Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,750	G→A	intergenic (‑5093/‑67468)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,750	0	G	A	100.0%	94.4 / NA	36	intergenic (‑5093/‑67468)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/19);  total (17/19)

CGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATTATAGCTGACATTCTAGCCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACC  >  1/854518‑854989                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                cGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTatat                                                                                                                                                                                                                               >  1:753931/1‑251 (MQ=255)        gTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTatat                                                                                                                                                                                                                               <  1:344959/244‑1 (MQ=255)           attaattaAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGAt                                                                                                                                                                                                                     <  1:638598/251‑1 (MQ=255)              aattaaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATAAGGGTAACGATCAt                                                                                                                                                                                                                  <  1:159390/251‑1 (MQ=255)                ttaaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAg                                                                                                                                                                                                                <  1:252842/251‑1 (MQ=255)                 taaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGc                                                                                                                                                                                                               <  1:894751/251‑1 (MQ=255)                  aaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCt                                                                                                                                                                                                              <  1:122466/251‑1 (MQ=255)                               tttCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCaaaaa                                                                                                                                                                                                   >  1:800048/1‑249 (MQ=255)                                     gCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcg                                                                                                                                                                                           >  1:319564/1‑246 (MQ=255)                                       aTAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggg                                                                                                                                                                                         >  1:419918/1‑244 (MQ=255)                                       aTAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGCCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCta                                                                                                                                                                                                             >  1:666186/1‑230 (MQ=255)                                                     aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                                                           <  1:12717/251‑22 (MQ=255)                                                     aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                                                           <  1:89796/251‑22 (MQ=255)                                                     aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                                                           <  1:262085/251‑22 (MQ=255)                                                         aaaaagaaaCCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatagag                                                                                                                                                                         <  1:758549/249‑24 (MQ=255)                                                                            aaCATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATgg                                                                                                                                                                                                                             >  1:881776/1‑178 (MQ=255)                                                                             acatgCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTtata                                                                                                                                                                                                                                >  1:483872/5‑174 (MQ=255)                                                                              catgCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATg                                                                                                                                                                                                                              >  1:831937/4‑175 (MQ=255)                                                                                tgCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTatat                                                                                                                                                                                                                               >  1:810491/2‑172 (MQ=255)                                                                                                          ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATg                                                                                                                                                                                                                              >  1:413460/1‑147 (MQ=255)                                                                                                          ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATg                                                                                                                                                                                                                              <  1:267094/147‑1 (MQ=255)                                                                                                          ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATcc                                                                                                                      <  1:38079/251‑1 (MQ=255)                                                                                                                                          aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                                                                      <  1:213886/251‑1 (MQ=255)                                                                                                                                          aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAAATCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                                                                      <  1:573281/251‑1 (MQ=255)                                                                                                                                             ttATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCt                                                                                                                                                                                                              >  1:844472/1‑128 (MQ=255)                                                                                                                                                                               aaaTAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGAcctgtta                                                                                                                           >  1:769686/1‑172 (MQ=255)                                                                                                                                                                                  tAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTAcc                                              <  1:842261/251‑1 (MQ=255)                                                                                                                                                                                       tcccgTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTctgg                                         >  1:393986/4‑248 (MQ=255)                                                                                                                                                                                                           gtttaaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttt                     >  1:351924/11‑251 (MQ=255)                                                                                                                                                                                                               aaattagTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGttttttt                 >  1:678777/7‑251 (MQ=255)                                                                                                                                                                                                                       gCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGa                                        >  1:408478/1‑220 (MQ=255)                                                                                                                                                                                                                         gTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTa        >  1:271444/1‑250 (MQ=255)                                                                                                                                                                                                                          tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc       <  1:290066/250‑1 (MQ=255)                                                                                                                                                                                                                          tACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTAc       <  1:579626/250‑1 (MQ=255)                                                                                                                                                                                                                              aTAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCATCGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGAcc  <  1:238451/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTCTGGAAACATTGCGTTGAATGTTTTTttat               <  1:549062/235‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                CGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATTATAGCTGACATTCTAGCCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGGAAACATTGCGTTGAATGTTTTTTTATTACGGTACTGACC  >  1/854518‑854989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑