Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,138	A→G	intergenic (‑4481/‑68080)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,138	0	A	G	100.0%	17.7 / NA	8	intergenic (‑4481/‑68080)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/2);  total (6/2)

TTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACA  >  1/853898‑854380                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   tcaGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAg                                                                                                                                                                                                                                           <  1:432260/248‑1 (MQ=255)                                                                                                                                                                     ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCtt                                                                      >  1:92602/1‑251 (MQ=255)                                                                                                                                                                                     ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTacctttggctatttcatcatt                                                                                  >  1:450042/2‑203 (MQ=255)                                                                                                                                                                                     ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTtctc                                                           >  1:611199/2‑246 (MQ=255)                                                                                                                                                                                                gTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTcttt                                           >  1:282703/1‑248 (MQ=255)                                                                                                                                                                                                                   gagaTGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAAtt                                                                                                                     <  1:764065/158‑1 (MQ=255)                                                                                                                                                                                                                                   aGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCACTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGcca        >  1:643794/1‑249 (MQ=255)                                                                                                                                                                                                                                         atcaATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTCTTTCCTATGCTGGAATTTTCTTCTAGCTGAAAGTGccaaaaata  >  1:806403/1‑243 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   TTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTTTCTATATTGGAATTTTTTTCTGGCTGAAAGTGCGGCAAACA  >  1/853898‑854380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑