Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,079	A→T	intergenic (‑3422/‑69139)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,079	0	A	T	100.0%	105.1 / NA	40	intergenic (‑3422/‑69139)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/16);  total (24/16)

TTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCA  >  1/852853‑853298                                                                                                                                                                                                                                   |                                                                                                                                                                                                                            ttttcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattct                                                                                                                                                                                                        <  1:358053/248‑5 (MQ=255)               ttaactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                                                                                                                                                                                       <  1:675143/251‑1 (MQ=255)                taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAatat                                                                                                                                                                                        >  1:888312/5‑249 (MQ=255)                 aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCAttt                                                                                                                                                                                                                    <  1:886986/217‑1 (MQ=255)                   ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                                                                                                                                                                                       >  1:558830/2‑247 (MQ=255)                        tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGttcg                                                                                                                                                                               >  1:243384/1‑248 (MQ=255)                          atcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCtt                                                                                                                                                                            >  1:590839/1‑251 (MQ=255)                                   tgtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAt                                                                                                                                                                   <  1:702045/247‑1 (MQ=255)                                    gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattc                                                                                                                                                                                                         <  1:33921/209‑4 (MQ=255)                                    gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAtt                                                                                                                                                                  >  1:276481/4‑251 (MQ=255)                                            gTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGtt                                                                                                                                                                                 <  1:664567/228‑1 (MQ=255)                                                                                                                              tGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                                        <  1:73577/251‑1 (MQ=255)                                                                                                                                      aactccATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                <  1:289089/247‑1 (MQ=255)                                                                                                                                             tttAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCtt                                                         >  1:844866/1‑251 (MQ=255)                                                                                                                                                aaaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCttaag                                                      >  1:829723/1‑248 (MQ=255)                                                                                                                                                 aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                     <  1:572086/251‑1 (MQ=255)                                                                                                                                                 aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                     <  1:802420/251‑1 (MQ=255)                                                                                                                                                 aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                     <  1:266487/251‑1 (MQ=255)                                                                                                                                                         ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                                        >  1:57228/4‑224 (MQ=255)                                                                                                                                                         ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                                             >  1:589520/4‑251 (MQ=255)                                                                                                                                                         ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                                             >  1:27851/4‑251 (MQ=255)                                                                                                                                                             ataataATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                     >  1:566666/1‑239 (MQ=255)                                                                                                                                                                 taaTGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                                                >  1:378780/2‑224 (MQ=255)                                                                                                                                                                      aaaCAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTg                                                                  <  1:784040/217‑1 (MQ=255)                                                                                                                                                                           gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat                           >  1:557515/1‑250 (MQ=255)                                                                                                                                                                           gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat                           >  1:858571/1‑250 (MQ=255)                                                                                                                                                                             aTTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaattt                         <  1:297629/251‑4 (MQ=255)                                                                                                                                                                                aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg                      >  1:117556/1‑251 (MQ=255)                                                                                                                                                                                aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg                      >  1:151741/1‑251 (MQ=255)                                                                                                                                                                                   aTAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc                    >  1:620741/1‑250 (MQ=255)                                                                                                                                                                                              taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGGAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTaa         >  1:18251/5‑250 (MQ=255)                                                                                                                                                                                              taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc        <  1:704594/247‑1 (MQ=255)                                                                                                                                                                                              taatgATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAAc        <  1:535635/247‑1 (MQ=255)                                                                                                                                                                                                  gATACTTAGCATAGCCATATTATTTACTGCCACTGCATCACTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGAc                                                                                 <  1:365875/174‑1 (MQ=255)                                                                                                                                                                                                   aTACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGcc    >  1:826444/1‑250 (MQ=255)                                                                                                                                                                                                   aTACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGCCCTTTCACTTAACTGcc    >  1:625293/1‑250 (MQ=255)                                                                                                                                                                                                    tACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc                    >  1:522092/1‑233 (MQ=255)                                                                                                                                                                                                     aCTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGAc                                                                                 >  1:865588/1‑171 (MQ=255)                                                                                                                                                                                                        tagcatagccATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGACTTTTGGCCCTTTCACTTAACTGcccg  >  1:157384/1‑246 (MQ=255)                                                                                                                                                                                                         agcatagccATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                                     >  1:585531/1‑195 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                            TTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCCTTTTCACTTAACTGCCCA  >  1/852853‑853298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑