Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,909	A→G	intergenic (‑5252/‑67309)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,909	0	A	G	100.0%	116.6 / NA	43	intergenic (‑5252/‑67309)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

TAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGATCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATT  >  1/854695‑855115                                                                                                                                                                                                                       |                                                                                                                                                                                                               tAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTAcc                                                                                                                                                                            <  1:842261/251‑1 (MQ=255)      tcccgTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTctgg                                                                                                                                                                       >  1:393986/4‑248 (MQ=255)                          gtttaaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttt                                                                                                                                                   >  1:351924/11‑251 (MQ=255)                              aaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttttttt                                                                                                                                               >  1:678777/7‑251 (MQ=255)                                      gCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGa                                                                                                                                                                      >  1:408478/1‑220 (MQ=255)                                        gTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTa                                                                                                                                      >  1:271444/1‑250 (MQ=255)                                         tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     <  1:290066/250‑1 (MQ=255)                                         tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     <  1:579626/250‑1 (MQ=255)                                             aTAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCATCGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc                                                                                                                                <  1:238451/251‑1 (MQ=255)                                                gTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTttat                                                                                                                                             <  1:549062/235‑1 (MQ=255)                                                                 ctCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     <  1:617992/225‑1 (MQ=255)                                                                  tCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATaaacaaaac                                                                                                           <  1:816286/251‑1 (MQ=255)                                                                   ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     <  1:771087/224‑1 (MQ=255)                                                                   ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     >  1:513144/1‑224 (MQ=255)                                                                   ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc                                                                                                                                >  1:482687/1‑229 (MQ=255)                                                                                                                ggtcctcccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATg                                                              <  1:604411/226‑1 (MQ=255)                                                                                                                ggtcctcccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATg                                                              <  1:251184/226‑1 (MQ=255)                                                                                                                   cctcccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATg                                                              >  1:871446/22‑247 (MQ=255)                                                                                                                   cctcccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAata                                                          <  1:281931/230‑1 (MQ=255)                                                                                                                   cctcccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAata                                                          <  1:691957/230‑1 (MQ=255)                                                                                                                      cccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAacac                                                                             <  1:499845/211‑1 (MQ=255)                                                                                                                      cccctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATaa                                                       >  1:567090/19‑251 (MQ=255)                                                                                                                        cctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     >  1:335149/17‑171 (MQ=255)                                                                                                                        cctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATc                                                                        >  1:1051/17‑232 (MQ=255)                                                                                                                         ctatagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATaaacaaaac                                                                                                           <  1:504647/181‑1 (MQ=255)                                                                                                                           atagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATc                                                                        <  1:515490/216‑1 (MQ=255)                                                                                                                           atagagaggcgtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGc                                                     <  1:597387/235‑1 (MQ=255)                                                                                                                                     gtctATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAacac                                                                             >  1:622235/4‑214 (MQ=255)                                                                                                                                        tATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATg                                                              >  1:109575/1‑226 (MQ=255)                                                                                                                                         atgaaTACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATg                                                              >  1:582068/5‑225 (MQ=255)                                                                                                                                                             cgccTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATc                                                                        >  1:142468/3‑195 (MQ=255)                                                                                                                                                                cTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACtt             <  1:861787/251‑1 (MQ=255)                                                                                                                                                                 tAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATg                                                              >  1:3738/1‑201 (MQ=255)                                                                                                                                                                     tGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAAc        <  1:405534/251‑1 (MQ=255)                                                                                                                                                                     tGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAAc        <  1:288970/251‑1 (MQ=255)                                                                                                                                                                           gttaGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCcaaca                                                                                                 <  1:391947/153‑1 (MQ=255)                                                                                                                                                                           gttaGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGaa                                                            >  1:778425/4‑193 (MQ=255)                                                                                                                                                                           gttaGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATAtt  >  1:742711/4‑251 (MQ=255)                                                                                                                                                                           gttaGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATAtt  >  1:227566/4‑251 (MQ=255)                                                                                                                                                                                    ttACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                                                                                                                                     <  1:12792/111‑1 (MQ=255)                                                                                                                                                                                     tACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAaac                  >  1:604861/1‑225 (MQ=255)                                                                                                                                                                                       ccGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGGTCAATATGAata                                                          >  1:263360/1‑183 (MQ=255)                                                                                                                                                                                              gTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATaaacaaaac                                                                                                           <  1:375250/127‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                               TAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAACAAATCCAACAAGAAAAATTAAGCAAAACACTAATCGATCAATATGAATATAAGCAACATTAATTACAAAAAAAGCAAAGAAAATAAAACAACTTATAACAATATT  >  1/854695‑855115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑