Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,371	T→C	intergenic (‑6714/‑65847)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,371	0	T	C	100.0%	176.7 / NA	62	intergenic (‑6714/‑65847)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/27);  total (35/27)

ATCTAATGCAATCGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGGCGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACAAATTCTTTATCAGTTTTTTCCAGAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTA  >  1/856132‑856614                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    aTCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTa                                                                                                                                                                                                                                          >  1:705393/1‑251 (MQ=255) aTCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTa                                                                                                                                                                                                                                          >  1:823350/1‑251 (MQ=255)       cgCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTaacggac                                                                                                                                                                                                                                    <  1:41424/250‑6 (MQ=255)             cGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAc                                                                                                                                                                                                                              <  1:698551/251‑1 (MQ=255)               gTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAcc                                                                                                                                                                                                                             <  1:698670/250‑1 (MQ=255)                    attacaacaTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAAt                                                                                                                                                                                                                       >  1:188624/4‑251 (MQ=255)                            aTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAccc                                                                                                                                                                                                                            >  1:329677/1‑238 (MQ=255)                                     gctaccATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAccc                                                                                                                                                                                                                            >  1:594915/5‑229 (MQ=255)                                              gggTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATaa                                                                                                                                                                                             >  1:758068/2‑251 (MQ=255)                                                 tAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                           <  1:521936/250‑2 (MQ=255)                                                          gTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGAt                                                                                                                                                                                                                                                >  1:630038/1‑188 (MQ=255)                                                          gTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                           >  1:845249/1‑240 (MQ=255)                                                                     gACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCaaa                                                                                                                                                                      >  1:865849/1‑251 (MQ=255)                                                                              catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAAtc                                                                                                                                                                                                                      <  1:742115/194‑1 (MQ=255)                                                                              catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGcaacaa                                                                                                                                                                                                         <  1:353882/207‑1 (MQ=255)                                                                              catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATa                                                                                                                                                                    <  1:287448/244‑1 (MQ=255)                                                                              catcaATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATa                                                                                                                                                                    <  1:835611/244‑1 (MQ=255)                                                                                      cgacGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAgtgttacttttatt                                                                                                                                                     <  1:15157/248‑14 (MQ=255)                                                                                                      cAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAAc                                                                                                                                      <  1:599990/250‑1 (MQ=255)                                                                                                                   tGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGtt                                                                                                                        <  1:865880/251‑1 (MQ=255)                                                                                                                         atCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTa                                                                                                                  <  1:203429/251‑1 (MQ=255)                                                                                                                          tCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCTGTAACGGACCATAACCCTCAATCTCATCAGCAACAACTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTa                                                                                                                  >  1:91403/1‑250 (MQ=255)                                                                                                                            aGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATc                                                                                                                                  >  1:338096/1‑232 (MQ=255)                                                                                                                              ttAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTActaa                                                                                                              <  1:719901/250‑4 (MQ=255)                                                                                                                                    gTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGc                                                                                                         <  1:378392/249‑1 (MQ=255)                                                                                                                                                                        gAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCAc                                                                                                >  1:23339/1‑222 (MQ=255)                                                                                                                                                                        gAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCa                                                                   >  1:211039/1‑251 (MQ=255)                                                                                                                                                                          aTACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGAAACGGACTATAACCCTCAATCTCATCAGCAACAAttt                                                                                                                                                                                                      >  1:184739/1‑118 (MQ=255)                                                                                                                                                                            accggCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTActaa                                                                                                              >  1:473317/5‑201 (MQ=255)                                                                                                                                                                                    gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                       >  1:860441/1‑244 (MQ=255)                                                                                                                                                                                    gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                       >  1:177570/1‑244 (MQ=255)                                                                                                                                                                                    gTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGCCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaat                                                       >  1:36229/1‑244 (MQ=255)                                                                                                                                                                                      tCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaatac                                                     <  1:590920/251‑10 (MQ=255)                                                                                                                                                                                      tCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctcaatac                                                     <  1:676058/251‑10 (MQ=255)                                                                                                                                                                                         aCCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAttgctc                                                          <  1:188727/243‑5 (MQ=255)                                                                                                                                                                                            cAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGc                                                                                                         >  1:645237/1‑193 (MQ=255)                                                                                                                                                                                              gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAAtt                                                                                                                                                                                                       >  1:54687/5‑97 (MQ=255)                                                                                                                                                                                              gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAccagtgc                                                                                                                                                                                      >  1:415348/5‑108 (MQ=255)                                                                                                                                                                                              gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                           >  1:1732/5‑108 (MQ=255)                                                                                                                                                                                              gatatCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCAc                                                                                                <  1:264397/196‑1 (MQ=255)                                                                                                                                                                                                  tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAAc                                                                                                                                      <  1:80875/158‑1 (MQ=255)                                                                                                                                                                                                  tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATacttcac                                                >  1:152822/1‑244 (MQ=255)                                                                                                                                                                                                  tCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCtaag                                         >  1:840605/1‑251 (MQ=255)                                                                                                                                                                                                   cATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAAc                                                                                                                                      <  1:282767/157‑1 (MQ=255)                                                                                                                                                                                                   cATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                               <  1:816649/228‑1 (MQ=255)                                                                                                                                                                                                          gCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATc                                                                                                                                  >  1:547307/1‑154 (MQ=255)                                                                                                                                                                                                                aaCTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACtt                                                                                              <  1:308875/184‑1 (MQ=255)                                                                                                                                                                                                                aaCTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTttgtt                           >  1:536723/1‑251 (MQ=255)                                                                                                                                                                                                                       atatCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGt                    >  1:8576/1‑251 (MQ=255)                                                                                                                                                                                                                         atCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                               <  1:176770/206‑1 (MQ=255)                                                                                                                                                                                                                         atCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                               >  1:496865/1‑206 (MQ=255)                                                                                                                                                                                                                           cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAAcc                                                                                                                                                                                           <  1:724359/80‑2 (MQ=255)                                                                                                                                                                                                                           cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTATTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                 >  1:184408/1‑250 (MQ=255)                                                                                                                                                                                                                           cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGAt                                                               >  1:221242/1‑204 (MQ=255)                                                                                                                                                                                                                           cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                 >  1:328925/1‑250 (MQ=255)                                                                                                                                                                                                                           cATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAg                 >  1:764746/1‑250 (MQ=255)                                                                                                                                                                                                                             tttATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCAt              >  1:722872/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc      <  1:367179/251‑4 (MQ=255)                                                                                                                                                                                                                                     aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaacc      <  1:42600/251‑4 (MQ=255)                                                                                                                                                                                                                                     aTCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTaac       >  1:167461/1‑248 (MQ=255)                                                                                                                                                                                                                                        gTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAgtg                                                                                                                                                                <  1:683125/94‑3 (MQ=255)                                                                                                                                                                                                                                          cAGCCACGAGATCCCGTAACGGACCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACCAGTGCATCGGATGACGTCAAATAAGTGTTACTTTTATTGGCAATACGATTAACAATCTGAACCAGTTCGTTTACTAATGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAACATCAAGATTGCTCAATACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAACCACTa  >  1:551862/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    ATCTAATGCAATCGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGGCGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACAAATTCTTTATCAGTTTTTTCCAGAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAATCTCATCAGCAACAATTTCAGCCATAACTAGAGTATCGGCTGAGGTCAAATAAGCACCGCTTTTATTGGCAACACGGTTAACAATCTGAACCAGTTCGTTTACCAGTGAGCTTCTTTCACTTTGAAGTTCATCAATCTTTTCAATATCAAGATTACTTAGTACTTCACTTCTAAGTAAGATTTTTTGTTGTTTGGTCAGCATGTTTAGCCACTA  >  1/856132‑856614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑