Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,714	G→A	intergenic (‑5057/‑67504)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,714	0	G	A	100.0%	146.6 / NA	54	intergenic (‑5057/‑67504)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/31);  total (23/31)

TCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATTATAGCTGACATTCTAGCCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGG  >  1/854468‑854950                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             tCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATCATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtt                                                                                                                                                                                                                                          <  1:568092/251‑1 (MQ=255)   ccATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttg                                                                                                                                                                                                                                        <  1:264799/251‑2 (MQ=255)           aaaaGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:406210/251‑10 (MQ=255)           aaaaGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:869277/251‑10 (MQ=255)           aaaaGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:379835/251‑10 (MQ=255)               gctCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaattagtg                                                                                                                                                                                                                            >  1:23726/3‑238 (MQ=255)                ctCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaattagtg                                                                                                                                                                                                                            <  1:165723/249‑14 (MQ=255)                      accAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:394928/239‑10 (MQ=255)                      accAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACaa                                                                                                                                                                                                                     >  1:48413/2‑251 (MQ=255)                      accAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACaa                                                                                                                                                                                                                     >  1:30189/2‑251 (MQ=255)                         aaaaaCATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAg                                                                                                                                                                                                                  <  1:455380/251‑1 (MQ=255)                         aaaaaCATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAg                                                                                                                                                                                                                  <  1:428846/251‑1 (MQ=255)                         aaaaaCATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAg                                                                                                                                                                                                                  <  1:434424/251‑1 (MQ=255)                         aaaaaCATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAg                                                                                                                                                                                                                  <  1:858964/251‑1 (MQ=255)                         aaaaaCATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAg                                                                                                                                                                                                                  <  1:493698/251‑1 (MQ=255)                           aaaCATAATAAAGCACGTATCTACCGGTAGGGCCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGtt                                                                                                                                                                                                                >  1:416822/1‑251 (MQ=255)                             aCATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:3581/233‑10 (MQ=255)                              cATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAAt                                                                                                                                                                                                                    >  1:198605/1‑244 (MQ=255)                              cATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATg                                                                                                                                                                                                             >  1:414653/1‑251 (MQ=255)                                       gCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTa                                                                                                                                                                                                               >  1:829197/1‑240 (MQ=255)                                          cGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACACGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATgaaaaagtaaaa                                                                                                                                                                                                  >  1:427062/1‑241 (MQ=255)                                              tCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATgaaaaagtaaaactc                                                                                                                                                                                               >  1:476528/1‑237 (MQ=255)                                                   cGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTatat                                                                                                                                                                                        >  1:753931/1‑251 (MQ=255)                                                          gTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTatat                                                                                                                                                                                        <  1:344959/244‑1 (MQ=255)                                                             attaattaAGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGAt                                                                                                                                                                              <  1:638598/251‑1 (MQ=255)                                                                aattaaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATAAGGGTAACGATCAt                                                                                                                                                                           <  1:159390/251‑1 (MQ=255)                                                                  ttaaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAg                                                                                                                                                                         <  1:252842/251‑1 (MQ=255)                                                                   taaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGc                                                                                                                                                                        <  1:894751/251‑1 (MQ=255)                                                                    aaGAAATCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCt                                                                                                                                                                       <  1:122466/251‑1 (MQ=255)                                                                       aaaTCAAAGTTTTCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAg                                                                                                                                                                                                                  >  1:880998/1‑205 (MQ=255)                                                                                 tttCCGGCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCaaaaa                                                                                                                                                            >  1:800048/1‑249 (MQ=255)                                                                                       gCATAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcg                                                                                                                                                    >  1:319564/1‑246 (MQ=255)                                                                                         aTAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggg                                                                                                                                                  >  1:419918/1‑244 (MQ=255)                                                                                         aTAGCAAACTGCATAAAGAAAAAGAAACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGCCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCta                                                                                                                                                                      >  1:666186/1‑230 (MQ=255)                                                                                                       aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                    <  1:89796/251‑22 (MQ=255)                                                                                                       aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                    <  1:12717/251‑22 (MQ=255)                                                                                                       aaagaaaaagaaACCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatag                                                                                                                                    <  1:262085/251‑22 (MQ=255)                                                                                                           aaaaagaaaCCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTgtagcggggtcctcccctatagag                                                                                                                                  <  1:758549/249‑24 (MQ=255)                                                                                                            aaaagaaaCCGACCGGAAAACATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACaa                                                                                                                                                                                                                     <  1:818790/165‑1 (MQ=255)                                                                                                                              aaCATGCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATgg                                                                                                                                                                                      >  1:881776/1‑178 (MQ=255)                                                                                                                               acatgCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTtata                                                                                                                                                                                         >  1:483872/5‑174 (MQ=255)                                                                                                                                catgCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATg                                                                                                                                                                                       >  1:831937/4‑175 (MQ=255)                                                                                                                                  tgCTACGATTATAGCTGACATTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTatat                                                                                                                                                                                        >  1:810491/2‑172 (MQ=255)                                                                                                                                                     aTTCTGGCCTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:75285/113‑10 (MQ=255)                                                                                                                                                            ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCtttgtttaaatt                                                                                                                                                                                                                                <  1:842338/106‑10 (MQ=255)                                                                                                                                                            ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATg                                                                                                                                                                                       <  1:267094/147‑1 (MQ=255)                                                                                                                                                            ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATg                                                                                                                                                                                       >  1:413460/1‑147 (MQ=255)                                                                                                                                                            ccTCTGATGTCATTGCTTTCTTTTTCTTTTCCATTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATcc                                                                               <  1:38079/251‑1 (MQ=255)                                                                                                                                                                                            aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                               <  1:213886/251‑1 (MQ=255)                                                                                                                                                                                            aTTTTATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAAATCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGcc                                               <  1:573281/251‑1 (MQ=255)                                                                                                                                                                                               ttATTGCTATCCGCAATTACGCGACCTAAACGAGAAATAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCt                                                                                                                                                                       >  1:844472/1‑128 (MQ=255)                                                                                                                                                                                                                                 aaaTAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGAcctgtta                                                                                    >  1:769686/1‑172 (MQ=255)                                                                                                                                                                                                                                    tAACCTCCCGTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTAcc       <  1:842261/251‑1 (MQ=255)                                                                                                                                                                                                                                         tcccgTATCTGTCCACCTTTGTTTAAATTAGTGCGTACAATAGTTATGAAAAAGTAAAACTCCTTATATGGGTAACGATCATAGCTATCGTCAAAAACTGTAGCGGGGTCCTCCCCTATAGAGAGGCGTCTATGAATACTTTTGAACTCCTCGCCTAATTGACCTGTTAGATCCTTACCGCAACGTTCCAATGCCTGTAATAAGCCTACGCTAGAAGTTGCTGCAGCATTGAGAATCTGAATTACCTctgg  >  1:393986/4‑248 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             TCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAATTGCATAAAGAAAAAGAAACCGACAGGAAAACACGCTACAATTATAGCTGACATTCTAGCCTCTGATGTCATTGCTTTCTTTTTCTTCTCCATTTTATTGCTATCGGCAATTACACGACCTAAACGGGAAATAACCTCGCGTATCTGTCCGCCTTTACTTAAGTTGGTGCGTACAATAGTTATGAAGAAATAGAATTCCTTATATGGGTAACGATCATAGCTGTCTTCAAAAACTGAAACAGGATCCTCGCCTATTGCAATACGCTTATGGATACTCTTAAACTCCTCACCTAATTGACCGGTAAGATCCTTACCGCAGCGTTCTAATGCTTGTAATAAGCCAGCACCTGAAGTTGCCGCTGCATTGAGAATCTGAATTACCTCCGG  >  1/854468‑854950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑