Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,124	G→C	intergenic (‑3467/‑69094)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,124	0	G	C	100.0%	161.6 / NA	59	intergenic (‑3467/‑69094)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (37/22);  total (37/22)

ATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCACTGTTTGTTAGCAACGGCTGTAAATAGAGTAGAGATGAATTACTGTCCCCTTTCTTGTAAT  >  1/852878‑853359                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            atcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCtt                                                                                                                                                                                                                                         >  1:590839/1‑251 (MQ=255)          tgtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAt                                                                                                                                                                                                                                <  1:702045/247‑1 (MQ=255)           gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAtt                                                                                                                                                                                                                               >  1:276481/4‑251 (MQ=255)                                                                                                     tGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                                                                                                     <  1:73577/251‑1 (MQ=255)                                                                                                             aactccATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                                             <  1:289089/247‑1 (MQ=255)                                                                                                                    tttAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCtt                                                                                                                      >  1:844866/1‑251 (MQ=255)                                                                                                                       aaaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCttaag                                                                                                                   >  1:829723/1‑248 (MQ=255)                                                                                                                        aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                                                  <  1:802420/251‑1 (MQ=255)                                                                                                                        aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                                                  <  1:266487/251‑1 (MQ=255)                                                                                                                        aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                                                  <  1:572086/251‑1 (MQ=255)                                                                                                                                ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                                                                                                     >  1:57228/4‑224 (MQ=255)                                                                                                                                ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                                                                                                          >  1:27851/4‑251 (MQ=255)                                                                                                                                ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                                                                                                          >  1:589520/4‑251 (MQ=255)                                                                                                                                    ataataATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                                                  >  1:566666/1‑239 (MQ=255)                                                                                                                                        taaTGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                                             >  1:378780/2‑224 (MQ=255)                                                                                                                                             aaaCAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTg                                                                                                                               <  1:784040/217‑1 (MQ=255)                                                                                                                                                  gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat                                                                                        >  1:858571/1‑250 (MQ=255)                                                                                                                                                  gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat                                                                                        >  1:557515/1‑250 (MQ=255)                                                                                                                                                    aTTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaattt                                                                                      <  1:297629/251‑4 (MQ=255)                                                                                                                                                       aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg                                                                                   >  1:151741/1‑251 (MQ=255)                                                                                                                                                       aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg                                                                                   >  1:117556/1‑251 (MQ=255)                                                                                                                                                          aTAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc                                                                                 >  1:620741/1‑250 (MQ=255)                                                                                                                                                                     taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGGAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTaa                                                                      >  1:18251/5‑250 (MQ=255)                                                                                                                                                                     taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc                                                                     <  1:535635/247‑1 (MQ=255)                                                                                                                                                                     taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc                                                                     <  1:704594/247‑1 (MQ=255)                                                                                                                                                                         gATACTTAGCATAGCCATATTATTTACTGCCACTGCATCACTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGAc                                                                                                                                              <  1:365875/174‑1 (MQ=255)                                                                                                                                                                          aTACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGcc                                                                 >  1:826444/1‑250 (MQ=255)                                                                                                                                                                          aTACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGcc                                                                 >  1:625293/1‑250 (MQ=255)                                                                                                                                                                           tACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc                                                                                 >  1:522092/1‑233 (MQ=255)                                                                                                                                                                            aCTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGAc                                                                                                                                              >  1:865588/1‑171 (MQ=255)                                                                                                                                                                               tagcatagccATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGACTTTTGGCCCTTTCACTTAACTGcccg                                                               >  1:157384/1‑246 (MQ=255)                                                                                                                                                                                agcatagccATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                                                  >  1:585531/1‑195 (MQ=255)                                                                                                                                                                                    tagccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCtgtttgtt                                                      >  1:568789/24‑251 (MQ=255)                                                                                                                                                                                     agccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc                                                                     <  1:362741/213‑1 (MQ=255)                                                                                                                                                                                      gccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCtgtttgtt                                                      <  1:691556/228‑1 (MQ=255)                                                                                                                                                                                      gccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAg                                                    >  1:364789/22‑251 (MQ=255)                                                                                                                                                                                      gccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAg                                                    >  1:620879/22‑251 (MQ=255)                                                                                                                                                                                        catattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCa                                                  <  1:23537/232‑1 (MQ=255)                                                                                                                                                                                              atttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTGCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACgg                                              >  1:784591/14‑249 (MQ=255)                                                                                                                                                                                              atttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGc                                                   >  1:352841/14‑244 (MQ=255)                                                                                                                                                                                              atttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACgg                                              >  1:854547/14‑249 (MQ=255)                                                                                                                                                                                                   ctgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTaag                                       <  1:674347/243‑2 (MQ=255)                                                                                                                                                                                                    tgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTaagt                                      <  1:652437/244‑3 (MQ=255)                                                                                                                                                                                                      ccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                                             >  1:718475/6‑162 (MQ=255)                                                                                                                                                                                                         ctgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGtagagt                                 >  1:173912/3‑251 (MQ=255)                                                                                                                                                                                                            catcatTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAagtagagtag                               <  1:671526/250‑1 (MQ=255)                                                                                                                                                                                                              tcatttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga                            <  1:89663/248‑1 (MQ=255)                                                                                                                                                                                                              ccatttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga                            <  1:628643/248‑1 (MQ=255)                                                                                                                                                                                                               catttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAagtagagtag                               >  1:590627/3‑247 (MQ=255)                                                                                                                                                                                                               catttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGAt                           >  1:631311/3‑251 (MQ=255)                                                                                                                                                                                                                   taagaaatattctcgtgCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGaac                       <  1:627529/235‑2 (MQ=255)                                                                                                                                                                                                                           attctcgtgCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga                            <  1:781689/230‑1 (MQ=255)                                                                                                                                                                                                                            ttctcgtgCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTcc                                                                                                                                          >  1:422245/8‑127 (MQ=255)                                                                                                                                                                                                                                        gttaatatCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                                             >  1:74740/5‑128 (MQ=255)                                                                                                                                                                                                                                        gttaatatCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga                            >  1:641194/5‑225 (MQ=255)                                                                                                                                                                                                                                        gttaatatCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga                            >  1:622692/5‑225 (MQ=255)                                                                                                                                                                                                                                        gttaatatCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGATGAACTACTGTCCCCTTTCTTGtaat  >  1:264888/5‑251 (MQ=255)                                                                                                                                                                                                                                                  tCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTaagt                                      <  1:521618/205‑3 (MQ=255)                                                                                                                                                                                                                                                  tCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAagtagagtag                               >  1:363830/1‑212 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            ATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCACTGTTTGTTAGCAACGGCTGTAAATAGAGTAGAGATGAATTACTGTCCCCTTTCTTGTAAT  >  1/852878‑853359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑