Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 855,211 C→T intergenic (‑5554/‑67007) unknown ← / → unknown tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1855,2110CT100.0% 58.5 / NA 23intergenic (‑5554/‑67007)unknown/unknowntRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

TTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGATCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTGCGAATCAAGTTTCTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAATTTTTGATTTGAGGCTCTTAAAAAAACTTGTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTA  >  1/854976‑855448
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
ttaCGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATagag                                                                                                                                                                                                                                           <  1:420882/240‑1 (MQ=255)
                                       ccAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGt                                                                                                                                                                                         <  1:192908/251‑1 (MQ=255)
                                        caacaaGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGta                                                                                                                                                                                        >  1:657485/1‑251 (MQ=255)
                                                             acacTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATagag                                                                                                                                                                                                                                           >  1:492030/1‑179 (MQ=255)
                                                                       gtCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGtaa                                                                                                                                                            <  1:414867/247‑3 (MQ=255)
                                                                           atatGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATagag                                                                                                                                                                                                                                           >  1:897551/1‑165 (MQ=255)
                                                                           atatGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATaga                                                                                                                                                                                                                                            <  1:604747/164‑1 (MQ=255)
                                                                               gAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATaga                                                                                                                                                                                                                                            <  1:831896/160‑1 (MQ=255)
                                                                                          aCATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATAc                                                                                                                                                                                                                                  <  1:482514/159‑1 (MQ=255)
                                                                                          aCATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGtatat                                                                                                                                                                                     >  1:299468/1‑204 (MQ=255)
                                                                                                   aCAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcac                                                                                                                             <  1:795947/251‑11 (MQ=255)
                                                                                                                             acTTATAACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGt                                                                                                                                                                                         >  1:431997/1‑165 (MQ=255)
                                                                                                                                tataACAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcactcagc                                                                                                                        <  1:660140/227‑16 (MQ=255)
                                                                                                                                     cAATATTACGAACTAAGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTaactcagta                                                                                            >  1:84604/1‑242 (MQ=255)
                                                                                                                                       atattacgaactaaGTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTaactcagtactc                                                                                         <  1:68201/239‑12 (MQ=255)
                                                                                                                                                     gTTTTTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAaataatggctcatcac                                                                                                                             >  1:289075/1‑191 (MQ=255)
                                                                                                                                                                     ttAGTAAAGTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATaga                                                                                                                                                                                                                                            <  1:99304/74‑1 (MQ=255)
                                                                                                                                                                             gTGATAATACCATAAACTAACTTTTGATTTGAGGTTCTTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATataaa                                                                                                                                                                                <  1:501423/126‑1 (MQ=255)
                                                                                                                                                                                                             ggttctTAAAAAAACTTGTTGTCCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCataatat                                                                                                                                              >  1:450250/4‑127 (MQ=255)
                                                                                                                                                                                                                              gttgtcCTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGATTAAATACTTGAGCATTTTGGAAAACAGCAGAGCGAGTTAATAAACCATGGTTAACAAATTCACCAATAATTTTGCCATCTTTa  >  1:538473/1‑251 (MQ=255)
                                                                                                                                                                                                                                 gtccTACTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGACGGCATAATATAAATAATGGCTCATCACTCAGCTTCTCCGAAAATACTTTGTAACTCAGTACTCAGa                                                                                      <  1:274068/161‑1 (MQ=255)
                                                                                                                                                                                                                                      aCTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGTATATATAAATAATAGTCCACCTATTGTAAGGAc                                                                                                                                                        >  1:356032/1‑93 (MQ=255)
                                                                                                                                                                                                                                       cTTATAGAGGTATCATACTCATTAATTTTTCTTTTAGTAGAAAACCAACTACTTAAGGt                                                                                                                                                                                         <  1:412416/59‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
TTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGATCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATTGCGAATCAAGTTTCTTTTATCACCTTTAGTAAAGTGATAATACCATAAACTAATTTTTGATTTGAGGCTCTTAAAAAAACTTGTTGTTCTACTTACAGAGGTATCATACTCATTAATTTTTCTTTTAGTGGAAAACCAACTACTTAAGGTATATATAAATAACAGCCCACCTATTGTTAGGACGGCATAATACAAATAATAATTCATTACTCAGCTTCTCCGAAGATACTTTGTAGTTCAGCACTCAGATTGAATACTTGAGCATTTTGGAATACAACAGAACGAGTTAGTAGTCCGTGATTAATAAACTCACCAAGAATTTTACCCTCTTTA  >  1/854976‑855448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑