| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 855,613 | C→T | intergenic (‑5956/‑66605) | unknown ← / → unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 855,613 | 0 | C | T | 97.1% | 82.7 / ‑4.5 | 34 | intergenic (‑5956/‑66605) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Leu‑GAG |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (18/15); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACT‑T‑TCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCA > 1/855401‑855844 | gTTAGTAGTCCGTGATTAATAAACTCGCCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCTTGCAAAACGATTTGACCGTTTTCCATTCCCATAACTTCAGTGATATTCATAATTTTTCTTGAACCATCGTTTAAGCGGGACGCCTGCACTATTATGTTTACTGCTGAAGAAATATTACGGCGAATTGCCTCCAACGGTAGGGATGCATTCGACATCATAAcc < 1:507049/246‑1 (MQ=255) ttACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc < 1:323313/251‑1 (MQ=255) ttACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTCTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAAcc < 1:438528/251‑1 (MQ=255) ttGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCg < 1:105586/251‑1 (MQ=255) ttGCCTTATAAGAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCg < 1:833597/251‑1 (MQ=255) gAAAAAATATCTTGTAAAACAATTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGAccagtgtt > 1:401941/1‑245 (MQ=255) aTTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa < 1:775030/251‑1 (MQ=255) aTTTGCCCATTTTCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa < 1:145787/251‑1 (MQ=255) ccattttCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTtc < 1:390491/248‑1 (MQ=255) tttCCATACCCATTATTTCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTcca > 1:462289/1‑251 (MQ=255) ttccatacccattatttCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTcca > 1:676093/17‑250 (MQ=255) tccatacccattatttCAGTAATATTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCAtc < 1:737220/207‑2 (MQ=255) gtaatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa < 1:846452/218‑1 (MQ=255) gtaatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat > 1:563338/4‑242 (MQ=255) taatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgata < 1:405504/249‑11 (MQ=255) taatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat < 1:629222/248‑10 (MQ=255) taatatTCATGATTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat < 1:418153/248‑10 (MQ=255) tgaTTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCa > 1:782966/3‑251 (MQ=255) gaTTTTTCTTGTTCCATCATTTAAACGGGAGGCTTGTACGATGATATTTACTGCAGATGCAATATTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAg > 1:281034/2‑251 (MQ=255) gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgtag > 1:275003/20‑219 (MQ=255) gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa > 1:689306/20‑251 (MQ=255) gatatttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTaa > 1:215324/20‑251 (MQ=255) atttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGaa > 1:316392/17‑165 (MQ=255) atttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt > 1:743762/17‑216 (MQ=255) ttactgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGCGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCg > 1:28129/15‑251 (MQ=255) ctgcagatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGc > 1:538650/12‑249 (MQ=255) gatgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt > 1:659428/7‑206 (MQ=255) atgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCAtcg > 1:30257/6‑136 (MQ=255) tgcaatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATg > 1:292012/5‑249 (MQ=255) caatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACTCTCTCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTcacctacgat < 1:807965/181‑10 (MQ=255) caatatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACGTAGTGCATTTATTACTAAATCCTGCATAGTAACCTCGCCTGTATGCTCa > 1:117009/3‑251 (MQ=255) atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCAtcgc < 1:12367/134‑4 (MQ=255) atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgtagtgc > 1:872275/1‑200 (MQ=255) atatTACGGCGAATTGCTTCCAACGGTAGGGACGCATTCGACATCATAACCATACT‑C‑TCCAATCGAGATGTTGCATCCCGAGGGGTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCAGTGTTCATCGCTTGTAACATCTGGAATGCTTCTCCACCACGGCATTCACCTACGATAATACGCTCAGGACGCATACgt < 1:776148/201‑2 (MQ=255) | GTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACCGTTTTCCATTCCCATAACCTCAGTGATATTCATAATTTTTCTTGAACCATCATTTAAACGGGACGCCTGCACTATTATGTTTACTGCTGAAGAGATATTACGGCGAATTGCCTCTAACGGTAGGGATGCATTCGACATCATAACCATACT‑T‑TCCAAGCGAGATGTTGCATCGCGAGGGCTATTTGCGTGCAACGTTGACATAGAACCATCGTGACCGGTATTCATTGCTTGTAACATTTGGAATGCTTCTCCACCACGGCATTCGCCCACAATAATACGCTCAGGACGCATACGCAATGCATTTATTACTAAGTCTTGCATAGTAACTTCGCCTGTATGCTCA > 1/855401‑855844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |