                                                                                                                                                                       >  1:244210/5‑116 (MQ=255)                                                                                                                                                                                    taatGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGGTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAAtg                                  <  1:718472/248‑2 (MQ=255)                                                                                                                                                                                          gCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCtaagt                                                                                                                                                                                                                    <  1:513837/67‑1 (MQ=255)                                                                                                                                                                                            ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:515766/251‑2 (MQ=255)                                                                                                                                                                                            ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:678648/251‑2 (MQ=255)                                                                                                                                                                                            ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:484265/251‑2 (MQ=255)                                                                                                                                                                                            ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:116083/251‑2 (MQ=255)                                                                                                                                                                                              cttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatacag                        <  1:345357/251‑4 (MQ=255)                                                                                                                                                                                                                    tCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTaac  <  1:106686/251‑2 (MQ=255)                                                                                                                                                                                                                            acatCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAAt                                                                                                                                        >  1:575132/3‑109 (MQ=255)                                                                                                                                                                                                                             catCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAAcc                                                                                     >  1:412526/2‑159 (MQ=255)                                                                                                                                                                                                                             catCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAAtgc                                 >  1:529389/2‑209 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   TCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGAGTTTTGAAAAAAATGAAGAAAATAAACTCTGTTTTTCTTTCGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAATGAATTAATTTCCTTTTTACCATCACGACCTAAGTTAATACCTAATATGGTTTTTGTATTAGTGTTTTTAAT  >  1/856325‑856786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑