Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,293	T→A	intergenic (‑5636/‑66925)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,293	0	T	A	100.0%	37.5 / NA	16	intergenic (‑5636/‑66925)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (7/9);  total (7/9)

ACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTGCGAATCAAGTTTCTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAATTTTTGATTTGAGGCTCTTAAAAAAACTTGTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACC  >  1/855075‑855501                                                                                                                                                                                                                           |                                                                                                                                                                                                                 aCAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcac                                                                                                                                                                                  <  1:795947/251‑11 (MQ=255)                              tataACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcactcagc                                                                                                                                                                             <  1:660140/227‑16 (MQ=255)                                   cAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTaactcagta                                                                                                                                                 >  1:84604/1‑242 (MQ=255)                                     atattacgaactaaGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTaactcagtactc                                                                                                                                              <  1:68201/239‑12 (MQ=255)                                                   gTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcac                                                                                                                                                                                  >  1:289075/1‑191 (MQ=255)                                                                                                           ggttctTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCataatat                                                                                                                                                                                                   >  1:450250/4‑127 (MQ=255)                                                                                                                            gttgtcCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTa                                                       >  1:538473/1‑251 (MQ=255)                                                                                                                               gtccTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGa                                                                                                                                           <  1:274068/161‑1 (MQ=255)                                                                                                                                    aCTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGAc                                                                                                                                                                                                             >  1:356032/1‑93 (MQ=255)                                                                                                                                              gTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTgaaaaacagc                                                                                                              >  1:441381/1‑169 (MQ=255)                                                                                                                                              gTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGc                                      <  1:818760/250‑1 (MQ=255)                                                                                                                                               tATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCAAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCt                                    <  1:699117/251‑1 (MQ=255)                                                                                                                                                aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACg                                                  <  1:794038/236‑1 (MQ=255)                                                                                                                                                aTCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCtt                                   >  1:604769/1‑251 (MQ=255)                                                                                                                                                        cATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGtacttact                                         <  1:549885/237‑1 (MQ=255)                                                                                                                                                                                 ccAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTATCACGGTACTTACTTGCCTTATAAGAAAAAATATCTTGTAAAACAATTTgccc  <  1:322659/251‑4 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                 ACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTGCGAATCAAGTTTCTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAATTTTTGATTTGAGGCTCTTAAAAAAACTTGTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTATCACGATATTTACTTGCCTTATAGGAAAATATATCCTGTAAAACGATTTGACC  >  1/855075‑855501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑