Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,964	T→G	intergenic (‑6307/‑66254)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,964	0	T	G	100.0%	209.3 / NA	73	intergenic (‑6307/‑66254)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/34);  total (39/34)

CTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCGCGAGTCATAGAACCAAAATTTACAAGCTCTTGCAATGTTTTTTTATTCTTACTGAATTTACGAATAGAAATGGATGTGCCATCTAATGCAATCGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGAC  >  1/855729‑856202                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTAccg                                                                                                                                                                                                                                 >  1:205371/1‑250 (MQ=255)    gTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGAt                                                                                                                                                                                                                              >  1:245260/1‑251 (MQ=255)          tctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTa                                                                                                                                                                                                                        >  1:494249/3‑251 (MQ=255)           ctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                                                                                       <  1:334492/250‑1 (MQ=255)               aaTGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTaccac                                                                                                                                                                                                                    <  1:829725/250‑1 (MQ=255)                           ccacGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATtat                                                                                                                                                                                                       >  1:144208/1‑251 (MQ=255)                             acGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTatat                                                                                                                                                                                                     <  1:644994/251‑1 (MQ=255)                               ggCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                                                                                                                                                                                   >  1:493036/1‑251 (MQ=255)                                gCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                                                                                                                                                                                   <  1:889650/250‑1 (MQ=255)                                      acctacgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                                                                                       <  1:361783/217‑1 (MQ=255)                                             ataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTGCCACCTGACACAATTATATTTACTCGAGATCTGgc                                                                                                                                                                                     <  1:63340/251‑1 (MQ=255)                                                         ggACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaa                                                                                                                                                                         >  1:480766/1‑251 (MQ=255)                                                            cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                                                                                       >  1:146158/1‑202 (MQ=255)                                                            cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGgc                                                                                                                                                                                     >  1:818038/1‑236 (MQ=255)                                                            cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaaaaa                                                                                                                                                                      >  1:608606/1‑248 (MQ=255)                                                            cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaaaaa                                                                                                                                                                      >  1:389156/1‑248 (MQ=255)                                                              cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                                                                                       <  1:794791/200‑1 (MQ=255)                                                              cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGgc                                                                                                                                                                                     <  1:311426/234‑1 (MQ=255)                                                              cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAAt                                                                                                                                                                    >  1:241731/1‑251 (MQ=255)                                                                tacgtagtGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAAttt                                                                                                                                                                  >  1:96476/8‑250 (MQ=255)                                                                          aTTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATcg                                                                                                                                                         >  1:554324/1‑250 (MQ=255)                                                                                           gCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                                                                                       >  1:558827/1‑171 (MQ=255)                                                                                           gCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCgaaa                                                                                                                                       >  1:354902/1‑251 (MQ=255)                                                                                            cATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAAt                                                                                                                                      >  1:815454/1‑251 (MQ=255)                                                                                                   acctCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAg                                                                                                                               <  1:579715/248‑1 (MQ=255)                                                                                                                        ccGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTac                                                                                                           <  1:46410/250‑2 (MQ=255)                                                                                                                          ggCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTactc                                                                                                         <  1:628033/250‑4 (MQ=255)                                                                                                                                   tgTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGaac                                                                                                <  1:384333/249‑2 (MQ=255)                                                                                                                                    gTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                                                                                                                                                                                   >  1:100438/1‑150 (MQ=255)                                                                                                                                     tttCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCtttttt                                                                                                              >  1:626718/1‑234 (MQ=255)                                                                                                                                            gCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTAct                                                                                                                                                                                                <  1:722098/145‑2 (MQ=255)                                                                                                                                            gCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAAtgg                                                                                      >  1:508393/1‑249 (MQ=255)                                                                                                                                              gTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTActcg                                                                                                                                                                                              >  1:409918/1‑142 (MQ=255)                                                                                                                                                 caacaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGa                                                                                                                                              >  1:656595/1‑190 (MQ=255)                                                                                                                                                   acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAAc                                                                                                                                            <  1:560235/190‑1 (MQ=255)                                                                                                                                                   acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga                                                                               >  1:450213/1‑251 (MQ=255)                                                                                                                                                   acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga                                                                               >  1:207061/1‑251 (MQ=255)                                                                                                                                                   acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga                                                                               >  1:843206/1‑251 (MQ=255)                                                                                                                                                   acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga                                                                               >  1:290863/1‑251 (MQ=255)                                                                                                                                                          gttGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGAtgtac                                                                          <  1:277005/247‑3 (MQ=255)                                                                                                                                                           ttGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCAt                                                                       <  1:144450/250‑1 (MQ=255)                                                                                                                                                             gTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCa                                                                        <  1:652590/248‑1 (MQ=255)                                                                                                                                                             gTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCAt                                                                       >  1:321550/1‑249 (MQ=255)                                                                                                                                                              ttCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCt                                                                     <  1:883240/250‑1 (MQ=255)                                                                                                                                                               tCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTTAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCa                                                                        <  1:333926/246‑1 (MQ=255)                                                                                                                                                                  aaacgcaattcagCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTaacgc                                                                <  1:437599/239‑4 (MQ=255)                                                                                                                                                                                 gTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAAtta                                                  >  1:521795/1‑248 (MQ=255)                                                                                                                                                                                 gTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAAtta                                                  >  1:401642/1‑248 (MQ=255)                                                                                                                                                                                   aTCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATtacaa                                               >  1:544612/1‑251 (MQ=255)                                                                                                                                                                                     cttctAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTaacgc                                                                <  1:595869/232‑4 (MQ=255)                                                                                                                                                                                     cttctAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTacaaca                                             >  1:489297/1‑251 (MQ=255)                                                                                                                                                                                            tgtgATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTaaa                                       >  1:516466/1‑250 (MQ=255)                                                                                                                                                                                             gtgATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACg                                     >  1:418083/1‑251 (MQ=255)                                                                                                                                                                                                       gTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCttttttt                                                                                                             <  1:161846/169‑1 (MQ=255)                                                                                                                                                                                                       gTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGAt                                                                              <  1:49684/200‑1 (MQ=255)                                                                                                                                                                                                       gTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATcg                           >  1:268674/1‑250 (MQ=255)                                                                                                                                                                                                         tCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATcggg                         <  1:761072/251‑4 (MQ=255)                                                                                                                                                                                                            gTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGa                                                                                                                                              <  1:765229/131‑1 (MQ=255)                                                                                                                                                                                                            gTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAg                      >  1:235047/1‑251 (MQ=255)                                                                                                                                                                                                              aTGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTc                    <  1:249121/251‑1 (MQ=255)                                                                                                                                                                                                                   aaaTATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGAt                                                                              <  1:377932/188‑1 (MQ=255)                                                                                                                                                                                                                      tataATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTc             >  1:2897/1‑250 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATtacaa                                               >  1:571432/4‑214 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGt                        <  1:640523/234‑1 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCa           >  1:697782/4‑250 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCa           >  1:422294/4‑250 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCAc          <  1:408684/248‑1 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACAAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCa           >  1:448071/4‑250 (MQ=255)                                                                                                                                                                                                                        taattAGATAGCGCATTAAGGAGAGCTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTaac                                                                  <  1:429363/192‑2 (MQ=255)                                                                                                                                                                                                                             aGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAAttac                                                 <  1:731078/207‑4 (MQ=255)                                                                                                                                                                                                                             aGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATc                            <  1:50244/228‑1 (MQ=255)                                                                                                                                                                                                                                tAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGAc  <  1:601913/251‑1 (MQ=255)                                                                                                                                                                                                                                tAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCTAACGCAATCGGTGCAATTACAACATTTAAACGGCTACCATCGGGTAGTCGGGAGTCCACTAGTGGAc  <  1:40588/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               CTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCGCGAGTCATAGAACCAAAATTTACAAGCTCTTGCAATGTTTTTTTATTCTTACTGAATTTACGAATAGAAATGGATGTGCCATCTAATGCAATCGGTGCAATAACAACATTTAAACGGCTGCCATCAGGTAGTCGGGAGTCCACTAGTGGAC  >  1/855729‑856202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑