Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,367	G→A	intergenic (‑5710/‑66851)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,367	0	G	A	100.0%	15.8 / NA	8	intergenic (‑5710/‑66851)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

GTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACC  >  1/855198‑855501                                                                                                                                                                          |                                                                                                                                       gttgtcCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTa                                                       >  1:538473/1‑251 (MQ=255)                   gTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTgaaaaacagc                                                                                                              >  1:441381/1‑169 (MQ=255)                   gTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGc                                      <  1:818760/250‑1 (MQ=255)                    tATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCAAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCt                                    <  1:699117/251‑1 (MQ=255)                     aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACg                                                  <  1:794038/236‑1 (MQ=255)                     aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCtt                                   >  1:604769/1‑251 (MQ=255)                             cATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGtacttact                                         <  1:549885/237‑1 (MQ=255)                                                      ccAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTgccc  <  1:322659/251‑4 (MQ=255)                                                                                                                                                                          |                                                                                                                                       GTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACC  >  1/855198‑855501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑