Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,387	T→A	intergenic (‑6730/‑65831)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,387	0	T	A	100.0%	174.6 / NA	63	intergenic (‑6730/‑65831)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/29);  total (34/29)

CGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGGCGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACAAATTCTTTATCAGTTTTTTCCAGAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGA  >  1/856144‑856629                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   cGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAc                                                                                                                                                                                                                                             <  1:698551/251‑1 (MQ=255)   gTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAcc                                                                                                                                                                                                                                            <  1:698670/250‑1 (MQ=255)        attacaacaTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAAt                                                                                                                                                                                                                                      >  1:188624/4‑251 (MQ=255)                aTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAccc                                                                                                                                                                                                                                           >  1:329677/1‑238 (MQ=255)                         gctaccATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAccc                                                                                                                                                                                                                                           >  1:594915/5‑229 (MQ=255)                                  gggTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATaa                                                                                                                                                                                                            >  1:758068/2‑251 (MQ=255)                                     tAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                                          <  1:521936/250‑2 (MQ=255)                                              gTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                                          >  1:845249/1‑240 (MQ=255)                                                         gACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCaaa                                                                                                                                                                                     >  1:865849/1‑251 (MQ=255)                                                                  catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAAtc                                                                                                                                                                                                                                     <  1:742115/194‑1 (MQ=255)                                                                  catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGcaacaa                                                                                                                                                                                                                        <  1:353882/207‑1 (MQ=255)                                                                  catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATa                                                                                                                                                                                   <  1:287448/244‑1 (MQ=255)                                                                  catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATa                                                                                                                                                                                   <  1:835611/244‑1 (MQ=255)                                                                          cgacGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAgtgttacttttatt                                                                                                                                                                    <  1:15157/248‑14 (MQ=255)                                                                                          cAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAAc                                                                                                                                                     <  1:599990/250‑1 (MQ=255)                                                                                                       tGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGtt                                                                                                                                       <  1:865880/251‑1 (MQ=255)                                                                                                             atCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTa                                                                                                                                 <  1:203429/251‑1 (MQ=255)                                                                                                              tCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCTGTAACGGACCATAACCCTCAATCTCATCAGCAACAACTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTa                                                                                                                                 >  1:91403/1‑250 (MQ=255)                                                                                                                aGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATc                                                                                                                                                 >  1:338096/1‑232 (MQ=255)                                                                                                                  ttAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTActaa                                                                                                                             <  1:719901/250‑4 (MQ=255)                                                                                                                        gTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGc                                                                                                                        <  1:378392/249‑1 (MQ=255)                                                                                                                                                            gAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCAc                                                                                                               >  1:23339/1‑222 (MQ=255)                                                                                                                                                            gAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCa                                                                                  >  1:211039/1‑251 (MQ=255)                                                                                                                                                              aTACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGAAACGGACTATAACCCTCAATCTCATCAGCAACAAttt                                                                                                                                                                                                                     >  1:184739/1‑118 (MQ=255)                                                                                                                                                                accggCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTActaa                                                                                                                             >  1:473317/5‑201 (MQ=255)                                                                                                                                                                        gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                                      >  1:860441/1‑244 (MQ=255)                                                                                                                                                                        gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                                      >  1:177570/1‑244 (MQ=255)                                                                                                                                                                        gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGCCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                                      >  1:36229/1‑244 (MQ=255)                                                                                                                                                                          tCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaatac                                                                    <  1:676058/251‑10 (MQ=255)                                                                                                                                                                          tCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaatac                                                                    <  1:590920/251‑10 (MQ=255)                                                                                                                                                                             aCCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctc                                                                         <  1:188727/243‑5 (MQ=255)                                                                                                                                                                                cAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGc                                                                                                                        >  1:645237/1‑193 (MQ=255)                                                                                                                                                                                  gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAAtt                                                                                                                                                                                                                      >  1:54687/5‑97 (MQ=255)                                                                                                                                                                                  gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAccagtgc                                                                                                                                                                                                     >  1:415348/5‑108 (MQ=255)                                                                                                                                                                                  gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                                          >  1:1732/5‑108 (MQ=255)                                                                                                                                                                                  gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCAc                                                                                                               <  1:264397/196‑1 (MQ=255)                                                                                                                                                                                      tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAAc                                                                                                                                                     <  1:80875/158‑1 (MQ=255)                                                                                                                                                                                      tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATacttcac                                                               >  1:152822/1‑244 (MQ=255)                                                                                                                                                                                      tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCtaag                                                        >  1:840605/1‑251 (MQ=255)                                                                                                                                                                                       cATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAAc                                                                                                                                                     <  1:282767/157‑1 (MQ=255)                                                                                                                                                                                       cATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                              <  1:816649/228‑1 (MQ=255)                                                                                                                                                                                              gCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATc                                                                                                                                                 >  1:547307/1‑154 (MQ=255)                                                                                                                                                                                                    aaCTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACtt                                                                                                             <  1:308875/184‑1 (MQ=255)                                                                                                                                                                                                    aaCTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTttgtt                                          >  1:536723/1‑251 (MQ=255)                                                                                                                                                                                                           atatCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGt                                   >  1:8576/1‑251 (MQ=255)                                                                                                                                                                                                             atCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                              >  1:496865/1‑206 (MQ=255)                                                                                                                                                                                                             atCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                              <  1:176770/206‑1 (MQ=255)                                                                                                                                                                                                               cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                                          <  1:724359/80‑2 (MQ=255)                                                                                                                                                                                                               cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTATTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                >  1:184408/1‑250 (MQ=255)                                                                                                                                                                                                               cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                              >  1:221242/1‑204 (MQ=255)                                                                                                                                                                                                               cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                >  1:328925/1‑250 (MQ=255)                                                                                                                                                                                                               cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                                >  1:764746/1‑250 (MQ=255)                                                                                                                                                                                                                 tttATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCAt                             >  1:722872/1‑251 (MQ=255)                                                                                                                                                                                                                         aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                     <  1:42600/251‑4 (MQ=255)                                                                                                                                                                                                                         aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc                     <  1:367179/251‑4 (MQ=255)                                                                                                                                                                                                                         aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaac                      >  1:167461/1‑248 (MQ=255)                                                                                                                                                                                                                            gTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAgtg                                                                                                                                                                               <  1:683125/94‑3 (MQ=255)                                                                                                                                                                                                                              cAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTa                 >  1:551862/1‑250 (MQ=255)                                                                                                                                                                                                                                  cacgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGCGCATCGGATGACGTCAAATAAgtg                                                                                                                                                                               >  1:205922/4‑86 (MQ=255)                                                                                                                                                                                                                                    cgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg          <  1:123341/250‑4 (MQ=255)                                                                                                                                                                                                                                    cgagaTCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTattg          <  1:386819/250‑4 (MQ=255)                                                                                                                                                                                                                                         tcccGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                                              >  1:198914/4‑178 (MQ=255)                                                                                                                                                                                                                                            cGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTACTTATTGTTTAATGa  <  1:646085/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGGCGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACAAATTCTTTATCAGTTTTTTCCAGAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTATTTACTGTTTAATGA  >  1/856144‑856629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑