Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,772	A→G	intergenic (‑6115/‑66446)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,772	0	A	G	100.0%	200.9 / NA	73	intergenic (‑6115/‑66446)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/31);  total (42/31)

TAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACTTTCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTT  >  1/855534‑856009                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              tgaTTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCa                                                                                                                                                                                                                                   >  1:782966/3‑251 (MQ=255)  gaTTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAg                                                                                                                                                                                                                                  >  1:281034/2‑251 (MQ=255)                                            gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgtag                                                                                                                                                                                                                     >  1:275003/20‑219 (MQ=255)                                            gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa                                                                                                                                                                                        >  1:689306/20‑251 (MQ=255)                                            gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa                                                                                                                                                                                        >  1:215324/20‑251 (MQ=255)                                               atttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt                                                                                                                                                                                                                       >  1:743762/17‑216 (MQ=255)                                                 ttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGCGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCg                                                                                                                                                                                   >  1:28129/15‑251 (MQ=255)                                                    ctgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGc                                                                                                                                                                                  >  1:538650/12‑249 (MQ=255)                                                         gatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt                                                                                                                                                                                                                       >  1:659428/7‑206 (MQ=255)                                                           tgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATg                                                                                                                                                                           >  1:292012/5‑249 (MQ=255)                                                             caatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCa                                                                                                                                                                       >  1:117009/3‑251 (MQ=255)                                                               atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgtagtgc                                                                                                                                                                                                                  >  1:872275/1‑200 (MQ=255)                                                               atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt                                                                                                                                                                                                                       <  1:776148/201‑2 (MQ=255)                                                                                ttccaaCGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGttt                                                                                                                                                    <  1:41889/247‑1 (MQ=255)                                                                                 tccaaCGGTAGGGACGCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGcc                                                                                                                                                                                 >  1:15671/4‑221 (MQ=255)                                                                                             gacgCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGAc                                                                                                                                                                                                                               <  1:175010/160‑1 (MQ=255)                                                                                              acgCATTCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTacaac                                                                                                                                       <  1:407762/248‑1 (MQ=255)                                                                                                    tCGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                <  1:782473/251‑2 (MQ=255)                                                                                                     cGACATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                >  1:475616/1‑249 (MQ=255)                                                                                                       aCATCATAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGc                                                                                                                                                                   <  1:643934/213‑1 (MQ=255)                                                                                                             tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCGTCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGt                                                                                                                                                                                          >  1:754580/1‑184 (MQ=255)                                                                                                             tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATAc                                                                                                                                                                                                                         >  1:257180/1‑153 (MQ=255)                                                                                                             tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                                                                                                       >  1:452672/1‑251 (MQ=255)                                                                                                             tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                                                                                                       >  1:383791/1‑251 (MQ=255)                                                                                                             tAACCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                                                                                                       >  1:323908/1‑251 (MQ=255)                                                                                                               aCCATACTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACAt                                                                                                                                     >  1:655968/1‑235 (MQ=255)                                                                                                                     ctctCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaatt                                                                                                                >  1:526348/4‑247 (MQ=255)                                                                                                                       ctCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGAc                                                                                                                                                                                                                               >  1:628576/2‑137 (MQ=255)                                                                                                                        tCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagc                                                                                                            <  1:496729/251‑8 (MQ=255)                                                                                                                         ccAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagca                                                                                                           >  1:149090/1‑243 (MQ=255)                                                                                                                          caatcGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                          <  1:829310/247‑10 (MQ=255)                                                                                                                               gagaTGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                >  1:28837/1‑223 (MQ=255)                                                                                                                                  aTGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATAc                                                                                                                                                                                                                                        <  1:305969/117‑1 (MQ=255)                                                                                                                                    gTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCAt                                                                                                                                                                                             >  1:237478/1‑158 (MQ=255)                                                                                                                                      tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                              <  1:846900/251‑4 (MQ=255)                                                                                                                                      tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTATATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaa                                                                                                >  1:426233/1‑248 (MQ=255)                                                                                                                                          tcccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgat                                                                                          >  1:847545/4‑251 (MQ=255)                                                                                                                                           cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg                                                                                              <  1:664584/244‑4 (MQ=255)                                                                                                                                           cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg                                                                                         >  1:298321/3‑251 (MQ=255)                                                                                                                                               aGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGaca                                                                                      <  1:803416/250‑1 (MQ=255)                                                                                                                                                 gggtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                          >  1:113111/4‑219 (MQ=255)                                                                                                                                                  ggtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAgg                                                                                                                                               <  1:126127/188‑1 (MQ=255)                                                                                                                                                   gtATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGtt                                                                                  >  1:363174/2‑250 (MQ=255)                                                                                                                                                              cAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                       <  1:89481/250‑1 (MQ=255)                                                                                                                                                              cAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                       <  1:483751/250‑1 (MQ=255)                                                                                                                                                                 cGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAatat                                                                   <  1:5915/251‑1 (MQ=255)                                                                                                                                                                      aCATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTAtct                                                                                                     <  1:354185/212‑1 (MQ=255)                                                                                                                                                                      aCATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTa                                                              <  1:473526/251‑1 (MQ=255)                                                                                                                                                                         tAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                           <  1:188748/251‑1 (MQ=255)                                                                                                                                                                         tAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                           <  1:216418/251‑1 (MQ=255)                                                                                                                                                                           gAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                                                                                                                >  1:655922/1‑179 (MQ=255)                                                                                                                                                                                    tGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGaa                                                                       >  1:109779/1‑228 (MQ=255)                                                                                                                                                                                    tGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaa                                                 <  1:519060/250‑1 (MQ=255)                                                                                                                                                                                     gaccagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGAt                                                           >  1:471849/9‑239 (MQ=255)                                                                                                                                                                                      accagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaagg                                               <  1:102206/243‑2 (MQ=255)                                                                                                                                                                                       ccagtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaaggag                                             <  1:103052/245‑4 (MQ=255)                                                                                                                                                                                         agtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                          >  1:718168/5‑179 (MQ=255)                                                                                                                                                                                          gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTAc                                                                                                                                                                                                         <  1:834160/89‑1 (MQ=255)                                                                                                                                                                                          gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                                                                                                          <  1:258161/184‑10 (MQ=255)                                                                                                                                                                                          gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCAt                                                    <  1:475082/238‑1 (MQ=255)                                                                                                                                                                                          gtgttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATtaaggagagt                                          >  1:563851/4‑245 (MQ=255)                                                                                                                                                                                            gttCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAgg                                                                                                                                 >  1:373503/2‑162 (MQ=255)                                                                                                                                                                                                    cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTc                                                                                                                                                   <  1:163982/136‑1 (MQ=255)                                                                                                                                                                                                    cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg                                                                                         >  1:427258/1‑194 (MQ=255)                                                                                                                                                                                                    cTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTAccg                                >  1:205371/1‑250 (MQ=255)                                                                                                                                                                                                       gTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGAt                             >  1:245260/1‑251 (MQ=255)                                                                                                                                                                                                             tctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTa                       >  1:494249/3‑251 (MQ=255)                                                                                                                                                                                                              ctGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTac                      <  1:334492/250‑1 (MQ=255)                                                                                                                                                                                                                  aaTGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTaccac                   <  1:829725/250‑1 (MQ=255)                                                                                                                                                                                                                              ccacGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATtat      >  1:144208/1‑251 (MQ=255)                                                                                                                                                                                                                                acGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTatat    <  1:644994/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt  >  1:493036/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGACACGTTCGGTATGGGAAATATAATTAGATAGCGCATTAAGGAGAGTTGTTTTACCGGATCCTGTACCACCTGACACAATTATAttt  <  1:889650/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              TAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACTTTCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACACGTTCGGTATGGGAAATATAGTTAGATAGCGCATTAAGTAAGGTTGTTTTACCAGATCCTGTACCACCTGACACAATTATATTT  >  1/855534‑856009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑