Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,388	T→A	intergenic (‑5731/‑66830)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,388	0	T	A	100.0%	13.1 / NA	7	intergenic (‑5731/‑66830)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/5);  total (2/5)

GTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACC  >  1/855198‑855501                                                                                                                                                                                               |                                                                                                                  gttgtcCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTa                                                       >  1:538473/1‑251 (MQ=255)                   gTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGc                                      <  1:818760/250‑1 (MQ=255)                    tATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCAAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCt                                    <  1:699117/251‑1 (MQ=255)                     aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACg                                                  <  1:794038/236‑1 (MQ=255)                     aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCtt                                   >  1:604769/1‑251 (MQ=255)                             cATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGtacttact                                         <  1:549885/237‑1 (MQ=255)                                                      ccAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTgccc  <  1:322659/251‑4 (MQ=255)                                                                                                                                                                                               |                                                                                                                  GTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACC  >  1/855198‑855501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑