Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,817	T→C	intergenic (‑6160/‑66401)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,817	0	T	C	100.0%	229.1 / NA	79	intergenic (‑6160/‑66401)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/34);  total (45/34)

TATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACTTTCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCG  >  1/855577‑856051                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa                                                                                                                                                                                                                                  >  1:689306/20‑251 (MQ=255) gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa                                                                                                                                                                                                                                  >  1:215324/20‑251 (MQ=255)      ttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGCGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCg                                                                                                                                                                                                                             >  1:28129/15‑251 (MQ=255)         ctgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGc                                                                                                                                                                                                                            >  1:538650/12‑249 (MQ=255)                tgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATg                                                                                                                                                                                                                     >  1:292012/5‑249 (MQ=255)                  caatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCa                                                                                                                                                                                                                 >  1:117009/3‑251 (MQ=255)                                     ttccaaCGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGttt                                                                                                                                                                                              <  1:41889/247‑1 (MQ=255)                                      tccaaCGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGcc                                                                                                                                                                                                                           >  1:15671/4‑221 (MQ=255)                                                   acgCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTacaac                                                                                                                                                                                 <  1:407762/248‑1 (MQ=255)                                                         tCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                                                          <  1:782473/251‑2 (MQ=255)                                                          cGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                                                          >  1:475616/1‑249 (MQ=255)                                                            aCATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGc                                                                                                                                                                                                             <  1:643934/213‑1 (MQ=255)                                                                  tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCGTCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGt                                                                                                                                                                                                                                    >  1:754580/1‑184 (MQ=255)                                                                  tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                                                                                                                                                 >  1:383791/1‑251 (MQ=255)                                                                  tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                                                                                                                                                 >  1:323908/1‑251 (MQ=255)                                                                  tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                                                                                                                                                 >  1:452672/1‑251 (MQ=255)                                                                    aCCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACAt                                                                                                                                                                               >  1:655968/1‑235 (MQ=255)                                                                          ctctCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaatt                                                                                                                                                          >  1:526348/4‑247 (MQ=255)                                                                             tCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagc                                                                                                                                                      <  1:496729/251‑8 (MQ=255)                                                                              ccAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagca                                                                                                                                                     >  1:149090/1‑243 (MQ=255)                                                                               caatcGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                                                                    <  1:829310/247‑10 (MQ=255)                                                                                    gagaTGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                                                          >  1:28837/1‑223 (MQ=255)                                                                                         gTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCAt                                                                                                                                                                                                                                       >  1:237478/1‑158 (MQ=255)                                                                                           tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                                                                        <  1:846900/251‑4 (MQ=255)                                                                                           tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTATATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaa                                                                                                                                          >  1:426233/1‑248 (MQ=255)                                                                                               tcccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgat                                                                                                                                    >  1:847545/4‑251 (MQ=255)                                                                                                cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                                                                        <  1:664584/244‑4 (MQ=255)                                                                                                cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg                                                                                                                                   >  1:298321/3‑251 (MQ=255)                                                                                                    aGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGaca                                                                                                                                <  1:803416/250‑1 (MQ=255)                                                                                                      gggtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                                                                    >  1:113111/4‑219 (MQ=255)                                                                                                       ggtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAgg                                                                                                                                                                                         <  1:126127/188‑1 (MQ=255)                                                                                                        gtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGtt                                                                                                                            >  1:363174/2‑250 (MQ=255)                                                                                                                   cAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                 <  1:483751/250‑1 (MQ=255)                                                                                                                   cAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                 <  1:89481/250‑1 (MQ=255)                                                                                                                      cGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAatat                                                                                                             <  1:5915/251‑1 (MQ=255)                                                                                                                           aCATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTAtct                                                                                                                                               <  1:354185/212‑1 (MQ=255)                                                                                                                           aCATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTa                                                                                                        <  1:473526/251‑1 (MQ=255)                                                                                                                              tAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                     <  1:216418/251‑1 (MQ=255)                                                                                                                              tAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                     <  1:188748/251‑1 (MQ=255)                                                                                                                                gAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                                                          >  1:655922/1‑179 (MQ=255)                                                                                                                                         tGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                 >  1:109779/1‑228 (MQ=255)                                                                                                                                         tGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaa                                                                                           <  1:519060/250‑1 (MQ=255)                                                                                                                                          gaccagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                     >  1:471849/9‑239 (MQ=255)                                                                                                                                           accagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaagg                                                                                         <  1:102206/243‑2 (MQ=255)                                                                                                                                            ccagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaaggag                                                                                       <  1:103052/245‑4 (MQ=255)                                                                                                                                              agtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                                                                    >  1:718168/5‑179 (MQ=255)                                                                                                                                               gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                                                                    <  1:258161/184‑10 (MQ=255)                                                                                                                                               gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCAt                                                                                              <  1:475082/238‑1 (MQ=255)                                                                                                                                               gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaaggagagt                                                                                    >  1:563851/4‑245 (MQ=255)                                                                                                                                                 gttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAgg                                                                                                                                                                           >  1:373503/2‑162 (MQ=255)                                                                                                                                                         cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTc                                                                                                                                                                                             <  1:163982/136‑1 (MQ=255)                                                                                                                                                         cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg                                                                                                                                   >  1:427258/1‑194 (MQ=255)                                                                                                                                                         cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTAccg                                                                          >  1:205371/1‑250 (MQ=255)                                                                                                                                                            gTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGAt                                                                       >  1:245260/1‑251 (MQ=255)                                                                                                                                                                  tctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTa                                                                 >  1:494249/3‑251 (MQ=255)                                                                                                                                                                   ctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                <  1:334492/250‑1 (MQ=255)                                                                                                                                                                       aaTGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTaccac                                                             <  1:829725/250‑1 (MQ=255)                                                                                                                                                                                   ccacGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATtat                                                >  1:144208/1‑251 (MQ=255)                                                                                                                                                                                     acGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTatat                                              <  1:644994/251‑1 (MQ=255)                                                                                                                                                                                       ggCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                            >  1:493036/1‑251 (MQ=255)                                                                                                                                                                                        gCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt                                            <  1:889650/250‑1 (MQ=255)                                                                                                                                                                                             cacctacgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                                                                     <  1:217916/180‑1 (MQ=255)                                                                                                                                                                                              acctacgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                <  1:361783/217‑1 (MQ=255)                                                                                                                                                                                                  acgataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                                                                 <  1:300534/168‑1 (MQ=255)                                                                                                                                                                                                     ataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAg                                                                                                   >  1:422013/1‑182 (MQ=255)                                                                                                                                                                                                     ataataCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTGCCACCTGACACAATTATATTTACTCGAGATCTGgc                              <  1:63340/251‑1 (MQ=255)                                                                                                                                                                                                         taCGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGac                                                                                                                                 >  1:763185/1‑148 (MQ=255)                                                                                                                                                                                                            gCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAgg                                                                                                                                                                           <  1:649693/103‑1 (MQ=255)                                                                                                                                                                                                            gCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaagg                                                                                         >  1:679120/1‑184 (MQ=255)                                                                                                                                                                                                                 ggACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaa                  >  1:480766/1‑251 (MQ=255)                                                                                                                                                                                                                    cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                >  1:146158/1‑202 (MQ=255)                                                                                                                                                                                                                    cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGgc                              >  1:818038/1‑236 (MQ=255)                                                                                                                                                                                                                    cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaaaaa               >  1:608606/1‑248 (MQ=255)                                                                                                                                                                                                                    cGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTaaaaa               >  1:389156/1‑248 (MQ=255)                                                                                                                                                                                                                      cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                                                                <  1:794791/200‑1 (MQ=255)                                                                                                                                                                                                                      cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGgc                              <  1:311426/234‑1 (MQ=255)                                                                                                                                                                                                                      cATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAAt             >  1:241731/1‑251 (MQ=255)                                                                                                                                                                                                                        tacgtagtGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAAttt           >  1:96476/8‑250 (MQ=255)                                                                                                                                                                                                                                  aTTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATATTTACTCGAGATCTGGCAGCGATAATTAAAAAATTTGCCATATcg  >  1:554324/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           TATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACTTTCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTTACCCGGGATCTGGCAGCAATAATTAAGAAATTCGCCATTTCG  >  1/855577‑856051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑