Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,890	A→G	intergenic (‑3233/‑69328)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,890	0	A	G	100.0%	249.2 / NA	86	intergenic (‑3233/‑69328)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/36);  total (50/36)

TTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTT  >  1/852650‑853128                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGtttt                                                                                                                                                                                                                                      >  1:698914/3‑250 (MQ=255) tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGtttt                                                                                                                                                                                                                                      >  1:586392/3‑250 (MQ=255)   cTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTc                                                                                                                                                                                                                                     >  1:887285/1‑249 (MQ=255)    ttGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCtt                                                                                                                                                                                                                                  <  1:447683/251‑1 (MQ=255)                ttatggctaaatCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATc                                                                                                                                                                                                                      <  1:17258/240‑1 (MQ=255)                             ccaatatgCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCa                                                                                                                                                                                                         >  1:658225/8‑251 (MQ=255)                                  atgCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAAttt                                                                                                                                                                                                     >  1:786540/3‑248 (MQ=255)                                         ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                              >  1:59003/2‑250 (MQ=255)                                         ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                              >  1:87235/2‑250 (MQ=255)                                         ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                              >  1:209028/2‑250 (MQ=255)                                          gTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                                                                                                                                                                                              >  1:199123/1‑249 (MQ=255)                                              tAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGa                                                                                                                                                                                         >  1:167299/1‑250 (MQ=255)                                                           gAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGttt                                                                                                                                                                                                                                       >  1:579971/1‑191 (MQ=255)                                                             aTTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactag                                                                                                                                                                         >  1:808387/1‑251 (MQ=255)                                                               tCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                       <  1:124783/251‑1 (MQ=255)                                                               tCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                       <  1:59036/251‑1 (MQ=255)                                                                    aaTTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATg                                                                                                                                                                                                                                          <  1:386116/179‑1 (MQ=255)                                                                      ttaagAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTATAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTat                                                                                                                                                                <  1:785740/247‑2 (MQ=255)                                                                          gAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAGTATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaa                                                                                                                                                            >  1:32267/1‑246 (MQ=255)                                                                           aaTACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                           <  1:128473/251‑7 (MQ=255)                                                                               cgataaaGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGt                                                                                                                                                                                                                                         >  1:190023/5‑169 (MQ=255)                                                                                      gAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGt                                                                                                                                                                                                                                         <  1:662458/162‑1 (MQ=255)                                                                                      gAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTa                                                                                                                                                >  1:190547/1‑251 (MQ=255)                                                                                       aaatagaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATtg                                                                                                                                                                     >  1:353629/1‑229 (MQ=255)                                                                                        aatagaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                              <  1:247510/251‑1 (MQ=255)                                                                                            gaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCa                                                                                                                                          >  1:600419/2‑251 (MQ=255)                                                                                            gaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCa                                                                                                                                          >  1:136638/2‑251 (MQ=255)                                                                                            gaataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCa                                                                                                                                          >  1:523889/2‑251 (MQ=255)                                                                                              ataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagact                                                                                                                                                                           <  1:872483/216‑3 (MQ=255)                                                                                              ataCAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCAtt                                                                                                                                        >  1:703489/1‑251 (MQ=255)                                                                                                    ccGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATa                                                                                                                                  >  1:886191/1‑251 (MQ=255)                                                                                                                aCTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                           >  1:76924/1‑208 (MQ=255)                                                                                                                 cTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCt                                                                                                                                                                                                                     >  1:445809/1‑155 (MQ=255)                                                                                                                   cAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataataa                                                                                                                   <  1:203763/251‑4 (MQ=255)                                                                                                                         ctgacgCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCGATAATAATGAAAc                                                                                                             <  1:797847/246‑1 (MQ=255)                                                                                                                         ctgacgCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                             <  1:355392/246‑1 (MQ=255)                                                                                                                                 cccTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                       >  1:513584/1‑185 (MQ=255)                                                                                                                                 cccTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAggc                                                                                                                           >  1:708950/1‑228 (MQ=255)                                                                                                                                   ctttttATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                              <  1:367785/204‑1 (MQ=255)                                                                                                                                   ctttttATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGa                                                                                                   <  1:710668/247‑1 (MQ=255)                                                                                                                                     ttttATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGTTTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                             >  1:207168/3‑239 (MQ=255)                                                                                                                                                  gAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaa                                                                                                                                                            >  1:848082/1‑174 (MQ=255)                                                                                                                                                  gAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                 >  1:204348/1‑206 (MQ=255)                                                                                                                                                             catttacagaacctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAACCGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATaa                                                                                                >  1:425489/14‑230 (MQ=255)                                                                                                                                                                ttacagaacctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                           <  1:663821/158‑7 (MQ=255)                                                                                                                                                                        cctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagc                                                                      <  1:635640/243‑1 (MQ=255)                                                                                                                                                                        cctttttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGACATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatg                                                                                    <  1:113517/229‑8 (MQ=255)                                                                                                                                                                            tttATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatattattt                                                            <  1:506514/251‑11 (MQ=255)                                                                                                                                                                              tATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGGCTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTAc                                                          <  1:635877/251‑1 (MQ=255)                                                                                                                                                                                  tggtgATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgcc                                                      <  1:616859/247‑5 (MQ=255)                                                                                                                                                                                       atatTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                       <  1:731735/133‑1 (MQ=255)                                                                                                                                                                                        tattacTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataat                                                                                                                     <  1:416621/182‑2 (MQ=255)                                                                                                                                                                                        tattacTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                >  1:132062/1‑208 (MQ=255)                                                                                                                                                                                        tattacTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatg                                                                                    >  1:73800/1‑208 (MQ=255)                                                                                                                                                                                          ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTatcaaac                                                                                                                                                           >  1:765905/5‑136 (MQ=255)                                                                                                                                                                                          ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                 >  1:547796/5‑168 (MQ=255)                                                                                                                                                                                          ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatatta                                                               <  1:64324/230‑8 (MQ=255)                                                                                                                                                                                          ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgcat                                               >  1:847819/5‑239 (MQ=255)                                                                                                                                                                                          ttactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgcat                                               >  1:567661/5‑239 (MQ=255)                                                                                                                                                                                           tactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCAtt                                                                                                       >  1:287141/4‑193 (MQ=255)                                                                                                                                                                                           tactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                >  1:798895/4‑205 (MQ=255)                                                                                                                                                                                           tactaGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTccattaatgatac                                                                                <  1:378192/213‑12 (MQ=255)                                                                                                                                                                                                gacaCGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                              >  1:211942/4‑149 (MQ=255)                                                                                                                                                                                                    cgcgTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                 >  1:145916/1‑158 (MQ=255)                                                                                                                                                                                                            gTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatatta                                                               >  1:844816/1‑209 (MQ=255)                                                                                                                                                                                                            gTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgca                                                <  1:357793/231‑11 (MQ=255)                                                                                                                                                                                                             ttttcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATattct                              <  1:358053/248‑5 (MQ=255)                                                                                                                                                                                                                tcttGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGcaataat                                                                                                                     <  1:399094/156‑2 (MQ=255)                                                                                                                                                                                                                        gCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcat                                                                         >  1:567019/1‑194 (MQ=255)                                                                                                                                                                                                                         cATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATAc                                                                                                                                 >  1:208026/1‑137 (MQ=255)                                                                                                                                                                                                                           ttaactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc             <  1:675143/251‑1 (MQ=255)                                                                                                                                                                                                                            taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat                                                                                                                                                                       <  1:772913/92‑1 (MQ=255)                                                                                                                                                                                                                            taactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAatat              >  1:888312/5‑249 (MQ=255)                                                                                                                                                                                                                             aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTActgccactgca                                                <  1:67562/211‑11 (MQ=255)                                                                                                                                                                                                                             aactaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCAttt                                          <  1:886986/217‑1 (MQ=255)                                                                                                                                                                                                                               ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagc                                                                           >  1:540000/2‑185 (MQ=255)                                                                                                                                                                                                                               ctaaATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATc             >  1:558830/2‑247 (MQ=255)                                                                                                                                                                                                                                    tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAggca                                                                                                                          <  1:560228/133‑3 (MQ=255)                                                                                                                                                                                                                                    tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                             >  1:759281/1‑146 (MQ=255)                                                                                                                                                                                                                                    tcatcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGttcg     >  1:243384/1‑248 (MQ=255)                                                                                                                                                                                                                                     catcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAAc                                                                                                             >  1:68567/1‑145 (MQ=255)                                                                                                                                                                                                                                      atcaGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTTAGCATAGCCATATTATTTACTGCCACTGCATCATTTAAGAAATATTCTCGTGCTTTGTTAATATCTTCGTTCGCtt  >  1:590839/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGa                                                                                                   >  1:56329/4‑150 (MQ=255)                                                                                                                                                                                                                                          ggggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagcatagccatattattt                                                            <  1:680980/186‑11 (MQ=255)                                                                                                                                                                                                                                            ggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAAc                                                                                                                                              <  1:632153/104‑1 (MQ=255)                                                                                                                                                                                                                                            ggaTGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAAGACTAGATTGTGTATCAAACGCTGTTCCCTAACTCCATTTAAATACTGAGGCAATAATAATGAAACAGCATTACGATAATCTCCATTAATGATACTtagc                                                                           <  1:415678/171‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               TTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGATTGTGTACTAAACGCTGTTCCCTAACCCCATTTAAATACTGAGGTAATAACAATGAAACGGCATTACGATAATCTCCGTTGACGATACTTAGCATAGCAAGATTGTTTACCGCTACAGCATCGTTTAAAAAATACTCGCGGGCTTTATTTATATCTTCGTTGGCTT  >  1/852650‑853128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑