Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,045	C→T	intergenic (‑3388/‑69173)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,045	0	C	T	100.0%	118.3 / NA	45	intergenic (‑3388/‑69173)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/23);  total (22/23)

TTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCC  >  1/852816‑853280                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            cctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagc                                                                                                                                                                                                                              <  1:635640/243‑1 (MQ=255)     tttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatattattt                                                                                                                                                                                                                    <  1:506514/251‑11 (MQ=255)       tATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGGCTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTAc                                                                                                                                                                                                                  <  1:635877/251‑1 (MQ=255)           tggtgATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgcc                                                                                                                                                                                                              <  1:616859/247‑5 (MQ=255)                   ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatatta                                                                                                                                                                                                                       <  1:64324/230‑8 (MQ=255)                   ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgcat                                                                                                                                                                                                       >  1:567661/5‑239 (MQ=255)                   ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgcat                                                                                                                                                                                                       >  1:847819/5‑239 (MQ=255)                                     gTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatatta                                                                                                                                                                                                                       >  1:844816/1‑209 (MQ=255)                                     gTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgca                                                                                                                                                                                                        <  1:357793/231‑11 (MQ=255)                                      ttttcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattct                                                                                                                                                                                      <  1:358053/248‑5 (MQ=255)                                                 gCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcat                                                                                                                                                                                                                                 >  1:567019/1‑194 (MQ=255)                                                    ttaactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                                                                                                                                                                     <  1:675143/251‑1 (MQ=255)                                                     taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAatat                                                                                                                                                                      >  1:888312/5‑249 (MQ=255)                                                      aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgca                                                                                                                                                                                                        <  1:67562/211‑11 (MQ=255)                                                      aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCAttt                                                                                                                                                                                                  <  1:886986/217‑1 (MQ=255)                                                        ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagc                                                                                                                                                                                                                                   >  1:540000/2‑185 (MQ=255)                                                        ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                                                                                                                                                                     >  1:558830/2‑247 (MQ=255)                                                             tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGttcg                                                                                                                                                             >  1:243384/1‑248 (MQ=255)                                                               atcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCtt                                                                                                                                                          >  1:590839/1‑251 (MQ=255)                                                                   ggggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatattattt                                                                                                                                                                                                                    <  1:680980/186‑11 (MQ=255)                                                                     ggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagc                                                                                                                                                                                                                                   <  1:415678/171‑1 (MQ=255)                                                                        tgtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAt                                                                                                                                                 <  1:702045/247‑1 (MQ=255)                                                                         gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattc                                                                                                                                                                                       <  1:33921/209‑4 (MQ=255)                                                                         gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAtt                                                                                                                                                >  1:276481/4‑251 (MQ=255)                                                                               aTGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTAc                                                                                                                                                                                                                  <  1:469421/179‑1 (MQ=255)                                                                                 gTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGtt                                                                                                                                                               <  1:664567/228‑1 (MQ=255)                                                                                                                                                                   tGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                      <  1:73577/251‑1 (MQ=255)                                                                                                                                                                           aactccATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                              <  1:289089/247‑1 (MQ=255)                                                                                                                                                                                  tttAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCtt                                       >  1:844866/1‑251 (MQ=255)                                                                                                                                                                                     aaaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCttaag                                    >  1:829723/1‑248 (MQ=255)                                                                                                                                                                                      aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                   <  1:802420/251‑1 (MQ=255)                                                                                                                                                                                      aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                   <  1:266487/251‑1 (MQ=255)                                                                                                                                                                                      aaTACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                   <  1:572086/251‑1 (MQ=255)                                                                                                                                                                                              ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGc                                                      >  1:57228/4‑224 (MQ=255)                                                                                                                                                                                              ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                           >  1:589520/4‑251 (MQ=255)                                                                                                                                                                                              ggcaataataatGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTaa                           >  1:27851/4‑251 (MQ=255)                                                                                                                                                                                                  ataataATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGt                                   >  1:566666/1‑239 (MQ=255)                                                                                                                                                                                                      taaTGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGAt                                              >  1:378780/2‑224 (MQ=255)                                                                                                                                                                                                           aaaCAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTg                                                <  1:784040/217‑1 (MQ=255)                                                                                                                                                                                                                gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat         >  1:557515/1‑250 (MQ=255)                                                                                                                                                                                                                gCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaat         >  1:858571/1‑250 (MQ=255)                                                                                                                                                                                                                  aTTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGaattt       <  1:297629/251‑4 (MQ=255)                                                                                                                                                                                                                     aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg    >  1:117556/1‑251 (MQ=255)                                                                                                                                                                                                                     aCGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTgg    >  1:151741/1‑251 (MQ=255)                                                                                                                                                                                                                        aTAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGATTGCCCATTAAAGCATAGGTTACACCACGAAGATTATAGACTTCACCGTTTTTAGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAACGCTTAAGTTGATTTAAATTTCGTGCTTGTAGAATTTTGGcc  >  1:620741/1‑250 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            TTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATTTCCCATTAAGGCATAAGTCACACCACGAAGATTATAAACTTCCCCGTTTTTGGGAGATGTTGTTAATAAGCTGTTTTCGACTTCCAACGCTTCTTGATAGCGCTTGAGTTGATTTAAATTTCTTGCTTGTAGAACTTTGGCC  >  1/852816‑853280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑