Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,177	A→G	intergenic (‑6520/‑66041)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,177	0	A	G	100.0%	217.5 / NA	79	intergenic (‑6520/‑66041)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/34);  total (45/34)

GTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCGCGAGTCATAGAACCAAAATTTACAAGCTCTTGCAATGTTTTTTTATTCTTACTGAATTTACGAATAGAAATGGATGTGCCATCTAATGCAATCGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGGCGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACAAATTCTTTATCAGTTTTTTCCAGAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAAT  >  1/855932‑856401                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 gTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAg                                                                                                                                                                                                                             >  1:235047/1‑251 (MQ=255)   aTGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTc                                                                                                                                                                                                                           <  1:249121/251‑1 (MQ=255)           tataATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTc                                                                                                                                                                                                                    >  1:2897/1‑250 (MQ=255)             taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGt                                                                                                                                                                                                                               <  1:640523/234‑1 (MQ=255)             taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCa                                                                                                                                                                                                                  >  1:422294/4‑250 (MQ=255)             taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCa                                                                                                                                                                                                                  >  1:697782/4‑250 (MQ=255)             taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCAc                                                                                                                                                                                                                 <  1:408684/248‑1 (MQ=255)             taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACAAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCa                                                                                                                                                                                                                  >  1:448071/4‑250 (MQ=255)                     tAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGAc                                                                                                                                                                                                         <  1:40588/251‑1 (MQ=255)                     tAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGAc                                                                                                                                                                                                         <  1:601913/251‑1 (MQ=255)                         gcattaaggagagttgttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTAc                                                                                                                                                                                                      >  1:635676/13‑250 (MQ=255)                          cattaaggagagttgttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACca                                                                                                                                                                                                    >  1:814888/12‑251 (MQ=255)                          cattaaggagagttgttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACca                                                                                                                                                                                                    >  1:819003/12‑251 (MQ=255)                          cattaaggagagttgttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACca                                                                                                                                                                                                    <  1:519038/240‑1 (MQ=255)                                      ttgttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATAc                                                                                                                                                                                          <  1:273647/249‑1 (MQ=255)                                      ttgttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACga                                                                                                                                                                                        <  1:323857/251‑2 (MQ=255)                                        gttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACgacg                                                                                                                                                                                      >  1:180538/1‑248 (MQ=255)                                        gttTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACgacg                                                                                                                                                                                      >  1:618678/1‑248 (MQ=255)                                                  aTCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACcatca                                                                                                                                                                                                 >  1:272976/1‑230 (MQ=255)                                                  aTCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGa                                                                                                                                                                            >  1:17210/1‑251 (MQ=255)                                                  aTCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGa                                                                                                                                                                            >  1:854231/1‑251 (MQ=255)                                                     cTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCa                                                                                                                                                                         >  1:550899/1‑251 (MQ=255)                                                     cTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCa                                                                                                                                                                         >  1:401498/1‑251 (MQ=255)                                                            accTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAAc                                                                                                                                                                   >  1:597092/1‑250 (MQ=255)                                                            accTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAAc                                                                                                                                                                   >  1:171629/1‑250 (MQ=255)                                                                 acacAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCttttt                                                                                                                                                             >  1:457632/1‑251 (MQ=255)                                                                   acaATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGc                                                                                                                                                           <  1:501982/251‑1 (MQ=255)                                                                    caATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCa                                                                                                                                                          >  1:211482/1‑251 (MQ=255)                                                                    caATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCa                                                                                                                                                          >  1:594926/1‑251 (MQ=255)                                                                      aTTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAat                                                                                                                                                        >  1:422609/1‑251 (MQ=255)                                                                         atatTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAAcc                                                                                                                                                                               >  1:132605/1‑225 (MQ=255)                                                                             ttactcgagATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGtt                                                                                                                                                 <  1:150133/243‑1 (MQ=255)                                                                                ctcgagATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAAt                                                                                                                                              >  1:593720/6‑251 (MQ=255)                                                                                ctcgagATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAAt                                                                                                                                              <  1:104960/246‑1 (MQ=255)                                                                                    agATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGt                                                                                                                                           <  1:538625/249‑1 (MQ=255)                                                                                      aTCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACga                                                                                                                                                                                        >  1:898626/1‑202 (MQ=255)                                                                                          ggCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGtt                                                                                                                                                 >  1:105483/1‑238 (MQ=255)                                                                                           gcagcgATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTAc                                                                                                                                    <  1:445950/250‑1 (MQ=255)                                                                                               cgataatTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAAcg                                                                                                                               <  1:374876/249‑2 (MQ=255)                                                                                                                                         aaTTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGtt                                                                                                                                                 <  1:603678/191‑1 (MQ=255)                                                                                                                                               cAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGAta                                                                               <  1:818981/251‑2 (MQ=255)                                                                                                                                                     ctTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGAta                                                                               >  1:557463/1‑244 (MQ=255)                                                                                                                                                     ctTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTa                                                                         >  1:136815/1‑251 (MQ=255)                                                                                                                                                         caagctttttttACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAggg                                                                      <  1:813685/245‑1 (MQ=255)                                                                                                                                                               ttttttACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTaa                                                               >  1:286497/1‑251 (MQ=255)                                                                                                                                                               ttttttACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTaa                                                               <  1:247948/251‑1 (MQ=255)                                                                                                                                                               ttttttACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTaa                                                               >  1:834553/1‑251 (MQ=255)                                                                                                                                                                tttttACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTg                                                                                                                                            >  1:53035/1‑173 (MQ=255)                                                                                                                                                                tttttACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTaa                                                               >  1:170150/1‑250 (MQ=255)                                                                                                                                                                      ctCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTaagat                                                        <  1:258190/250‑4 (MQ=255)                                                                                                                                                                        cTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACcat                                                                                                                                                                                                   <  1:893709/110‑1 (MQ=255)                                                                                                                                                                             tGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCAttt                                                 <  1:182602/251‑1 (MQ=255)                                                                                                                                                                              gaacttACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAAcc                                                                                     >  1:751771/5‑214 (MQ=255)                                                                                                                                                                              gaacttACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAAc                                                              <  1:649862/233‑1 (MQ=255)                                                                                                                                                                                acttACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGatat                                                      <  1:46413/241‑1 (MQ=255)                                                                                                                                                                                 cttACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAg                                             <  1:366335/250‑1 (MQ=255)                                                                                                                                                                                 cttACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAg                                             <  1:852340/250‑1 (MQ=255)                                                                                                                                                                                   tACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTa                                           >  1:834489/1‑251 (MQ=255)                                                                                                                                                                                           gAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAAccc                                                                                    <  1:460207/202‑1 (MQ=255)                                                                                                                                                                                               tGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCAtt                                                                          <  1:534121/208‑1 (MQ=255)                                                                                                                                                                                               tGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCacg                               >  1:863456/1‑249 (MQ=255)                                                                                                                                                                                                    gtaccATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTAc                                                                                                                                    <  1:133722/142‑1 (MQ=255)                                                                                                                                                                                                    gtaccATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGc                                                                     >  1:6628/4‑208 (MQ=255)                                                                                                                                                                                                    gtaccATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGAAGTCGGGAGTCCACTAGTGGACTACCatcatc                                                                                                                                                                                               >  1:566757/4‑86 (MQ=255)                                                                                                                                                                                                        cATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTg                                                                                                                                            <  1:322869/133‑1 (MQ=255)                                                                                                                                                                                                         aTCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCg                                        >  1:780385/1‑232 (MQ=255)                                                                                                                                                                                                         aTCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTa                     >  1:705393/1‑251 (MQ=255)                                                                                                                                                                                                         aTCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTa                     >  1:823350/1‑251 (MQ=255)                                                                                                                                                                                                               cgCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTaacggac               <  1:41424/250‑6 (MQ=255)                                                                                                                                                                                                                     cGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAc         <  1:698551/251‑1 (MQ=255)                                                                                                                                                                                                                       gTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTc                                                                                                                          >  1:137201/1‑136 (MQ=255)                                                                                                                                                                                                                       gTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGAta                                                                               >  1:53735/1‑178 (MQ=255)                                                                                                                                                                                                                       gTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAcc        <  1:698670/250‑1 (MQ=255)                                                                                                                                                                                                                            attacaacaTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGc                                                                     >  1:644040/4‑184 (MQ=255)                                                                                                                                                                                                                            attacaacaTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAACCCTCAAt  >  1:188624/4‑251 (MQ=255)                                                                                                                                                                                                                                   caTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGAta                                                                               <  1:415185/167‑2 (MQ=255)                                                                                                                                                                                                                                   caTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAAACCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATc                                                     <  1:37101/193‑1 (MQ=255)                                                                                                                                                                                                                                    aTTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAccc       >  1:329677/1‑238 (MQ=255)                                                                                                                                                                                                                                             gctaccATCGGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGACGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACGAATTCTTTATCAGTTTTTTCCAGAATACCGGCACGTTCAACCCAGATATCATTAGGGCCATTAACTAAGATATCATTTATAGTATCGTCAGCCACGAGATCCCGTAACGGACCATAAccc       >  1:594915/5‑229 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 GTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCGCGAGTCATAGAACCAAAATTTACAAGCTCTTGCAATGTTTTTTTATTCTTACTGAATTTACGAATAGAAATGGATGTGCCATCTAATGCAATCGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGACTACCATCATCAATACGGCGACCAACCCGAGCAACTAACCTTTTTGCAATATCAGTTAATTGTTCATTACTAACAAATTCTTTATCAGTTTTTTCCAGAATACCAGCACGTTCAACCCAGATGTCATTAGGGCCATTAACTAAAATATCATTTATAGTATCGTCAGCCATGAGATCTCGTAATGGTCCATAACCCTCAAT  >  1/855932‑856401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑