Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,722	G→A	intergenic (‑3065/‑69496)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,722	0	G	A	100.0%	121.7 / NA	47	intergenic (‑3065/‑69496)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/18);  total (29/18)

CAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGAT  >  1/852475‑852963                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   caAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGaa                                                                                                                                                                                                                                                 >  1:620439/1‑251 (MQ=255)         ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                                                                                                                                                                         >  1:726941/1‑248 (MQ=255)         ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                                                                                                                                                                         >  1:690141/1‑248 (MQ=255)               aCCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGat                                                                                                                                                                                                                                           >  1:292311/1‑242 (MQ=255)                                      caatttcatAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCaaa                                                                                                                                                                                                             >  1:274106/9‑250 (MQ=255)                                                                aaaCGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                                                                                                                                                                  <  1:111173/251‑1 (MQ=255)                                                                              gccTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAAttttt                                                                                                                                                                    >  1:335046/2‑251 (MQ=255)                                                                                  ataGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                                                                                                                                                                  >  1:371139/3‑233 (MQ=255)                                                                                                   ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                                                                                               <  1:304702/251‑3 (MQ=255)                                                                                                   ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                                                                                               <  1:138808/251‑3 (MQ=255)                                                                                                    tAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                                                                                              <  1:596994/251‑4 (MQ=255)                                                                                                                tgttTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGAtattac                                                                                                                                <  1:179337/251‑2 (MQ=255)                                                                                                                            ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                                                                                              <  1:570457/224‑4 (MQ=255)                                                                                                                            ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                                                                                               <  1:663824/223‑3 (MQ=255)                                                                                                                            ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGtt                                                                                                                    >  1:423956/4‑251 (MQ=255)                                                                                                                                                atcgttaccttccgccgTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCatta                                                                                                >  1:482743/17‑251 (MQ=255)                                                                                                                                                                 ttCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAgggga                                                                               <  1:35572/251‑4 (MQ=255)                                                                                                                                                                     gtttttAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGtg                                                                           >  1:494047/2‑250 (MQ=255)                                                                                                                                                                                tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCctttt                                                                                                                                                                                      >  1:446240/3‑133 (MQ=255)                                                                                                                                                                                tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGAtattac                                                                                                                                <  1:632849/185‑2 (MQ=255)                                                                                                                                                                                tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAatcatc                                                                                     >  1:462452/3‑230 (MQ=255)                                                                                                                                                                                tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGtttt                                                                 >  1:698914/3‑250 (MQ=255)                                                                                                                                                                                tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGtttt                                                                 >  1:586392/3‑250 (MQ=255)                                                                                                                                                                                  cTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTc                                                                >  1:887285/1‑249 (MQ=255)                                                                                                                                                                                   ttGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCtt                                                             <  1:447683/251‑1 (MQ=255)                                                                                                                                                                                      cACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatct                                                                                                                                             <  1:594033/170‑5 (MQ=255)                                                                                                                                                                                               ttatggctaaatCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATc                                                 <  1:17258/240‑1 (MQ=255)                                                                                                                                                                                                  tggctaaatCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGac                                                                                                                           >  1:857768/9‑173 (MQ=255)                                                                                                                                                                                                            ccaatatgCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCa                                    >  1:658225/8‑251 (MQ=255)                                                                                                                                                                                                              aatatgCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGAtattac                                                                                                                                >  1:520550/6‑156 (MQ=255)                                                                                                                                                                                                                 atgCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAAttt                                >  1:786540/3‑248 (MQ=255)                                                                                                                                                                                                                      gtggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                                                                                              >  1:355721/4‑134 (MQ=255)                                                                                                                                                                                                                      gtggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGac                                                                                                                           >  1:81610/4‑153 (MQ=255)                                                                                                                                                                                                                        ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAATTAAATCATCAgggga                                                                               >  1:14055/2‑193 (MQ=255)                                                                                                                                                                                                                        ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                         >  1:209028/2‑250 (MQ=255)                                                                                                                                                                                                                        ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                         >  1:59003/2‑250 (MQ=255)                                                                                                                                                                                                                        ggTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                         >  1:87235/2‑250 (MQ=255)                                                                                                                                                                                                                         gTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                                                                                              <  1:340139/134‑4 (MQ=255)                                                                                                                                                                                                                         gTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCgttatt                         >  1:199123/1‑249 (MQ=255)                                                                                                                                                                                                                             tAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatctggtga                                                                                                                                        <  1:288878/136‑10 (MQ=255)                                                                                                                                                                                                                             tAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGa                    >  1:167299/1‑250 (MQ=255)                                                                                                                                                                                                                                          gAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGttt                                                                  >  1:579971/1‑191 (MQ=255)                                                                                                                                                                                                                                            aTTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactag    >  1:808387/1‑251 (MQ=255)                                                                                                                                                                                                                                              tCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat  <  1:124783/251‑1 (MQ=255)                                                                                                                                                                                                                                              tCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATTTCGTTATTCTTGACTAAAGCAAagactagat  <  1:59036/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATg                                                                     <  1:386116/179‑1 (MQ=255)                                                                                                                                                                                                                                                    aTTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAggg                                                                                 <  1:343193/166‑2 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATCTTTTGCGTAATCAATGTCATTATTTTTGATTAAAGCAAAGATTAGAT  >  1/852475‑852963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑