Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,091	A→G	intergenic (‑3434/‑69127)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,091	0	A	G	100.0%	164.9 / NA	57	intergenic (‑3434/‑69127)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/23);  total (34/23)

TTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCACTGTTTGTTAGCAACGGCTGTAAATAGAGTAGAGAT  >  1/852853‑853334                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    ttttcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattct                                                                                                                                                                                                                                            <  1:358053/248‑5 (MQ=255)               ttaactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                                                                                                                                                                                                                           <  1:675143/251‑1 (MQ=255)                taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAatat                                                                                                                                                                                                                            >  1:888312/5‑249 (MQ=255)                   ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                                                                                                                                                                                                                           >  1:558830/2‑247 (MQ=255)                        tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGttcg                                                                                                                                                                                                                   >  1:243384/1‑248 (MQ=255)                          atcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCtt                                                                                                                                                                                                                >  1:590839/1‑251 (MQ=255)                                   tgtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAt                                                                                                                                                                                                       <  1:702045/247‑1 (MQ=255)                                    gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattc                                                                                                                                                                                                                                             <  1:33921/209‑4 (MQ=255)                                    gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAtt                                                                                                                                                                                                      >  1:276481/4‑251 (MQ=255)                                            gTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGtt                                                                                                                                                                                                                     <  1:664567/228‑1 (MQ=255)                                                                                                                              tGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                                                                            <  1:73577/251‑1 (MQ=255)                                                                                                                                      aactccATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                    <  1:289089/247‑1 (MQ=255)                                                                                                                                             tttAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCtt                                                                                             >  1:844866/1‑251 (MQ=255)                                                                                                                                                aaaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCttaag                                                                                          >  1:829723/1‑248 (MQ=255)                                                                                                                                                 aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                         <  1:802420/251‑1 (MQ=255)                                                                                                                                                 aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                         <  1:266487/251‑1 (MQ=255)                                                                                                                                                 aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                         <  1:572086/251‑1 (MQ=255)                                                                                                                                                         ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                                                                            >  1:57228/4‑224 (MQ=255)                                                                                                                                                         ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                                                                                 >  1:27851/4‑251 (MQ=255)                                                                                                                                                         ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                                                                                 >  1:589520/4‑251 (MQ=255)                                                                                                                                                             ataataATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                         >  1:566666/1‑239 (MQ=255)                                                                                                                                                                 taaTGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                    >  1:378780/2‑224 (MQ=255)                                                                                                                                                                      aaaCAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTg                                                                                                      <  1:784040/217‑1 (MQ=255)                                                                                                                                                                           gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat                                                               >  1:557515/1‑250 (MQ=255)                                                                                                                                                                           gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat                                                               >  1:858571/1‑250 (MQ=255)                                                                                                                                                                             aTTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaattt                                                             <  1:297629/251‑4 (MQ=255)                                                                                                                                                                                aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg                                                          >  1:151741/1‑251 (MQ=255)                                                                                                                                                                                aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg                                                          >  1:117556/1‑251 (MQ=255)                                                                                                                                                                                   aTAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc                                                        >  1:620741/1‑250 (MQ=255)                                                                                                                                                                                              taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGGAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTaa                                             >  1:18251/5‑250 (MQ=255)                                                                                                                                                                                              taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc                                            <  1:535635/247‑1 (MQ=255)                                                                                                                                                                                              taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc                                            <  1:704594/247‑1 (MQ=255)                                                                                                                                                                                                  gATACTTAGCATAGCCATATTATTTACTGCCACTGCATCACTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGAc                                                                                                                     <  1:365875/174‑1 (MQ=255)                                                                                                                                                                                                   aTACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGcc                                        >  1:625293/1‑250 (MQ=255)                                                                                                                                                                                                   aTACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGcc                                        >  1:826444/1‑250 (MQ=255)                                                                                                                                                                                                    tACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc                                                        >  1:522092/1‑233 (MQ=255)                                                                                                                                                                                                     aCTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGAc                                                                                                                     >  1:865588/1‑171 (MQ=255)                                                                                                                                                                                                        tagcatagccATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGACTTTTGGCCCTTTCACTTAACTGcccg                                      >  1:157384/1‑246 (MQ=255)                                                                                                                                                                                                         agcatagccATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                                                         >  1:585531/1‑195 (MQ=255)                                                                                                                                                                                                             tagccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCtgtttgtt                             >  1:568789/24‑251 (MQ=255)                                                                                                                                                                                                              agccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc                                            <  1:362741/213‑1 (MQ=255)                                                                                                                                                                                                               gccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCtgtttgtt                             <  1:691556/228‑1 (MQ=255)                                                                                                                                                                                                               gccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAg                           >  1:364789/22‑251 (MQ=255)                                                                                                                                                                                                               gccatattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAg                           >  1:620879/22‑251 (MQ=255)                                                                                                                                                                                                                 catattatttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCa                         <  1:23537/232‑1 (MQ=255)                                                                                                                                                                                                                       atttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTGCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACgg                     >  1:784591/14‑249 (MQ=255)                                                                                                                                                                                                                       atttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGc                          >  1:352841/14‑244 (MQ=255)                                                                                                                                                                                                                       atttactgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACgg                     >  1:854547/14‑249 (MQ=255)                                                                                                                                                                                                                            ctgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTaag              <  1:674347/243‑2 (MQ=255)                                                                                                                                                                                                                             tgccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTaagt             <  1:652437/244‑3 (MQ=255)                                                                                                                                                                                                                               ccactgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                                                    >  1:718475/6‑162 (MQ=255)                                                                                                                                                                                                                                  ctgCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGtagagt        >  1:173912/3‑251 (MQ=255)                                                                                                                                                                                                                                     catcatTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAagtagagtag      <  1:671526/250‑1 (MQ=255)                                                                                                                                                                                                                                       tcatttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga   <  1:89663/248‑1 (MQ=255)                                                                                                                                                                                                                                       ccatttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAgaga   <  1:628643/248‑1 (MQ=255)                                                                                                                                                                                                                                        catttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAagtagagtag      >  1:590627/3‑247 (MQ=255)                                                                                                                                                                                                                                        catttAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGCCCGCTGTTTGTTAGCAACGGCTGTAAGTAGAGTAGAGAt  >  1:631311/3‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    TTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCACTGTTTGTTAGCAACGGCTGTAAATAGAGTAGAGAT  >  1/852853‑853334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑