Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,789	T→G	intergenic (‑5132/‑67429)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,789	0	T	G	100.0%	68.2 / NA	26	intergenic (‑5132/‑67429)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/15);  total (11/15)

TTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATTATAGCTGACATTCTAGCCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAAC  >  1/854548‑855010                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                              tttCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCaaaaa                                                                                                                                                                                                                        >  1:800048/1‑249 (MQ=255)       gCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcg                                                                                                                                                                                                                >  1:319564/1‑246 (MQ=255)         aTAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggg                                                                                                                                                                                                              >  1:419918/1‑244 (MQ=255)                       aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                                                                                <  1:12717/251‑22 (MQ=255)                       aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                                                                                <  1:89796/251‑22 (MQ=255)                       aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                                                                                <  1:262085/251‑22 (MQ=255)                           aaaaagaaaCCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatagag                                                                                                                                                                                              <  1:758549/249‑24 (MQ=255)                                                                            ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATcc                                                                                                                                           <  1:38079/251‑1 (MQ=255)                                                                                                            aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                                                                                           <  1:213886/251‑1 (MQ=255)                                                                                                            aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAAATCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                                                                                           <  1:573281/251‑1 (MQ=255)                                                                                                                                                 aaaTAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGAcctgtta                                                                                                                                                >  1:769686/1‑172 (MQ=255)                                                                                                                                                    tAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTAcc                                                                   <  1:842261/251‑1 (MQ=255)                                                                                                                                                         tcccgTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTctgg                                                              >  1:393986/4‑248 (MQ=255)                                                                                                                                                                             gtttaaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttt                                          >  1:351924/11‑251 (MQ=255)                                                                                                                                                                                 aaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttttttt                                      >  1:678777/7‑251 (MQ=255)                                                                                                                                                                                         gCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGa                                                             >  1:408478/1‑220 (MQ=255)                                                                                                                                                                                           gTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTa                             >  1:271444/1‑250 (MQ=255)                                                                                                                                                                                            tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:579626/250‑1 (MQ=255)                                                                                                                                                                                            tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:290066/250‑1 (MQ=255)                                                                                                                                                                                                aTAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCATCGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc                       <  1:238451/251‑1 (MQ=255)                                                                                                                                                                                                   gTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTttat                                    <  1:549062/235‑1 (MQ=255)                                                                                                                                                                                                                    ctCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:617992/225‑1 (MQ=255)                                                                                                                                                                                                                     tCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATaaacaaaac  <  1:816286/251‑1 (MQ=255)                                                                                                                                                                                                                      ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            <  1:771087/224‑1 (MQ=255)                                                                                                                                                                                                                      ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc                            >  1:513144/1‑224 (MQ=255)                                                                                                                                                                                                                      ccTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc                       >  1:482687/1‑229 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                              TTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATTATAGCTGACATTCTAGCCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACCAAGTTTCCAAATAAACAAAAC  >  1/854548‑855010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑