Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,299	C→T	intergenic (‑4642/‑67919)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,299	0	C	T	94.7%	39.9 / ‑4.2	19	intergenic (‑4642/‑67919)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (9/9);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTTTAATCATCATGTTGACTTCCTCATCAACCACCAAATGATTCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCA  >  1/854062‑854508                                                                                                                                                                                                                                              |                                                                                                                                                                                                                  ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCtt                                                                                                                                                                                                      >  1:92602/1‑251 (MQ=255)                 ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTtctc                                                                                                                                                                                           >  1:611199/2‑246 (MQ=255)                            gTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTcttt                                                                                                                                                                           >  1:282703/1‑248 (MQ=255)                                                               aGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCACTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGcca                                                                                                                                        >  1:643794/1‑249 (MQ=255)                                                                     atcaATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaata                                                                                                                                  >  1:806403/1‑243 (MQ=255)                                                                                                        gtaaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATaa                                                                                               >  1:347992/18‑251 (MQ=255)                                                                                                         taaaaccataatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAg                                                                                              <  1:761644/235‑1 (MQ=255)                                                                                                                 taatcagtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGacagtaat                                                                                      <  1:741339/243‑8 (MQ=255)                                                                                                                       gtaaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaataatac                                                                                                                              <  1:738420/203‑13 (MQ=255)                                                                                                                        taaTTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgct                                                                                  <  1:170225/247‑3 (MQ=255)                                                                                                                                               cAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCtatgc                                                                                                                                                                    <  1:438670/143‑3 (MQ=255)                                                                                                                                                                tttaaaacgggATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAGTTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTccc                                       <  1:889899/242‑1 (MQ=255)                                                                                                                                                                         ggATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGt                                                                      <  1:318174/211‑1 (MQ=255)                                                                                                                                                                              attgttaatcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAgc                                                                                   <  1:177660/189‑2 (MQ=255)                                                                                                                                                                              attgttaatcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAAt                                              <  1:258979/226‑1 (MQ=255)                                                                                                                                                                                     atcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGac                                                                                            >  1:299930/4‑176 (MQ=255)                                                                                                                                                                                     atcaGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCa                  >  1:343804/4‑251 (MQ=255)                                                                                                                                                                                         gtaacagctcaagttcaaTTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATAc                                   >  1:877369/9‑230 (MQ=255)                                                                                                                                                                                                     gttcaaTTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGCCAAAAATAATACAAACACACCAAAAATGGTTAATAATGCATAAGACAGTAATAGCTTAATTATCATGTTGACTTCCTCATCAACCACCAAATAATTCCCATACCGAAAAGCTCACTACCAAAAACATAATAAAGCa  >  1:460298/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                  GGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACAATACAATAATACCAAAAATCGTTAATAGCGCATAAGATAATAATAGTTTAATCATCATGTTGACTTCCTCATCAACCACCAAATGATTCCCATACCGAAAAGTTCACTGCCAAAAACATAATAAAGCA  >  1/854062‑854508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑