Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,333	C→T	intergenic (‑4676/‑67885)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,333	0	C	T	100.0%	72.8 / NA	28	intergenic (‑4676/‑67885)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

GTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTTTAATCATCATGTTGACTTCCTCATCAACCACCAAATGATTCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAA  >  1/854089‑854563                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       gTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTcttt                                                                                                                                                                                                                                  >  1:282703/1‑248 (MQ=255)                                    aGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCACTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGcca                                                                                                                                                                                               >  1:643794/1‑249 (MQ=255)                                          atcaATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaata                                                                                                                                                                                         >  1:806403/1‑243 (MQ=255)                                                                             gtaaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATaa                                                                                                                                                      >  1:347992/18‑251 (MQ=255)                                                                              taaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAg                                                                                                                                                     <  1:761644/235‑1 (MQ=255)                                                                                      taatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGacagtaat                                                                                                                                             <  1:741339/243‑8 (MQ=255)                                                                                            gtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaataatac                                                                                                                                                                                     <  1:738420/203‑13 (MQ=255)                                                                                             taaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgct                                                                                                                                         <  1:170225/247‑3 (MQ=255)                                                                                                                    cAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCtatgc                                                                                                                                                                                                                           <  1:438670/143‑3 (MQ=255)                                                                                                                                     tttaaaacgggATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAGTTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTccc                                                                                              <  1:889899/242‑1 (MQ=255)                                                                                                                                              ggATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGt                                                                                                                             <  1:318174/211‑1 (MQ=255)                                                                                                                                                   attgttaatcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgc                                                                                                                                          <  1:177660/189‑2 (MQ=255)                                                                                                                                                   attgttaatcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAAt                                                                                                     <  1:258979/226‑1 (MQ=255)                                                                                                                                                          atcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGac                                                                                                                                                   >  1:299930/4‑176 (MQ=255)                                                                                                                                                          atcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCa                                                                         >  1:343804/4‑251 (MQ=255)                                                                                                                                                              gtaacagctcaagttcaaTTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATAc                                                                                          >  1:877369/9‑230 (MQ=255)                                                                                                                                                                          gttcaaTTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCa                                                         >  1:460298/1‑251 (MQ=255)                                                                                                                                                                                                     tggctatttcatcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCattaatta                              >  1:194400/20‑251 (MQ=255)                                                                                                                                                                                                      ggctatttcatcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTc                                                                                                <  1:172888/166‑1 (MQ=255)                                                                                                                                                                                                              catcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATaa                                                                                                                                                             >  1:465881/11‑114 (MQ=255)                                                                                                                                                                                                                tcattactttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAAc                                                                    <  1:791350/194‑1 (MQ=255)                                                                                                                                                                                                                     actttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAAGTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGt                                            <  1:439354/218‑1 (MQ=255)                                                                                                                                                                                                                      ctttGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGac                                                                                                                                                   <  1:143458/115‑2 (MQ=255)                                                                                                                                                                                                                                tAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCaa   <  1:746596/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCaaa  >  1:279608/1‑249 (MQ=255)                                                                                                                                                                                                                                         ctcCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAAt                                                                                                     <  1:637747/144‑1 (MQ=255)                                                                                                                                                                                                                                              gTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTAc                                                >  1:163611/1‑192 (MQ=255)                                                                                                                                                                                                                                              gTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGt                  >  1:719046/1‑222 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       GTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTTTAATCATCATGTTGACTTCCTCATCAACCACCAAATGATTCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCACGTATCTACCGGTAGGGTCATTAATTAAGAAATCAAAGTTTTCCGGCATAGCAAA  >  1/854089‑854563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑