Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,382	T→C	intergenic (‑3725/‑68836)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,382	0	T	C	100.0%	237.0 / NA	83	intergenic (‑3725/‑68836)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/44);  total (39/44)

GGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCACTGTTTGTTAGCAACGGCTGTAAATAGAGTAGAGATGAATTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGTGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTACTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTGAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCACAATAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATACCCTCATTACGC  >  1/853148‑853603                                                                                                                                                                                                                                           |                                                                                                                                                                                                                              agCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATcttctt                                                                                                                                                                                                               >  1:110660/2‑251 (MQ=255)      aggttacacCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTAttt                                                                                                                                                                                                          >  1:784453/6‑251 (MQ=255)       ggttacacCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTAtttt                                                                                                                                                                                                         >  1:596161/5‑251 (MQ=255)       ggttacacCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTAtttt                                                                                                                                                                                                         >  1:356027/5‑251 (MQ=255)                 cGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCAt                                                                                                                                                                                                >  1:328006/1‑250 (MQ=255)                   aaGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCAc                                                                                                                                                                                             <  1:734340/251‑1 (MQ=255)                     gATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGa                                                                                                                                                                                           >  1:736431/1‑251 (MQ=255)                     gATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGa                                                                                                                                                                                           >  1:130407/1‑251 (MQ=255)                     gATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGa                                                                                                                                                                                           >  1:738663/1‑251 (MQ=255)                     gATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGa                                                                                                                                                                                           >  1:820390/1‑251 (MQ=255)                     gATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGa                                                                                                                                                                                           >  1:805879/1‑251 (MQ=255)                      aTTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAgg                                                                                                                                                                                                                       <  1:620149/222‑1 (MQ=255)                      aTTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGAt                                                                                                                                                                                          >  1:867545/1‑251 (MQ=255)                        tataGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATAc                                                                                                                                                                                        <  1:625282/251‑1 (MQ=255)                             aCTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAg                                                                                                                                                                                    <  1:185423/250‑1 (MQ=255)                             aCTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAg                                                                                                                                                                                    <  1:218674/250‑1 (MQ=255)                              cTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTg                                                                                                                                                                                  <  1:588992/251‑1 (MQ=255)                                tcaccGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCAc                                                                                                                                                                                             >  1:61582/4‑238 (MQ=255)                                 caccGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGAtt                                                                                                                                                                               <  1:829543/249‑1 (MQ=255)                                 caccGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGAtt                                                                                                                                                                               <  1:505240/249‑1 (MQ=255)                                    cGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAAc                                                                                                                                                                            <  1:326379/251‑1 (MQ=255)                                      tttttAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACg                                                                                                                                                                           >  1:84497/1‑250 (MQ=255)                                          taggAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCg                                                                                                                                                                      >  1:568396/3‑251 (MQ=255)                                                aTGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCgttgttg                                                                                                                                                                <  1:575283/251‑1 (MQ=255)                                                aTGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCgttgttg                                                                                                                                                                <  1:220643/251‑1 (MQ=255)                                                aTGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCgttgttg                                                                                                                                                                <  1:535511/251‑1 (MQ=255)                                                  gttgttAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCgttgttgtt                                                                                                                                                              >  1:355572/1‑251 (MQ=255)                                                        aataaGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGtg                                                                                                                                                          >  1:712451/1‑248 (MQ=255)                                                              cTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATa                                                                                                                                                  <  1:345450/251‑1 (MQ=255)                                                                gTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAACTTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATaaa                                                                                                                                                >  1:186373/1‑251 (MQ=255)                                                                        cTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAg                                                                                                                                               <  1:328193/244‑1 (MQ=255)                                                                           ccAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTa                                                                                                                                     >  1:881516/1‑251 (MQ=255)                                                                                 cttcttGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTagcag                                                                                                                                 >  1:287333/1‑249 (MQ=255)                                                                                   tcttGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCgt                                                                                                                                                                     <  1:401167/211‑1 (MQ=255)                                                                                            cgcTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATAc                                                                                                                                                                                        >  1:37904/1‑183 (MQ=255)                                                                                            cgcTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACAt                                                                                                                                                                                      <  1:395833/185‑1 (MQ=255)                                                                                            cgcTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAg                                                                                                                                                                                    >  1:502687/1‑187 (MQ=255)                                                                                            cgcTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCgttgttgtt                                                                                                                                                              <  1:731893/209‑1 (MQ=255)                                                                                            cgcTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAg                                                                                                                     >  1:736743/1‑250 (MQ=255)                                                                                              cttaaGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCAc                                                                                                                  <  1:599169/247‑1 (MQ=255)                                                                                                  aGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATAc                                                                                                                                                                                        <  1:712612/177‑1 (MQ=255)                                                                                                    ttGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAAt                                                                                                             <  1:80548/250‑1 (MQ=255)                                                                                                    ttGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATg                                                                                                            <  1:128139/251‑1 (MQ=255)                                                                                                      gATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGc                                                                                                                                                                          >  1:613271/1‑187 (MQ=255)                                                                                                               ttcgtgcttgTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTAttt                                                                                                 <  1:886743/247‑2 (MQ=255)                                                                                                                     cttgTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTAc                                                                                            <  1:900333/250‑1 (MQ=255)                                                                                                                             attttGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACa                                                                                   >  1:791705/3‑251 (MQ=255)                                                                                                                                     cctttCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTaa                                                                           >  1:648776/3‑251 (MQ=255)                                                                                                                                      ctttCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAAc                                                                          <  1:700887/250‑1 (MQ=255)                                                                                                                                        ttCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAg                                                                        <  1:460884/251‑1 (MQ=255)                                                                                                                                        ttCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAg                                                                        <  1:662157/251‑1 (MQ=255)                                                                                                                                         tCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATATTTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTAcagc                                                                                         >  1:346068/1‑233 (MQ=255)                                                                                                                                         tCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAgg                                                                                                                                                                                                                       <  1:214637/107‑1 (MQ=255)                                                                                                                                         tCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAg                                                                                                                                                            <  1:385799/166‑1 (MQ=255)                                                                                                                                         tCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTAc                                                                                            <  1:267253/230‑1 (MQ=255)                                                                                                                                              tAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGa                                                                  <  1:620848/251‑1 (MQ=255)                                                                                                                                              tAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGa                                                                  <  1:385054/251‑1 (MQ=255)                                                                                                                                                aCTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATc                                                                <  1:887131/251‑1 (MQ=255)                                                                                                                                                   gCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATg                                                             >  1:245515/1‑251 (MQ=255)                                                                                                                                                            ttgttAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAAt                                                    <  1:100346/251‑1 (MQ=255)                                                                                                                                                                        gCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAAt                                                    <  1:701171/239‑1 (MQ=255)                                                                                                                                                                        gCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAAc                                               <  1:112687/244‑1 (MQ=255)                                                                                                                                                                          tGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAag                                      <  1:145183/251‑1 (MQ=255)                                                                                                                                                                            taagtagagtagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAg                                                                                                                                               >  1:235941/5‑144 (MQ=255)                                                                                                                                                                               gtagagtagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGcc                                                           <  1:826923/224‑1 (MQ=255)                                                                                                                                                                                  gagtagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGtgc                                                                                                                                                         >  1:552797/1‑126 (MQ=255)                                                                                                                                                                                  gagtagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTAttt                                                                                                 >  1:727457/1‑183 (MQ=255)                                                                                                                                                                                  gagtagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAAGAGATTTAg                              >  1:26574/1‑251 (MQ=255)                                                                                                                                                                                     tagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAAGAGATTTAGaa                            <  1:779480/250‑1 (MQ=255)                                                                                                                                                                                     tagagATGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGCGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAAGAGATTTAGaa                            <  1:449320/250‑1 (MQ=255)                                                                                                                                                                                         gaTGAACTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAAc                                                                                                                                                                            <  1:392961/102‑1 (MQ=255)                                                                                                                                                                                            gaactACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACAt                                                                                                                                                                                      >  1:614951/5‑89 (MQ=255)                                                                                                                                                                                            gaactACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAg                                                  >  1:505275/5‑221 (MQ=255)                                                                                                                                                                                            gaactACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAAGAGATTTAGaa                            >  1:46744/5‑243 (MQ=255)                                                                                                                                                                                                    gTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTAttt                                                                                                                                                                                                          >  1:364303/1‑61 (MQ=255)                                                                                                                                                                                                     tCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATAccc           <  1:75869/251‑1 (MQ=255)                                                                                                                                                                                                                gTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAg                                                  <  1:771240/201‑1 (MQ=255)                                                                                                                                                                                                                gTAATAAATTTCAGATAATTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAATAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATACCCTCATTAcac  >  1:895961/1‑247 (MQ=255)                                                                                                                                                                                                                                 aTTTATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTAc                                                                                            >  1:358929/1‑142 (MQ=255)                                                                                                                                                                                                                                  tttATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAg                                                                                                                                               <  1:672667/90‑1 (MQ=255)                                                                                                                                                                                                                                  tttATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTAc                                                                                            >  1:132451/1‑141 (MQ=255)                                                                                                                                                                                                                                  tttATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGa                                                                  <  1:212542/167‑1 (MQ=255)                                                                                                                                                                                                                                  tttATAACGCGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTGCTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTTAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCATAAt                                                    >  1:529295/1‑181 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                              GGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCACTGTTTGTTAGCAACGGCTGTAAATAGAGTAGAGATGAATTACTGTCCCCTTTCTTGTAATAAATTTCAGATAATTTATAACGTGTGAGAGGATCTTCTTTATTTTTAAGTCCATCACGATACATAGTGATTAACGCATCGTTGTTGTTAGTACTCTGATAAAGTGCTTCTTTAGCAGCTAATTTATCAGCACTTAATGGTTTTTTATTGAGTACAGCAGAACATGCAGTAACAGATAAGATCATGCCGCACAATAGAACATTTTTAAGAGATTTAGAATACATGTGGAAATACCCTCATTACGC  >  1/853148‑853603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑