Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,545	T→C	intergenic (‑6888/‑65673)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,545	0	T	C	100.0%	179.3 / NA	62	intergenic (‑6888/‑65673)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/29);  total (33/29)

GAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGAGTTTTGAAAAAAATGAAGAAAATAAACTCTGTTTTTCTTTCGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAATGAATTAATTTCCTTTTTACCATCACGACCTAAGTTAATACCTAATATGGTTTTTGTATTAGTGTTTTTAAT  >  1/856299‑856786                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCa                                                                                                                                                                                                                                               >  1:211039/1‑251 (MQ=255)             gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                                                                                                                                                                                                   >  1:860441/1‑244 (MQ=255)             gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                                                                                                                                                                                                   >  1:177570/1‑244 (MQ=255)             gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGCCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                                                                                                                                                                                                   >  1:36229/1‑244 (MQ=255)               tCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaatac                                                                                                                                                                                                                                 <  1:590920/251‑10 (MQ=255)               tCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaatac                                                                                                                                                                                                                                 <  1:676058/251‑10 (MQ=255)                  aCCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctc                                                                                                                                                                                                                                      <  1:188727/243‑5 (MQ=255)                           tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATacttcac                                                                                                                                                                                                                            >  1:152822/1‑244 (MQ=255)                           tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCtaag                                                                                                                                                                                                                     >  1:840605/1‑251 (MQ=255)                            cATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                                                                                                                                                                                           <  1:816649/228‑1 (MQ=255)                                         aaCTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTttgtt                                                                                                                                                                                                       >  1:536723/1‑251 (MQ=255)                                                atatCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGt                                                                                                                                                                                                >  1:8576/1‑251 (MQ=255)                                                  atCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                                                                                                                                                                                           <  1:176770/206‑1 (MQ=255)                                                  atCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                                                                                                                                                                                           >  1:496865/1‑206 (MQ=255)                                                    cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTATTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                                                                                                                                                                             >  1:184408/1‑250 (MQ=255)                                                    cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                                                                                                                                                                                           >  1:221242/1‑204 (MQ=255)                                                    cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                                                                                                                                                                             >  1:764746/1‑250 (MQ=255)                                                    cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                                                                                                                                                                             >  1:328925/1‑250 (MQ=255)                                                      tttATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCAt                                                                                                                                                                                          >  1:722872/1‑251 (MQ=255)                                                              aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:367179/251‑4 (MQ=255)                                                              aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:42600/251‑4 (MQ=255)                                                              aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaac                                                                                                                                                                                   >  1:167461/1‑248 (MQ=255)                                                                   cAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTa                                                                                                                                                                              >  1:551862/1‑250 (MQ=255)                                                                         cgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg                                                                                                                                                                       <  1:386819/250‑4 (MQ=255)                                                                         cgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg                                                                                                                                                                       <  1:123341/250‑4 (MQ=255)                                                                              tcccGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                                                                                                                                                                                           >  1:198914/4‑178 (MQ=255)                                                                                 cGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa                                                                                                                                                               <  1:646085/251‑1 (MQ=255)                                                                                          ccATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:879319/223‑4 (MQ=255)                                                                                          ccATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTgtaa                                                                                                                                                      >  1:61077/1‑248 (MQ=255)                                                                                               aCCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  <  1:612660/218‑4 (MQ=255)                                                                                                  cTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa                                                                                                                                                               >  1:391690/1‑234 (MQ=255)                                                                                                    cAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCa                                                                                                                                                                                 <  1:330389/214‑1 (MQ=255)                                                                                                      aTCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                                                                                                                                                                                  >  1:71867/1‑208 (MQ=255)                                                                                                            tcaGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAAc                                                                                                                                    <  1:62286/251‑1 (MQ=255)                                                                                                                     aTTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGAGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGttttt                                                                                                                           >  1:73968/1‑251 (MQ=255)                                                                                                                                             gatgacgtcaaataagtgttacTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGat                                                                                                    <  1:500580/229‑1 (MQ=255)                                                                                                                                                 acgtcaaataagtgttacTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTg                                                                                                >  1:214262/18‑250 (MQ=255)                                                                                                                                                          aagtgttacTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAAc                                                                                      <  1:61538/243‑1 (MQ=255)                                                                                                                                                                  cTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATg                                                                                                                                                                >  1:452402/1‑169 (MQ=255)                                                                                                                                                                    tttATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                                                                                                                                                                                           >  1:511869/1‑92 (MQ=255)                                                                                                                                                                        ttGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTagtg                                                                        <  1:739751/251‑4 (MQ=255)                                                                                                                                                                            caatacgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGaatt                                                                    >  1:763224/9‑251 (MQ=255)                                                                                                                                                                               tacgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa                                                                                                                                                               >  1:45016/6‑157 (MQ=255)                                                                                                                                                                                acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTttgt                                                                                                                                                                                                        >  1:52017/5‑115 (MQ=255)                                                                                                                                                                                acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTc                                                                                                                                                                                               >  1:58/5‑124 (MQ=255)                                                                                                                                                                                acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGat                                                                                                    <  1:65563/211‑1 (MQ=255)                                                                                                                                                                                acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAAc                                                                                      >  1:466703/5‑229 (MQ=255)                                                                                                                                                                                acgattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGaattaatt                                                                <  1:175824/247‑1 (MQ=255)                                                                                                                                                                                  gattAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATg                                                                                                                                                                                         <  1:414397/126‑1 (MQ=255)                                                                                                                                                                                         aaTCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGt                                                                                                                                                                      >  1:628842/1‑140 (MQ=255)                                                                                                                                                                                          aTCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCtttttc                                                       >  1:539729/1‑249 (MQ=255)                                                                                                                                                                                               aaCCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTgtaa                                                                                                                                                      >  1:488547/1‑147 (MQ=255)                                                                                                                                                                                                          ttactaatGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGtt                                      <  1:328079/244‑1 (MQ=255)                                                                                                                                                                                                             ctaatGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg                                                                                                                                                                       >  1:244210/5‑116 (MQ=255)                                                                                                                                                                                                              taatGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGGTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAAtg                                  <  1:718472/248‑2 (MQ=255)                                                                                                                                                                                                                    gCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCtaagt                                                                                                                                                                                                                    <  1:513837/67‑1 (MQ=255)                                                                                                                                                                                                                      ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:116083/251‑2 (MQ=255)                                                                                                                                                                                                                      ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:678648/251‑2 (MQ=255)                                                                                                                                                                                                                      ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:484265/251‑2 (MQ=255)                                                                                                                                                                                                                      ttcttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatac                          <  1:515766/251‑2 (MQ=255)                                                                                                                                                                                                                        cttTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAatacag                        <  1:345357/251‑4 (MQ=255)                                                                                                                                                                                                                                              tCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGAGTTTTGTAAAAAATGAGGAAAATAAACTTTGTTTTTCTTTTGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAGTGAATTAATTTCCTTTTTCCCATCACGACCTAAGTTAATGCCCAATACAGTTTTTGTATTAGTATTTTTaac  <  1:106686/251‑2 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGAGTTTTGAAAAAAATGAAGAAAATAAACTCTGTTTTTCTTTCGGCTTACTACTGCGAGATATTGCACCAATAACCTTTAACATTAATGAATTAATTTCCTTTTTACCATCACGACCTAAGTTAATACCTAATATGGTTTTTGTATTAGTGTTTTTAAT  >  1/856299‑856786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑