Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,901	G→A	intergenic (‑6244/‑66317)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,901	0	G	A	100.0%	204.1 / NA	76	intergenic (‑6244/‑66317)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/37);  total (39/37)

TGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCGCGAGTCATAGAACCAAAATTTACAAGCTCTTGCAATGTTTTTTTATTCTTACTGAATTTACGAATAGAAATGGATGTGCCATCT  >  1/855667‑856135                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                                                                                                                                                            <  1:846900/251‑4 (MQ=255) tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTATATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaa                                                                                                                                                                                                                              >  1:426233/1‑248 (MQ=255)     tcccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgat                                                                                                                                                                                                                        >  1:847545/4‑251 (MQ=255)      cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                                                                                                                                                            <  1:664584/244‑4 (MQ=255)      cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg                                                                                                                                                                                                                       >  1:298321/3‑251 (MQ=255)          aGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGaca                                                                                                                                                                                                                    <  1:803416/250‑1 (MQ=255)              gtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGtt                                                                                                                                                                                                                >  1:363174/2‑250 (MQ=255)                         cAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                                                                                                     <  1:89481/250‑1 (MQ=255)                         cAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                                                                                                     <  1:483751/250‑1 (MQ=255)                            cGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAatat                                                                                                                                                                                                 <  1:5915/251‑1 (MQ=255)                                 aCATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTAtct                                                                                                                                                                                                                                   <  1:354185/212‑1 (MQ=255)                                 aCATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTa                                                                                                                                                                                            <  1:473526/251‑1 (MQ=255)                                    tAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                                                                                                         <  1:188748/251‑1 (MQ=255)                                    tAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                                                                                                         <  1:216418/251‑1 (MQ=255)                                               tGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                                                                                                     >  1:109779/1‑228 (MQ=255)                                               tGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaa                                                                                                                                                                               <  1:519060/250‑1 (MQ=255)                                                gaccagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                                                                                                         >  1:471849/9‑239 (MQ=255)                                                 accagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaagg                                                                                                                                                                             <  1:102206/243‑2 (MQ=255)                                                  ccagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaaggag                                                                                                                                                                           <  1:103052/245‑4 (MQ=255)                                                     gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCAt                                                                                                                                                                                  <  1:475082/238‑1 (MQ=255)                                                     gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaaggagagt                                                                                                                                                                        >  1:563851/4‑245 (MQ=255)                                                               cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg                                                                                                                                                                                                                       >  1:427258/1‑194 (MQ=255)                                                               cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTAccg                                                                                                                                                              >  1:205371/1‑250 (MQ=255)                                                                  gTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGAt                                                                                                                                                           >  1:245260/1‑251 (MQ=255)                                                                        tctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTa                                                                                                                                                     >  1:494249/3‑251 (MQ=255)                                                                         ctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                    <  1:334492/250‑1 (MQ=255)                                                                             aaTGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTaccac                                                                                                                                                 <  1:829725/250‑1 (MQ=255)                                                                                         ccacGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATtat                                                                                                                                    >  1:144208/1‑251 (MQ=255)                                                                                           acGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTatat                                                                                                                                  <  1:644994/251‑1 (MQ=255)                                                                                             ggCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                                                                                                                >  1:493036/1‑251 (MQ=255)                                                                                              gCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                                                                                                                <  1:889650/250‑1 (MQ=255)                                                                                                   cacctacgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                                                                                                         <  1:217916/180‑1 (MQ=255)                                                                                                    acctacgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                    <  1:361783/217‑1 (MQ=255)                                                                                                        acgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                                                                                                     <  1:300534/168‑1 (MQ=255)                                                                                                           ataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAg                                                                                                                                                                                       >  1:422013/1‑182 (MQ=255)                                                                                                           ataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTGCCACCTGACACAATTATATTTACTCGAGATCTGgc                                                                                                                  <  1:63340/251‑1 (MQ=255)                                                                                                               taCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGac                                                                                                                                                                                                                     >  1:763185/1‑148 (MQ=255)                                                                                                                  gCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaagg                                                                                                                                                                             >  1:679120/1‑184 (MQ=255)                                                                                                                       ggACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaa                                                                                                      >  1:480766/1‑251 (MQ=255)                                                                                                                          cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                    >  1:146158/1‑202 (MQ=255)                                                                                                                          cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGgc                                                                                                                  >  1:818038/1‑236 (MQ=255)                                                                                                                          cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaaaaa                                                                                                   >  1:389156/1‑248 (MQ=255)                                                                                                                          cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaaaaa                                                                                                   >  1:608606/1‑248 (MQ=255)                                                                                                                            cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                    <  1:794791/200‑1 (MQ=255)                                                                                                                            cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGgc                                                                                                                  <  1:311426/234‑1 (MQ=255)                                                                                                                            cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAAt                                                                                                 >  1:241731/1‑251 (MQ=255)                                                                                                                              tacgtagtGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAAttt                                                                                               >  1:96476/8‑250 (MQ=255)                                                                                                                                        aTTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATcg                                                                                      >  1:554324/1‑250 (MQ=255)                                                                                                                                                    atcctGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                                                                                                                                                            <  1:835886/100‑4 (MQ=255)                                                                                                                                                    atcctGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTa                                                                                                                                                                                            >  1:353327/5‑136 (MQ=255)                                                                                                                                                         gCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                                                                                                    >  1:558827/1‑171 (MQ=255)                                                                                                                                                         gCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCgaaa                                                                    >  1:354902/1‑251 (MQ=255)                                                                                                                                                          cATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                                                                                                     >  1:451535/1‑121 (MQ=255)                                                                                                                                                          cATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAAt                                                                   >  1:815454/1‑251 (MQ=255)                                                                                                                                                            tAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                                                                                                         <  1:16537/131‑1 (MQ=255)                                                                                                                                                                 acctCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAg                                                            <  1:579715/248‑1 (MQ=255)                                                                                                                                                                                      ccGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTac                                        <  1:46410/250‑2 (MQ=255)                                                                                                                                                                                        ggCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTactc                                      <  1:628033/250‑4 (MQ=255)                                                                                                                                                                                                 tgTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGaac                             <  1:384333/249‑2 (MQ=255)                                                                                                                                                                                                  gTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                                                                                                                >  1:100438/1‑150 (MQ=255)                                                                                                                                                                                                   tttCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCtttttt                                           >  1:626718/1‑234 (MQ=255)                                                                                                                                                                                                          gCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTAct                                                                                                                             <  1:722098/145‑2 (MQ=255)                                                                                                                                                                                                          gCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAAtgg                   >  1:508393/1‑249 (MQ=255)                                                                                                                                                                                                            gTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTActcg                                                                                                                           >  1:409918/1‑142 (MQ=255)                                                                                                                                                                                                               caacaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGa                                                                           >  1:656595/1‑190 (MQ=255)                                                                                                                                                                                                                 acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAAc                                                                         <  1:560235/190‑1 (MQ=255)                                                                                                                                                                                                                 acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga            >  1:450213/1‑251 (MQ=255)                                                                                                                                                                                                                 acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga            >  1:843206/1‑251 (MQ=255)                                                                                                                                                                                                                 acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga            >  1:290863/1‑251 (MQ=255)                                                                                                                                                                                                                 acaTGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGaatggaaatgga            >  1:207061/1‑251 (MQ=255)                                                                                                                                                                                                                        gttGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGAtgtac       <  1:277005/247‑3 (MQ=255)                                                                                                                                                                                                                         ttGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCAt    <  1:144450/250‑1 (MQ=255)                                                                                                                                                                                                                           gTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCa     <  1:652590/248‑1 (MQ=255)                                                                                                                                                                                                                           gTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCAt    >  1:321550/1‑249 (MQ=255)                                                                                                                                                                                                                            ttCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCATCt  <  1:883240/250‑1 (MQ=255)                                                                                                                                                                                                                             tCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTTAAAAATTTGCCATATCGCGGGTCATAGAACCGAAATTTACAAGCTCTTGCAAGCTTTTTTTACTCTTACTGAACTTACGAATGGAAATGGATGTACCa     <  1:333926/246‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           TGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCGCGAGTCATAGAACCAAAATTTACAAGCTCTTGCAATGTTTTTTTATTCTTACTGAATTTACGAATAGAAATGGATGTGCCATCT  >  1/855667‑856135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑