Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,680	C→G	intergenic (‑6023/‑66538)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,680	0	C	G	100.0%	182.0 / NA	66	intergenic (‑6023/‑66538)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (36/30);  total (36/30)

TTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACT‑T‑TCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACA  >  1/855458‑855925                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ttACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc                                                                                                                                                                                                                           <  1:323313/251‑1 (MQ=255) ttACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTCTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc                                                                                                                                                                                                                           <  1:438528/251‑1 (MQ=255)     ttGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCg                                                                                                                                                                                                                       <  1:105586/251‑1 (MQ=255)     ttGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCg                                                                                                                                                                                                                       <  1:833597/251‑1 (MQ=255)                gAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGAccagtgtt                                                                                                                                                                                                            >  1:401941/1‑245 (MQ=255)                                     aTTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa                                                                                                                                                                                       <  1:775030/251‑1 (MQ=255)                                     aTTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa                                                                                                                                                                                       <  1:145787/251‑1 (MQ=255)                                           ccattttCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTtc                                                                                                                                                                                 <  1:390491/248‑1 (MQ=255)                                               tttCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTcca                                                                                                                                                                             >  1:462289/1‑251 (MQ=255)                                                ttccatacccattatttCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTcca                                                                                                                                                                             >  1:676093/17‑250 (MQ=255)                                                 tccatacccattatttCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCAtc                                                                                                                                                                                                        <  1:737220/207‑2 (MQ=255)                                                                   gtaatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa                                                                                                                                                                                       <  1:846452/218‑1 (MQ=255)                                                                   gtaatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat                                                                                                                                                         >  1:563338/4‑242 (MQ=255)                                                                    taatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgata                                                                                                                                                        <  1:405504/249‑11 (MQ=255)                                                                    taatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat                                                                                                                                                         <  1:418153/248‑10 (MQ=255)                                                                    taatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat                                                                                                                                                         <  1:629222/248‑10 (MQ=255)                                                                             tgaTTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCa                                                                                                                                               >  1:782966/3‑251 (MQ=255)                                                                              gaTTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAg                                                                                                                                              >  1:281034/2‑251 (MQ=255)                                                                                                                        gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgtag                                                                                                                                 >  1:275003/20‑219 (MQ=255)                                                                                                                        gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa                                                                                                    >  1:215324/20‑251 (MQ=255)                                                                                                                        gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa                                                                                                    >  1:689306/20‑251 (MQ=255)                                                                                                                           atttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa                                                                                                                                                                                       >  1:316392/17‑165 (MQ=255)                                                                                                                           atttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt                                                                                                                                   >  1:743762/17‑216 (MQ=255)                                                                                                                             ttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGCGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCg                                                                                               >  1:28129/15‑251 (MQ=255)                                                                                                                                ctgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGc                                                                                              >  1:538650/12‑249 (MQ=255)                                                                                                                                     gatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt                                                                                                                                   >  1:659428/7‑206 (MQ=255)                                                                                                                                      atgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCAtcg                                                                                                                                                                                                       >  1:30257/6‑136 (MQ=255)                                                                                                                                       tgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATg                                                                                       >  1:292012/5‑249 (MQ=255)                                                                                                                                         caatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat                                                                                                                                                         <  1:807965/181‑10 (MQ=255)                                                                                                                                         caatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCa                                                                                   >  1:117009/3‑251 (MQ=255)                                                                                                                                           atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCAtcgc                                                                                                                                                                                                      <  1:12367/134‑4 (MQ=255)                                                                                                                                           atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgtagtgc                                                                                                                              >  1:872275/1‑200 (MQ=255)                                                                                                                                           atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt                                                                                                                                   <  1:776148/201‑2 (MQ=255)                                                                                                                                                            ttccaaCGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGttt                                                                <  1:41889/247‑1 (MQ=255)                                                                                                                                                             tccaaCGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGcc                                                                                             >  1:15671/4‑221 (MQ=255)                                                                                                                                                                   ggTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacga                                                                                                                                                          <  1:706188/154‑9 (MQ=255)                                                                                                                                                                    gTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc                                                                                                                                                                                                                           <  1:218801/88‑1 (MQ=255)                                                                                                                                                                         gacgCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGAc                                                                                                                                           <  1:175010/160‑1 (MQ=255)                                                                                                                                                                          acgCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTacaac                                                   <  1:407762/248‑1 (MQ=255)                                                                                                                                                                                tCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                            <  1:782473/251‑2 (MQ=255)                                                                                                                                                                                 cGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                            >  1:475616/1‑249 (MQ=255)                                                                                                                                                                                   aCATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGc                                                                               <  1:643934/213‑1 (MQ=255)                                                                                                                                                                                      tcatAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTccac                                                                                                                                                                            <  1:330552/117‑1 (MQ=255)                                                                                                                                                                                       catAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGCTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacga                                                                                                                                                          <  1:346730/134‑9 (MQ=255)                                                                                                                                                                                         tAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCGTCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGt                                                                                                      >  1:754580/1‑184 (MQ=255)                                                                                                                                                                                         tAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATAc                                                                                                                                     >  1:257180/1‑153 (MQ=255)                                                                                                                                                                                         tAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                   >  1:452672/1‑251 (MQ=255)                                                                                                                                                                                         tAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                   >  1:323908/1‑251 (MQ=255)                                                                                                                                                                                         tAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCaaa                                   >  1:383791/1‑251 (MQ=255)                                                                                                                                                                                           aCCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACAt                                                 >  1:655968/1‑235 (MQ=255)                                                                                                                                                                                                   ctctCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaatt                            >  1:526348/4‑247 (MQ=255)                                                                                                                                                                                                     ctCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat                                                                                                                                                         >  1:91765/2‑114 (MQ=255)                                                                                                                                                                                                     ctCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGAc                                                                                                                                           >  1:628576/2‑137 (MQ=255)                                                                                                                                                                                                      tCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagc                        <  1:496729/251‑8 (MQ=255)                                                                                                                                                                                                       ccAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagca                       >  1:149090/1‑243 (MQ=255)                                                                                                                                                                                                        caatcGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCaattcagcag                      <  1:829310/247‑10 (MQ=255)                                                                                                                                                                                                             gagaTGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAggt                                            >  1:28837/1‑223 (MQ=255)                                                                                                                                                                                                                aTGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATAc                                                                                                                                                    <  1:305969/117‑1 (MQ=255)                                                                                                                                                                                                                  gTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat                                                                                                                                                         >  1:788163/1‑101 (MQ=255)                                                                                                                                                                                                                  gTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCAt                                                                                                         >  1:237478/1‑158 (MQ=255)                                                                                                                                                                                                                    tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg          <  1:846900/251‑4 (MQ=255)                                                                                                                                                                                                                    tGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTATATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaa            >  1:426233/1‑248 (MQ=255)                                                                                                                                                                                                                        tcccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgat      >  1:847545/4‑251 (MQ=255)                                                                                                                                                                                                                         cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTaatg          <  1:664584/244‑4 (MQ=255)                                                                                                                                                                                                                         cccgAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGtgatg     >  1:298321/3‑251 (MQ=255)                                                                                                                                                                                                                             aGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCAACGCCGGCTAAACGTGTTTCAAGGCGTACAACATGAGGTTGTTCCAAACGCAATTCAGCAGTATCTTCTAATGTGATGaca  <  1:803416/250‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACT‑T‑TCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCAACGCCGGCTAAACGCGTTTCCAGGCGTACAACATGAGGCTGTTCCAAACGCAGCTCGGCAGTATCTTCTAGTGTGATGACA  >  1/855458‑855925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑