Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,115	A→G	intergenic (‑4458/‑68103)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,115	0	A	G	100.0%	27.0 / NA	11	intergenic (‑4458/‑68103)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/5);  total (6/5)

CGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTT  >  1/853873‑854339                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 cGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTcagt                                                                                                                                                                                                                          <  1:205908/251‑4 (MQ=255)         aCGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTcagt                                                                                                                                                                                                                          >  1:113262/1‑240 (MQ=255)                ataacataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATaag                                                                                                                                                                                                           <  1:527838/251‑2 (MQ=255)                          tcaGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAg                                                                                                                                                                                                  <  1:432260/248‑1 (MQ=255)                                                                                                                                                                                              ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATaag                                                                                                                                                                                                           >  1:478461/1‑76 (MQ=255)                                                                                                                                                                                              ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCtt                             >  1:92602/1‑251 (MQ=255)                                                                                                                                                                                                              ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGttt                                                                                                                                                                                                                     <  1:439445/50‑1 (MQ=255)                                                                                                                                                                                                              ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTacctttggctatttcatcatt                                         >  1:450042/2‑203 (MQ=255)                                                                                                                                                                                                              ctattaATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTtctc                  >  1:611199/2‑246 (MQ=255)                                                                                                                                                                                                                         gTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCTTAGGTCTTTCTCCAGTAATAATTTcttt  >  1:282703/1‑248 (MQ=255)                                                                                                                                                                                                                                            gagaTGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAAtt                                                                            <  1:764065/158‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 CGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTTAGGTCTTTCTCCAGTAATAACTTCCTT  >  1/853873‑854339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑