Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	852,684	C→T	intergenic (‑3027/‑69534)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	852,684	0	C	T	100.0%	98.1 / NA	37	intergenic (‑3027/‑69534)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/16);  total (21/16)

GGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATC  >  1/852439‑852916                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ggctgccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGt                                                                                                                                                                                                                                      >  1:792738/7‑251 (MQ=255)     gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGtgg                                                                                                                                                                                                                                    <  1:373668/247‑3 (MQ=255)     gccTAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTa                                                                                                                                                                                                                                  >  1:481311/3‑251 (MQ=255)        tAAACTTACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATa                                                                                                                                                                                                                               <  1:731563/251‑1 (MQ=255)           acttACCATCTAATAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGat                                                                                                                                                                                                                            <  1:696224/248‑1 (MQ=255)                      aaTAAAGATTTAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGat                                                                                                                                                                                                                            >  1:768307/1‑240 (MQ=255)                            gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGa                                                                                                                                                                                                                             >  1:140087/4‑233 (MQ=255)                            gatttAATACAAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAg                                                                                                                                                                                                           >  1:360761/4‑251 (MQ=255)                                     caAGAGCTCTCACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGaa                                                                                                                                                                                                  >  1:620439/1‑251 (MQ=255)                                             ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                                                                                                                          >  1:726941/1‑248 (MQ=255)                                             ctcACTACCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGataa                                                                                                                                                                                          >  1:690141/1‑248 (MQ=255)                                                   aCCACAATCAACATATTTAACATCAATTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGat                                                                                                                                                                                            >  1:292311/1‑242 (MQ=255)                                                       caATCAACATATTTAACATCATTTTCATAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAg                                                                                                                                                                                                                              <  1:764995/205‑1 (MQ=255)                                                                          caatttcatAACCGCCATTTTTATCCAAACGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCaaa                                                                                                                                                              >  1:274106/9‑250 (MQ=255)                                                                                                    aaaCGGGCAAGATAGCCTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                                                                                                                   <  1:111173/251‑1 (MQ=255)                                                                                                                  gccTATAGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAAttttt                                                                                                                     >  1:335046/2‑251 (MQ=255)                                                                                                                      ataGTTGAGGTTTCTTGATAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTAtt                                                                                                                                   >  1:371139/3‑233 (MQ=255)                                                                                                                                       ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                                                <  1:304702/251‑3 (MQ=255)                                                                                                                                       ataaataaGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                                                <  1:138808/251‑3 (MQ=255)                                                                                                                                        tAAATAAGTTGTTGTTTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                                               <  1:596994/251‑4 (MQ=255)                                                                                                                                                    tgttTTAGAGCTTTTTCAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGAtattac                                                                                 <  1:179337/251‑2 (MQ=255)                                                                                                                                                                ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatc                                                                                               <  1:570457/224‑4 (MQ=255)                                                                                                                                                                ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttat                                                                                                <  1:663824/223‑3 (MQ=255)                                                                                                                                                                ttttcAAATTCTGCAGCATAATCGTTACCTTCCGCCGTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGtt                                                                     >  1:423956/4‑251 (MQ=255)                                                                                                                                                                                    atcgttaccttccgccgTTCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCatta                                                 >  1:482743/17‑251 (MQ=255)                                                                                                                                                                                                     ttCTGTTTTTAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAgggga                                <  1:35572/251‑4 (MQ=255)                                                                                                                                                                                                         gtttttAGCGATCCTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGtg                            >  1:494047/2‑250 (MQ=255)                                                                                                                                                                                                                    tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCctttt                                                                                                                                       >  1:446240/3‑133 (MQ=255)                                                                                                                                                                                                                    tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGAtattac                                                                                 <  1:632849/185‑2 (MQ=255)                                                                                                                                                                                                                    tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAatcatc                                      >  1:462452/3‑230 (MQ=255)                                                                                                                                                                                                                    tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGtttt                  >  1:586392/3‑250 (MQ=255)                                                                                                                                                                                                                    tccTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGtttt                  >  1:698914/3‑250 (MQ=255)                                                                                                                                                                                                                      cTTGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTc                 >  1:887285/1‑249 (MQ=255)                                                                                                                                                                                                                       ttGCACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCtt              <  1:447683/251‑1 (MQ=255)                                                                                                                                                                                                                          cACTTTCAGTTATGGCTAAATCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACCtttttatct                                                                                              <  1:594033/170‑5 (MQ=255)                                                                                                                                                                                                                                   ttatggctaaatCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGACACGCGTTCAGTTTTCTTGAGGGCATTAACTAAATCATCAGGGGATGTGTTAATGTTTTCCTTCACAATAATATc  <  1:17258/240‑1 (MQ=255)                                                                                                                                                                                                                                      tggctaaatCCCAATATGCAGTGGTAATAGATATACGGCAGAATTCTAGAATTAAGAATACGATAAAGAAATAGAATACAACCGTCAAGGCAAACTCAACGGCTGACGCTCCCTTTTTATTTGATAGGAATTTTTCCATTTACAGAACC‑‑TTTTTATCTGGTGATATTACTAGac                                                                            >  1:857768/9‑173 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          GGCCGTCTAAACCTACCATCCAATAAAGACTTAACACAAGAACTCTCACTACCACAATCAACATATTTAACATCAATCTTGTAACCGCCATTTTTATCCAAACGGGCGAGATAACCTAAAGTTGAAGATTCTTGATAAACAAGCTGTTGTTTTAGAGCTTTCTCAAATTCTACCGCATAATCATTACCCTCTGCAGTTCTATTTTTAGCAATTCTTGCACTTTCAGTGATAGCCAAGTCCCAGTACGCAGTTGTAATAGATATACGACAGAATTCTAGAATTAGGAATACGACAAAGAAATAAAATACAACCGTCAAGGCAAACTCAACGGTTGATGCTCCCTTCTTATTCGATAGGAATTTTTTCA‑TTATAGAACCTTTTTTTATCTGGCGATATTATTAGATACGCGTTCAGTTTTTTTGAGAGCATTAATTAAATCATCAGGAGATGTATTAATGTTTTCCTTCACAATAATATC  >  1/852439‑852916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑