Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,800	A→T	intergenic (‑5143/‑67418)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,800	0	A	T	100.0%	49.9 / NA	19	intergenic (‑5143/‑67418)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/11);  total (8/11)

CCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAAC  >  1/854623‑855010                                                                                                                                                                                  |                                                                                                                                                                                                                   ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATcc                                                                                                                                           <  1:38079/251‑1 (MQ=255)                                 aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                                                                                           <  1:213886/251‑1 (MQ=255)                                 aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAAATCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                                                                                           <  1:573281/251‑1 (MQ=255)                                                                      aaaTAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGAcctgtta                                                                                                                                                >  1:769686/1‑172 (MQ=255)                                                                         tAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTAcc                                                                   <  1:842261/251‑1 (MQ=255)                                                                              tcccgTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTctgg                                                              >  1:393986/4‑248 (MQ=255)                                                                                                  gtttaaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttt                                          >  1:351924/11‑251 (MQ=255)                                                                                                      aaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttttttt                                      >  1:678777/7‑251 (MQ=255)                                                                                                              gCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGa                                                             >  1:408478/1‑220 (MQ=255)                                                                                                                gTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTa                             >  1:271444/1‑250 (MQ=255)                                                                                                                 tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:290066/250‑1 (MQ=255)                                                                                                                 tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:579626/250‑1 (MQ=255)                                                                                                                     aTAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCATCGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc                       <  1:238451/251‑1 (MQ=255)                                                                                                                        gTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTttat                                    <  1:549062/235‑1 (MQ=255)                                                                                                                                         ctCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:617992/225‑1 (MQ=255)                                                                                                                                          tCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATaaacaaaac  <  1:816286/251‑1 (MQ=255)                                                                                                                                           ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            >  1:513144/1‑224 (MQ=255)                                                                                                                                           ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:771087/224‑1 (MQ=255)                                                                                                                                           ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc                       >  1:482687/1‑229 (MQ=255)                                                                                                                                                                                  |                                                                                                                                                                                                                   CCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAAC  >  1/854623‑855010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑