Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,809	C→T	intergenic (‑4152/‑68409)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,809	0	C	T	100.0%	137.3 / NA	53	intergenic (‑4152/‑68409)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/32);  total (21/32)

TGTGGAAATACCCTCATTACGCCTGGAGCTAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGATACTCATTTTTGCTGACAGCGTACCAAGCTTTTCTTCTATAGCTAATAATTGAATTTCTCTAATATCAGTCGATAGTTGAATTAATTGATGATAAATAGAGGAACCAAAATTCAGACTTTGCTGCATTACAGTGCAAAACATCCTGATTTCCGTTGTTGGTAAAGACACAGAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAAT  >  1/853582‑853994                                                                                                                                                                                                                                    |                                                                                                                                                                                          tgtgGAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagagag                                                                                                                                                                    <  1:821149/251‑1 (MQ=255)   tgGAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGAt                                                                                                                                                                  <  1:530449/251‑1 (MQ=255)     gAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATc                                                                                                                                                                 >  1:800543/1‑250 (MQ=255)        aTACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTgc                                                                                                                                                             <  1:131174/251‑2 (MQ=255)             cTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGc                                                                                                                                                        <  1:6919/251‑1 (MQ=255)              tCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagag                                                                                                                                                                      <  1:75071/236‑1 (MQ=255)               cATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGc                                                                                                                                                        >  1:601724/1‑249 (MQ=255)               cATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGc                                                                                                                                                        >  1:703287/1‑249 (MQ=255)                    caccTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 <  1:525042/249‑8 (MQ=255)                    caccTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 <  1:504702/249‑8 (MQ=255)                                                  gAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGaataat                                                                                                                    <  1:652373/250‑1 (MQ=255)                                                      cATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGta                                                                                                               >  1:320575/1‑251 (MQ=255)                                                       ataataAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 >  1:503833/1‑209 (MQ=255)                                                        taataaATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGt                                                                                                                >  1:592315/1‑248 (MQ=255)                                                            aaataGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATAccgg                                                                                                                                          >  1:881872/1‑216 (MQ=255)                                                               taGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:372986/251‑1 (MQ=255)                                                               taGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:206450/251‑1 (MQ=255)                                                                  aaTCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 >  1:31248/1‑198 (MQ=255)                                                                   aTCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGt                                                                                                  >  1:371500/1‑251 (MQ=255)                                                                   aTCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGT‑AAGACACAGAGAGATCCTGCAATGCCTGCGACATAc                                                                                                                                             >  1:201559/1‑207 (MQ=255)                                                                     cAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTa                                                                                                                                        >  1:212729/1‑211 (MQ=255)                                                                     cAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGtc                                                                                                 >  1:700864/1‑249 (MQ=255)                                                                            acaCTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGa                                                                                                                         <  1:599371/217‑1 (MQ=255)                                                                                 cATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagagag                                                                                                                                                                    <  1:149011/171‑1 (MQ=255)                                                                                            aCAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      >  1:33746/1‑222 (MQ=255)                                                                                               gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:483186/217‑1 (MQ=255)                                                                                               gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATc                                                                                            <  1:575591/227‑1 (MQ=255)                                                                                               gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                <  1:748897/239‑3 (MQ=255)                                                                                                 gTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGATATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                <  1:344678/239‑3 (MQ=255)                                                                                                 gTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAt                                                                                  >  1:644185/1‑237 (MQ=255)                                                                                                   aCCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAg                                                                         <  1:566096/244‑1 (MQ=255)                                                                                                      aagtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                <  1:717775/230‑3 (MQ=255)                                                                                                        gtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagagag                                                                                                                                                                    <  1:718742/146‑1 (MQ=255)                                                                                                        gtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGtaac                                                                                                             <  1:577912/201‑1 (MQ=255)                                                                                                              cttctATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      >  1:92998/1‑204 (MQ=255)                                                                                                                   ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                  <  1:238065/251‑4 (MQ=255)                                                                                                                   ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                  <  1:723457/251‑4 (MQ=255)                                                                                                                   ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                  <  1:49593/251‑4 (MQ=255)                                                                                                                             atTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:276567/189‑1 (MQ=255)                                                                                                                                      ctctAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAggg                                                                                        <  1:657193/194‑2 (MQ=255)                                                                                                                                                 gTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATc                                                                                            >  1:474858/1‑179 (MQ=255)                                                                                                                                                      tagctgaactagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAgggt                                                                                       <  1:599117/167‑3 (MQ=255)                                                                                                                                                           gaactagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAcccattcccg                              <  1:158094/224‑10 (MQ=255)                                                                                                                                                               tagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAAt      <  1:510932/248‑1 (MQ=255)                                                                                                                                                                   tgatgaTAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAAt  <  1:699177/251‑1 (MQ=255)                                                                                                                                                                     atgatAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGtc                                                                                                 <  1:664522/154‑2 (MQ=255)                                                                                                                                                                        atAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGtc                                                                                                 >  1:176079/1‑150 (MQ=255)                                                                                                                                                                               gagGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                >  1:690597/1‑159 (MQ=255)                                                                                                                                                                                                           ttACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCaa                                                               <  1:597789/150‑1 (MQ=255)                                                                                                                                                                                                           ttACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAAGCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                       <  1:526587/174‑1 (MQ=255)                                                                                                                                                                                                                gtacAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATAccgg                                                                                                                                          >  1:144597/4‑68 (MQ=255)                                                                                                                                                                                                                gtacAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                       >  1:842277/4‑169 (MQ=255)                                                                                                                                                                                                                              gATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAg                                                                                                   >  1:650303/1‑95 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                          TGTGGAAATACCCTCATTACGCCTGGAGCTAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGATACTCATTTTTGCTGACAGCGTACCAAGCTTTTCTTCTATAGCTAATAATTGAATTTCTCTAATATCAGTCGATAGTTGAATTAATTGATGATAAATAGAGGAACCAAAATTCAGACTTTGCTGCATTACAGTGCAAAACATCCTGATTTCCGTTGTTGGTAAAGACACAGAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAAT  >  1/853582‑853994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑