Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,067	G→A	intergenic (‑4410/‑68151)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,067	0	G	A	100.0%	44.0 / NA	18	intergenic (‑4410/‑68151)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

GAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTT  >  1/853828‑854312                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      gagagaTCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGtta                                                                                                                                                                                                                                             <  1:795950/250‑1 (MQ=255)     gaTCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGttactatt                                                                                                                                                                                                                                        >  1:44947/1‑251 (MQ=255)         ctgcaaTGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGGCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAata                                                                                                                                                                                                                                    <  1:612008/247‑1 (MQ=255)                               gTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                                                                                                                                                                                                   >  1:70082/1‑230 (MQ=255)                                     tcggATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATGTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGa                                                                                                                                                                                                        <  1:694927/248‑1 (MQ=255)                                              cGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTcagt                                                                                                                                                                                               <  1:205908/251‑4 (MQ=255)                                                    ataCGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGttactatt                                                                                                                                                                                                                                        <  1:662688/204‑1 (MQ=255)                                                      aCGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTcagt                                                                                                                                                                                               >  1:113262/1‑240 (MQ=255)                                                           acataacataAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                                                                                                                                                                                                   >  1:254665/1‑202 (MQ=255)                                                           acataacataAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                                                                                                                                                                                                   <  1:578923/202‑1 (MQ=255)                                                             ataacataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATaag                                                                                                                                                                                <  1:527838/251‑2 (MQ=255)                                                              taacataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAg                                                                                                                                                                                                                                                <  1:320163/186‑1 (MQ=255)                                                                acataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAata                                                                                                                                                                                                                                    >  1:341250/1‑196 (MQ=255)                                                                       tcaGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAg                                                                                                                                                                       <  1:432260/248‑1 (MQ=255)                                                                               gggtttAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                                                                                                                                                                                                   >  1:34736/4‑182 (MQ=255)                                                                                                                                               gtaaaTTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAAtata                                                                                                                                                                                                                                  >  1:424962/5‑119 (MQ=255)                                                                                                                                                                                                                                           ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATaag                                                                                                                                                                                >  1:478461/1‑76 (MQ=255)                                                                                                                                                                                                                                           ggAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAGATAATAAACCCCGCAAAGTAAAACCATAATCAGTAATTTTACTTTTATATTTTTATCAATAATTCCAAGCATTTTTAAAACGGGATTATTGTTAATCAGTAACAGCTCAAGTTCAATTTGCTGCTTGCTTTTACCTTTGGCTATTTCATCATTACTTTGATCCtt  >  1:92602/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      GAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAGATAGTAAACTCCAAAAAGCAAAAGCATGATCAACAATTTTACTTTTATATTTTTATCAATAATTCCGAGTACTTTTAAGATAGGATTATTATTAATTAGTAATAGTTCAAGTTCAATTTGCTGTTTGCTCTTATTTTTAGCTACTTCGTCACTATCTTGATCCTT  >  1/853828‑854312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑