Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,935	A→G	intergenic (‑3278/‑69283)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,935	0	A	G	100.0%	246.5 / NA	85	intergenic (‑3278/‑69283)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/41);  total (44/41)

TGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATT  >  1/852690‑853138                                                                                                                                                                                                                                                       |                                                                                                                                                                                                            ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                                        >  1:59003/2‑250 (MQ=255) ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                                        >  1:209028/2‑250 (MQ=255) ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                                        >  1:87235/2‑250 (MQ=255)  gTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                                        >  1:199123/1‑249 (MQ=255)      tAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGa                                                                                                                                                                                                   >  1:167299/1‑250 (MQ=255)                     aTTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactag                                                                                                                                                                                   >  1:808387/1‑251 (MQ=255)                       tCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                                 <  1:124783/251‑1 (MQ=255)                       tCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                                 <  1:59036/251‑1 (MQ=255)                              ttaagAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTATAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTat                                                                                                                                                                          <  1:785740/247‑2 (MQ=255)                                  gAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAGTATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaa                                                                                                                                                                      >  1:32267/1‑246 (MQ=255)                                   aaTACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                                     <  1:128473/251‑7 (MQ=255)                                              gAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTa                                                                                                                                                          >  1:190547/1‑251 (MQ=255)                                               aaatagaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATtg                                                                                                                                                                               >  1:353629/1‑229 (MQ=255)                                                aatagaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                                        <  1:247510/251‑1 (MQ=255)                                                    gaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCa                                                                                                                                                    >  1:136638/2‑251 (MQ=255)                                                    gaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCa                                                                                                                                                    >  1:523889/2‑251 (MQ=255)                                                    gaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCa                                                                                                                                                    >  1:600419/2‑251 (MQ=255)                                                      ataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagact                                                                                                                                                                                     <  1:872483/216‑3 (MQ=255)                                                      ataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCAtt                                                                                                                                                  >  1:703489/1‑251 (MQ=255)                                                            ccGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATa                                                                                                                                            >  1:886191/1‑251 (MQ=255)                                                                        aCTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                                     >  1:76924/1‑208 (MQ=255)                                                                           cAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataataa                                                                                                                             <  1:203763/251‑4 (MQ=255)                                                                                 ctgacgCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCGATAATAATGAAAc                                                                                                                       <  1:797847/246‑1 (MQ=255)                                                                                 ctgacgCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                                       <  1:355392/246‑1 (MQ=255)                                                                                         cccTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                                 >  1:513584/1‑185 (MQ=255)                                                                                         cccTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAggc                                                                                                                                     >  1:708950/1‑228 (MQ=255)                                                                                           ctttttATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                                        <  1:367785/204‑1 (MQ=255)                                                                                           ctttttATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGa                                                                                                             <  1:710668/247‑1 (MQ=255)                                                                                             ttttATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGTTTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                                       >  1:207168/3‑239 (MQ=255)                                                                                                          gAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaa                                                                                                                                                                      >  1:848082/1‑174 (MQ=255)                                                                                                          gAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                           >  1:204348/1‑206 (MQ=255)                                                                                                                     catttacagaacctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAACCGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATaa                                                                                                          >  1:425489/14‑230 (MQ=255)                                                                                                                        ttacagaacctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                                     <  1:663821/158‑7 (MQ=255)                                                                                                                                cctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagc                                                                                <  1:635640/243‑1 (MQ=255)                                                                                                                                cctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGACATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatg                                                                                              <  1:113517/229‑8 (MQ=255)                                                                                                                                    tttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatattattt                                                                      <  1:506514/251‑11 (MQ=255)                                                                                                                                      tATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGGCTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTAc                                                                    <  1:635877/251‑1 (MQ=255)                                                                                                                                          tggtgATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgcc                                                                <  1:616859/247‑5 (MQ=255)                                                                                                                                               atatTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                                 <  1:731735/133‑1 (MQ=255)                                                                                                                                                tattacTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataat                                                                                                                               <  1:416621/182‑2 (MQ=255)                                                                                                                                                tattacTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                          >  1:132062/1‑208 (MQ=255)                                                                                                                                                tattacTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatg                                                                                              >  1:73800/1‑208 (MQ=255)                                                                                                                                                  ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                                     >  1:765905/5‑136 (MQ=255)                                                                                                                                                  ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                           >  1:547796/5‑168 (MQ=255)                                                                                                                                                  ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatatta                                                                         <  1:64324/230‑8 (MQ=255)                                                                                                                                                  ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgcat                                                         >  1:847819/5‑239 (MQ=255)                                                                                                                                                  ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgcat                                                         >  1:567661/5‑239 (MQ=255)                                                                                                                                                   tactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCAtt                                                                                                                 >  1:287141/4‑193 (MQ=255)                                                                                                                                                   tactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                          >  1:798895/4‑205 (MQ=255)                                                                                                                                                   tactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                          <  1:378192/213‑12 (MQ=255)                                                                                                                                                        gacaCGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                                        >  1:211942/4‑149 (MQ=255)                                                                                                                                                            cgcgTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                           >  1:145916/1‑158 (MQ=255)                                                                                                                                                                    gTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatatta                                                                         >  1:844816/1‑209 (MQ=255)                                                                                                                                                                    gTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgca                                                          <  1:357793/231‑11 (MQ=255)                                                                                                                                                                     ttttcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattct                                        <  1:358053/248‑5 (MQ=255)                                                                                                                                                                        tcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataat                                                                                                                               <  1:399094/156‑2 (MQ=255)                                                                                                                                                                                gCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcat                                                                                   >  1:567019/1‑194 (MQ=255)                                                                                                                                                                                 cATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                           >  1:208026/1‑137 (MQ=255)                                                                                                                                                                                   ttaactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                       <  1:675143/251‑1 (MQ=255)                                                                                                                                                                                    taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                                 <  1:772913/92‑1 (MQ=255)                                                                                                                                                                                    taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAatat                        >  1:888312/5‑249 (MQ=255)                                                                                                                                                                                     aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgca                                                          <  1:67562/211‑11 (MQ=255)                                                                                                                                                                                     aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCAttt                                                    <  1:886986/217‑1 (MQ=255)                                                                                                                                                                                       ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagc                                                                                     >  1:540000/2‑185 (MQ=255)                                                                                                                                                                                       ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc                       >  1:558830/2‑247 (MQ=255)                                                                                                                                                                                            tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAggca                                                                                                                                    <  1:560228/133‑3 (MQ=255)                                                                                                                                                                                            tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                                       >  1:759281/1‑146 (MQ=255)                                                                                                                                                                                            tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGttcg               >  1:243384/1‑248 (MQ=255)                                                                                                                                                                                             catcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                                       >  1:68567/1‑145 (MQ=255)                                                                                                                                                                                              atcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCtt            >  1:590839/1‑251 (MQ=255)                                                                                                                                                                                                  ggggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGa                                                                                                             >  1:56329/4‑150 (MQ=255)                                                                                                                                                                                                  ggggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatattattt                                                                      <  1:680980/186‑11 (MQ=255)                                                                                                                                                                                                    ggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                                        <  1:632153/104‑1 (MQ=255)                                                                                                                                                                                                    ggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagc                                                                                     <  1:415678/171‑1 (MQ=255)                                                                                                                                                                                                       tgtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAt   <  1:702045/247‑1 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagac                                                                                                                                                                                      <  1:837909/68‑2 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTaaa                                                                                                                                              <  1:555913/108‑1 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTaaa                                                                                                                                              <  1:193992/108‑1 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataat                                                                                                                               >  1:350682/4‑125 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTAc                                                                                                               >  1:684838/4‑142 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGa                                                                                                             <  1:859380/141‑1 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                          >  1:740133/4‑152 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                          >  1:690144/4‑152 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTcc                                                                                                     >  1:754052/4‑152 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattc                                         <  1:33921/209‑4 (MQ=255)                                                                                                                                                                                                        gtgttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCTTTATTTGGAtt  >  1:276481/4‑251 (MQ=255)                                                                                                                                                                                                          gttAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCAtt                                                                                                                                                  <  1:739884/104‑1 (MQ=255)                                                                                                                                                                                                              aTGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTAc                                                                    <  1:469421/179‑1 (MQ=255)                                                                                                                                                                                                                gTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGtt                 <  1:664567/228‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                            TGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTTTATTAGGATT  >  1/852690‑853138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑