Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,791	G→A	intergenic (‑4134/‑68427)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,791	0	G	A	100.0%	151.2 / NA	57	intergenic (‑4134/‑68427)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/35);  total (22/35)

CACAATAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATACCCTCATTACGCCTGGAGCTAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGATACTCATTTTTGCTGACAGCGTACCAAGCTTTTCTTCTATAGCTAATAATTGAATTTCTCTAATATCAGTCGATAGTTGAATTAATTGATGATAAATAGAGGAACCAAAATTCAGACTTTGCTGCATTACAGTGCAAAACATCCTGATTTCCGTTGTTGGTAAAGACACAGAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAAT  >  1/853548‑853994                                                                                                                                                                                                                                                    |                                                                                                                                                                                                            cataaTAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAAc                                                                                                                                                                                                       <  1:421097/247‑1 (MQ=255)                                 cATGTGGAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACaa                                                                                                                                                                                                          >  1:505469/1‑215 (MQ=255)                                   tgtgGAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagagag                                                                                                                                                                    <  1:821149/251‑1 (MQ=255)                                     tgGAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGAt                                                                                                                                                                  <  1:530449/251‑1 (MQ=255)                                       gAAATACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATc                                                                                                                                                                 >  1:800543/1‑250 (MQ=255)                                          aTACCCTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTgc                                                                                                                                                             <  1:131174/251‑2 (MQ=255)                                               cTCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGc                                                                                                                                                        <  1:6919/251‑1 (MQ=255)                                                tCATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagag                                                                                                                                                                      <  1:75071/236‑1 (MQ=255)                                                 cATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGc                                                                                                                                                        >  1:601724/1‑249 (MQ=255)                                                 cATTACACCTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGc                                                                                                                                                        >  1:703287/1‑249 (MQ=255)                                                      caccTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAAc                                                                                                                                                                                                       >  1:488960/3‑197 (MQ=255)                                                      caccTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 <  1:525042/249‑8 (MQ=255)                                                      caccTGGAGCCAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 <  1:504702/249‑8 (MQ=255)                                                                      tAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAAc                                                                                                                                                                                                       <  1:541638/181‑1 (MQ=255)                                                                                    gAAACATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGaataat                                                                                                                    <  1:652373/250‑1 (MQ=255)                                                                                        cATAATAAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGta                                                                                                               >  1:320575/1‑251 (MQ=255)                                                                                         ataataAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAAc                                                                                                                                                                                                       >  1:398865/1‑162 (MQ=255)                                                                                         ataataAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTctgc                                                                                                                                                                                         <  1:778107/176‑4 (MQ=255)                                                                                         ataataAATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 >  1:503833/1‑209 (MQ=255)                                                                                          taataaATAGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGt                                                                                                                >  1:592315/1‑248 (MQ=255)                                                                                              aaataGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATAccgg                                                                                                                                          >  1:881872/1‑216 (MQ=255)                                                                                                 taGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:372986/251‑1 (MQ=255)                                                                                                 taGAATCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:206450/251‑1 (MQ=255)                                                                                                    aaTCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGcctgcgac                                                                                                                                                 >  1:31248/1‑198 (MQ=255)                                                                                                     aTCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGt                                                                                                  >  1:371500/1‑251 (MQ=255)                                                                                                     aTCAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGT‑AAGACACAGAGAGATCCTGCAATGCCTGCGACATAc                                                                                                                                             >  1:201559/1‑207 (MQ=255)                                                                                                       cAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTa                                                                                                                                        >  1:212729/1‑211 (MQ=255)                                                                                                       cAAAGGGACACTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGtc                                                                                                 >  1:700864/1‑249 (MQ=255)                                                                                                              acaCTCATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGa                                                                                                                         <  1:599371/217‑1 (MQ=255)                                                                                                                   cATTTTTGCCGACAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagagag                                                                                                                                                                    <  1:149011/171‑1 (MQ=255)                                                                                                                              aCAGTGTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      >  1:33746/1‑222 (MQ=255)                                                                                                                                 gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:483186/217‑1 (MQ=255)                                                                                                                                 gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATc                                                                                            <  1:575591/227‑1 (MQ=255)                                                                                                                                 gtgTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                <  1:748897/239‑3 (MQ=255)                                                                                                                                   gTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGATATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                <  1:344678/239‑3 (MQ=255)                                                                                                                                   gTACCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAt                                                                                  >  1:644185/1‑237 (MQ=255)                                                                                                                                     aCCAAGTTTTTCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAg                                                                         <  1:566096/244‑1 (MQ=255)                                                                                                                                        aagtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                <  1:717775/230‑3 (MQ=255)                                                                                                                                          gtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACagagag                                                                                                                                                                    <  1:718742/146‑1 (MQ=255)                                                                                                                                          gtttttCTTCTATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGtaac                                                                                                             <  1:577912/201‑1 (MQ=255)                                                                                                                                                cttctATAGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      >  1:92998/1‑204 (MQ=255)                                                                                                                                                     ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                  <  1:49593/251‑4 (MQ=255)                                                                                                                                                     ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                  <  1:723457/251‑4 (MQ=255)                                                                                                                                                     ataGCCAATAATTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAgcgc                                                  <  1:238065/251‑4 (MQ=255)                                                                                                                                                          caataatTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACAttcggatt                                                                                                                                                                                              >  1:166484/2‑99 (MQ=255)                                                                                                                                                               atTGAATTTCTCTAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTaacataacat                                                                                                      <  1:276567/189‑1 (MQ=255)                                                                                                                                                                        ctctAATATCAGTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAggg                                                                                        <  1:657193/194‑2 (MQ=255)                                                                                                                                                                                   gTCGATAGCTGAACTAGTTGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATc                                                                                            >  1:474858/1‑179 (MQ=255)                                                                                                                                                                                        tagctgaactagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAgggt                                                                                       <  1:599117/167‑3 (MQ=255)                                                                                                                                                                                             gaactagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAcccattcccg                              <  1:158094/224‑10 (MQ=255)                                                                                                                                                                                                 tagttGATGATAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAAt      <  1:510932/248‑1 (MQ=255)                                                                                                                                                                                                     tgatgaTAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATACCCATTCCCGTTTGTAAATTAACAGCTACTAAATCAAt  <  1:699177/251‑1 (MQ=255)                                                                                                                                                                                                       atgatAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGtc                                                                                                 <  1:664522/154‑2 (MQ=255)                                                                                                                                                                                                          atAAATAGAGGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGtc                                                                                                 >  1:176079/1‑150 (MQ=255)                                                                                                                                                                                                                 gagGAACCAAAATTAAGGCTTTGTTGCATTACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAAtat                                                                                >  1:690597/1‑159 (MQ=255)                                                                                                                                                                                                                                             ttACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAATCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCaa                                                               <  1:597789/150‑1 (MQ=255)                                                                                                                                                                                                                                             ttACAGTACAAAACATTCGGATTTCTGCTGTTGGTAAAGACACAGAGAGATCCTGCAATGCCTGCGACATACCGGTAAGCTCGGATTTACGAATAATACGTAACATAACATAAGTCAGATCAGGGTTTAATATTTTAAAGTCAGTTGCAACGTGCTTTAGCGCAGCTTCAATAc                                       <  1:526587/174‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                            CACAATAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATACCCTCATTACGCCTGGAGCTAGAATTAAAATAATAATTGGAAACATAATAAATAGAATCAAAGGGATACTCATTTTTGCTGACAGCGTACCAAGCTTTTCTTCTATAGCTAATAATTGAATTTCTCTAATATCAGTCGATAGTTGAATTAATTGATGATAAATAGAGGAACCAAAATTCAGACTTTGCTGCATTACAGTGCAAAACATCCTGATTTCCGTTGTTGGTAAAGACACAGAGAGATCCTGTAATGCTTGTGACATACCAGTAATTTCAGATTTACGAATAATACGTAACATAACATAAGTTAGATCAGGATTTAATGTTTTAAAGTCAGTTGCAACCTGTTTTAGTGCGGCCTCAATACTTATCCCTGTTTGTACATTAACAGCTACTAAATCAAT  >  1/853548‑853994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑