Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,908	A→G	intergenic (‑4251/‑68310)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,908	0	A	G	100.0%	102.7 / NA	37	intergenic (‑4251/‑68310)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/27);  total (10/27)

GCGTACCAAGCTTTTCTTCTATAGCTAATAATTGAATTTCTCTAATATCAGTCGATAGTTGAATTAATTGATGATAAATAGAGGAACCAAAATTCAGACTTTGCTGCATTACAGTGCAAAACATCCTGATTTCCGTTGTTGGTAAAGACACAGAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAG  >  1/853676‑854147                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                                                                                                                                                                         <  1:748897/239‑3 (MQ=255)   gTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGATATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                                                                                                                                                                         <  1:344678/239‑3 (MQ=255)   gTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAt                                                                                                                                                                                                                                           >  1:644185/1‑237 (MQ=255)     aCCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAg                                                                                                                                                                                                                                  <  1:566096/244‑1 (MQ=255)        aagtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                                                                                                                                                                         <  1:717775/230‑3 (MQ=255)                     ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                                                                                                                                                                           <  1:238065/251‑4 (MQ=255)                     ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                                                                                                                                                                           <  1:723457/251‑4 (MQ=255)                     ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                                                                                                                                                                           <  1:49593/251‑4 (MQ=255)                                                             gaactagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAcccattcccg                                                                                                                                                                                       <  1:158094/224‑10 (MQ=255)                                                                 tagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAAt                                                                                                                                                               <  1:510932/248‑1 (MQ=255)                                                                     tgatgaTAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAAt                                                                                                                                                           <  1:699177/251‑1 (MQ=255)                                                                                 gagGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                                                                                                                                                                         >  1:690597/1‑159 (MQ=255)                                                                                                             ttACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCaa                                                                                                                                                                                                                        <  1:597789/150‑1 (MQ=255)                                                                                                             ttACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAAGCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                                                                                                                                                                                <  1:526587/174‑1 (MQ=255)                                                                                                                  gtacAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                                                                                                                                                                                >  1:842277/4‑169 (MQ=255)                                                                                                                                             ggTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGctacta                                                                                                                                                                  <  1:350690/172‑1 (MQ=255)                                                                                                                                                   gACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                                                                                                                                                                         <  1:795142/95‑3 (MQ=255)                                                                                                                                                   gACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAta                                                                                                                                                                                                                                          >  1:272310/1‑93 (MQ=255)                                                                                                                                                   gACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAAt                                                                                                           <  1:665696/221‑1 (MQ=255)                                                                                                                                                         gagagaTCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                                                                                                                                                                                <  1:449317/130‑1 (MQ=255)                                                                                                                                                         gagagaTCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGtta                                                                        <  1:795950/250‑1 (MQ=255)                                                                                                                                                           gagaTCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAAt                                                                                                                                             <  1:251250/179‑1 (MQ=255)                                                                                                                                                             gaTCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGttactatt                                                                   >  1:44947/1‑251 (MQ=255)                                                                                                                                                                 ctgcaaTGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGGCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAata                                                               <  1:612008/247‑1 (MQ=255)                                                                                                                                                                                       gTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                              >  1:70082/1‑230 (MQ=255)                                                                                                                                                                                           tctcggATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                                                                                                                                                                                >  1:276709/6‑96 (MQ=255)                                                                                                                                                                                             tcggATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATGTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGa                                   <  1:694927/248‑1 (MQ=255)                                                                                                                                                                                                    tACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgcagct                                                                                                                                                                                                       <  1:271890/80‑8 (MQ=255)                                                                                                                                                                                                      cGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTcagt                          <  1:205908/251‑4 (MQ=255)                                                                                                                                                                                                            ataCGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGttactatt                                                                   <  1:662688/204‑1 (MQ=255)                                                                                                                                                                                                              aCGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTcagt                          >  1:113262/1‑240 (MQ=255)                                                                                                                                                                                                                   acataacataAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                              <  1:578923/202‑1 (MQ=255)                                                                                                                                                                                                                   acataacataAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAatat                                                              >  1:254665/1‑202 (MQ=255)                                                                                                                                                                                                                     ataacataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATaag           <  1:527838/251‑2 (MQ=255)                                                                                                                                                                                                                      taacataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAg                                                                           <  1:320163/186‑1 (MQ=255)                                                                                                                                                                                                                        acataaGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAata                                                               >  1:341250/1‑196 (MQ=255)                                                                                                                                                                                                                               tcaGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAATGAAACCTGCTAAATCATTCATGATTTTTTTAATTTTGGATTTTAAAATAGAACCAATTAAAATTCCTGGAATAACAATAGTTACTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAGCTGTCAGTAGTTTTATCAATAAGTGAATAAAg  <  1:432260/248‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                GCGTACCAAGCTTTTCTTCTATAGCTAATAATTGAATTTCTCTAATATCAGTCGATAGTTGAATTAATTGATGATAAATAGAGGAACCAAAATTCAGACTTTGCTGCATTACAGTGCAAAACATCCTGATTTCCGTTGTTGGTAAAGACACAGAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAATGAATCCGGCTAAATCATTCATGATTTTTTTAATTTTAGCTTTCAAAATAGAACCAATTAAAACCCCAGGAATGACAATAGTTATTATTAATATAGTTAGAAAGCCAAGTAACAGAGATGAACTGTCGGTAGTTTCATCAGTAAATGAATAAAG  >  1/853676‑854147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑